Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting amino acid and nucleic acid from shell, hoofs and horns of animal

A technology of amino acid and carapace, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of waste, environmental pollution, hardness, etc., and achieve the effect of low cost, sufficient sources, simple and feasible process

Inactive Publication Date: 2012-07-11
吴河
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Animal carapaces, hooves, horns, etc. contain high protein and are rich in nutrients, but these untreated keratin proteins have disulfide bonds, which are particularly hard and cannot be used directly. Therefore, as waste, their practical value cannot be reflected. Not only cause waste, but also pollute the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting amino acid and nucleic acid from shell, hoofs and horns of animal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Raw materials should be collected fresh, remove spoilage, debris, sand, metal, etc., soak the raw materials for 1.5 hours before production, wash the sand, debris, dry or dry for later use, after the raw materials are crushed, put them into a porous stainless steel frame, and move them into the pressure Keep the kettle at a pressure of 4Kg and a temperature of 175°C for 1.5 hours; release the hot air in the kettle, and when the pressure gauge is 0, open the lid of the kettle, take out the stainless steel frame, and grind the powder through a 70-mesh sieve after drying.

[0020] According to the weight of the sieved powder, add 1.8 times of water to dilute, then add 2.2 times of sodium xylene sulfonate with a concentration of 30%, adjust the pH to 6.9 with hydrochloric acid, then heat to 100°C for 3 hours; cool and filter , the filtrate is transparent yellow-brown, according to the weight of the filtrate, add 2 times of ethanol with a concentration of 95%, stir for 40 min...

Embodiment 2

[0024] Embodiment two, according to the above-mentioned method, when raw material is ox horn, the purity of gained compound amino acid is 60%.

Embodiment 3

[0025] Embodiment three, according to the above-mentioned method, when raw material is mountain tortoise shell and ox horn by weight ratio 1: 1 composition, the purity of gained compound amino acid is 64%.

[0026] The sediment rich in compound amino acids obtained in Example 1 was submitted for inspection, and the results are as follows: (unit: mg / 100g)

[0027]

[0028] The nucleic acid-rich supernatant obtained in Example 1 was submitted for inspection, and the nucleic acid content was 0.09 mg / mg.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting amino acid and nucleic acid from the shell, the hoofs and the horns of an animal, which comprises the following steps of: crushing and pulverizing raw materials, adding 1.5-2.1 times of water to dilute, adding 2-2.4 times of sodium xylenesulfonate with the concentration of 25-35 percent, adjusting the pH value to be 6.6-7.1 with an acid, heating to90-110 DEG C and maintaining for 50-70 min; then, adding 1.5-2.5 times of ethanol with the concentration of 95 percent, stirring for 20-60 min, settling the solution for 50-70 min, filtering, collecting the filtrate, and concentrating in vacuum; centrifugally precipitating to obtain a precipitate rich in compound amino acid and a supernatant fluid rich in nucleic acid; diluting the precipitate with 1.5-2.5 times of distilled water, and drying to obtain white powered compound amino acid; adding 3-5 times of ethanol with the concentration of 99 percent to the supernatant fluid, settling the solution for 1-3 h, removing the ethanol by using a high speed centrifuge, washing the precipitate with ethanol with the concentration of 70 percent, and drying to obtain nucleic acid. The invention has simple and feasible process and can change wastes into valuables, and moreover, the extraction rate is increased by 30-50 times as comparison with that of methods for using bacillus, yeast and animal's internal organs to extract nucleic acid. The invention has sufficient raw material sources and low cost, and is applicable to large-scale production.

Description

technical field [0001] The present invention relates to a method for extracting active ingredients from animal waste, in particular to a method for extracting amino acid and nucleic acid from animal carapace, hooves and horns. Background technique [0002] Protein plays a very important role in the life activities of cells and organisms. [0003] Amino acid is the most basic substance that constitutes biological proteins and is related to life activities. It is the basic unit of protein molecules in organisms and has a close relationship with biological life activities. [0004] Nucleic acid is an indispensable substance for the human body, and it is also a relatively scarce and expensive substance. More than 5,000 human diseases are caused by nucleic acid deficiency or lack. The human body can fully synthesize itself during the growth and development stage, and then the synthesis gradually decreases. But it can be supplemented by external sources. Therefore, nucleic acid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H21/00C07H1/08A61K31/4174A61K31/401A61K31/198A61P3/02
Inventor 吴河
Owner 吴河