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Method for separating genistein from isoflavone genin mixture

A technology of isoflavone aglycone and genistein, which is applied in the field of separation of genistein and genistein from soybean isoflavone aglycone mixture, and can solve the problem of difficult to have the selective extraction ability of soybean isoflavone aglycon homologues, etc. problems, achieve high selective separation ability, reduce pollution, facilitate recycling and utilization

Active Publication Date: 2010-07-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult for traditional organic solvents to have the ability to selectively extract soybean isoflavone aglycon homologues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The commercially available soybean isoflavone crude product (produced by Heilongjiang Jiusan Oil Co., Ltd.), which contains 40% mass fraction of soybean isoflavone glucoside, was recrystallized 4 times with 75% ethanol aqueous solution, washed with water and suction filtered , to obtain a crude product containing soybean isoflavone glycosides after vacuum drying. The obtained crude product containing soybean isoflavone glycosides is hydrolyzed with 1mol / L sulfuric acid as a catalyst, and 75% ethanol aqueous solution as a solvent, and the hydrolysis reaction product solution is filtered to obtain a solid containing soybean isoflavone aglycones The mixture is washed with water until neutral, recrystallized four times with n-butanol, and vacuum-dried to obtain the raw material soybean isoflavone aglycone mixture.

[0044] The raw material soybean isoflavone aglycon mixture (wherein the total weight percent content of daidzein, genistein and glycitein is 95%) and n-propanol...

Embodiment 2

[0046] The preparation of the raw material soybean isoflavone aglycone mixture is the same as that in Example 1, except that the solvent for the recrystallization of soybean isoflavone aglycone is 75% ethanol aqueous solution.

[0047] The raw material soybean isoflavone aglycone mixture (wherein the total weight percent content of daidzein, genistein and glycitein is 90%) and n-amyl alcohol is formulated into the total amount of daidzein, genistein and glycitein Raw material solution with a concentration of 0.5 g / L. With 1-butyl-3-methylimidazolium chloride ([bmim]Cl)-water mixed solvent as extractant ([bmim]Cl molar fraction 65%), with n-amyl alcohol as detergent, extractant, detergent, The flow ratio of the three raw materials is 4:3:2, fractionation extraction is carried out in a fractionation extraction device at 20° C., the extract is collected, and genistein is obtained after n-pentanol back extraction and vacuum concentration. HPLC analysis showed that the purity of g...

Embodiment 3

[0049] The preparation of the raw material soybean isoflavone aglycone mixture, except that the number of recrystallization of soybean isoflavone glucoside is 3 times, and the recrystallization solvent of soybean isoflavone aglycone is an ethanol aqueous solution with a concentration of 75% by volume, the rest of the operations are carried out in the same way example 1.

[0050] Raw material soybean isoflavone aglycone mixture (wherein the total weight percent content of daidzein, genistein and glycitein is 60%) and water is made into the total concentration of daidzein, genistein and glycitein as 0.6 g / L of raw material solution. With 1-hexyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide salt ([hmim][NTf 2 ]) is the extraction agent, water is the detergent, the flow ratio of the extraction agent, the detergent, and the raw material liquid is 3: 2: 1.5, and fractionation extraction is carried out in a fractionation extraction device at 60° C., the extract is collecte...

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PUM

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Abstract

The invention discloses a method for separating genistein from an isoflavone genin mixture, which is characterized by efficiently separating the genistein from the isoflavone genin mixture containing glycintein, genistein and glycitein by using ionic liquid or a binary mixed solvent containing the ionic liquid and other polar solvents as an extracting agent through a fractional extraction method. The method has the advantages of high separation efficiency, little solvent consumption, low production cost, safety, environmental protection, and the like.

Description

technical field [0001] The invention relates to a method for separating genistein, which belongs to the technical field of chemical engineering, in particular to a method for separating genistein from a mixture of soybean isoflavone aglycones. Background technique [0002] The soybean isoflavone aglycone mixture includes daidzein, genistein and glycitein, and the one with the highest activity function is genistein. The structure of genistein is similar to that of estradiol, and it can bind to estrogen receptors to play the role of estrogen. In addition, it also has the effects of preventing and treating osteoporosis, anti-tumor, lowering blood fat, anti-oxidation, and anti-hepatic fibrosis in vitro. A variety of isoflavone products mainly containing genistein have been approved as food for people at home and abroad. [0003] At present, there are three main methods of obtaining genistein: [0004] The first method is to extract directly from plants containing only genistei...

Claims

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Application Information

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IPC IPC(8): C07D311/36C07D311/40
Inventor 任其龙曹义风邢华斌杨启炜杨亦文苏宝根苏云王雯郭斐张芮菡倪晓蕾
Owner ZHEJIANG UNIV
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