Method for improving rice yield traits by using genetic transformation

A rice production technology, applied in the field of genetic engineering, to achieve the effect of increasing production, low cost and high benefit

Inactive Publication Date: 2010-07-21
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on using this group of lrk genes to increase rice yield or significantly improve yield traits

Method used

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  • Method for improving rice yield traits by using genetic transformation
  • Method for improving rice yield traits by using genetic transformation
  • Method for improving rice yield traits by using genetic transformation

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Experimental program
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Effect test

Embodiment 1

[0031] Functional verification of embodiment 1lrk1 gene

[0032] 1) Construction of lrk1 gene expression vector

[0033] Using pCAMBIA1304 as the expression vector, the Dongxiang wild rice lrk1 gene was inserted into the downstream of the 35S constitutive promoter of the pCAMBIA1304 vector. The structure diagram of the expression vector containing the lrk1 gene is as follows:

[0034] 2) lrk1 gene transformation experiments and results

[0035] 9311, one of the main parents of domestic super hybrid rice, is used as the transgenic recipient. The mature seeds of 9311 were dehulled and sterilized and inoculated on dedifferentiated plant tissue culture medium to induce callus. After 2-3 weeks of callus induction, the purified pCAMBIA1304 plasmid DNA containing lrk1 gene was introduced into 9311 callus cells by particle gun bombardment. Resistant cell lines were selected by continuous culture for 3-4 weeks on MS medium supplemented with 30 ppm hygromycin, and then the selected ...

Embodiment 2

[0045] Example 2 Method for transforming rice lrk1 gene into indica rice 9311

[0046] 1) Select the full-length ORF segment of lrk1 (see the sequence list), amplify it from Dongxiang wild rice by PCR method, synthesize an insert linker and clone it into the plant expression vector pCAMBIA1304, and obtain the lrk1 expression vector pCAMBIA1304 / lrk1.

[0047] 2) Culture medium for inducing rice callus

[0048] 1. Induction and subculture medium: MS+2mg / L 2,4-D.

[0049] 2. Hyperosmotic medium: MS+2mg / L 2,4-D+46.67g / L sorbitol+46.67g / L mannitol.

[0050] 3. The first round of screening medium: MS+2mg / L 2,4-D+30mg / L hygromycin.

[0051] 4. The second round of screening medium: MS+2mg / L 2,4-D+50mg / L hygromycin.

[0052] 5. Differentiation medium: MS+3mg / L 6-BA+0.5mg / L NAA+50mg / L hygromycin.

[0053] 6. Rooting and strong seedling medium: 1 / 2MS+0.1mg / L NAA

[0054] Note: 1. The above media all contain 30g / L sucrose + 2.5g / L agar, pH 5.8

[0055] 2. The culture conditions for ...

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Abstract

The invention belongs to the field of genetic engineering, relating to a method for increasing rice yield and yield traits by using lrk1 gene transformation. By using a gene gun microprojectile bombardment method, after recovery cultivation, a rice plant expressing the lrk1 gene is obtained from rice callus genetically transformed by the lrk1 gene. Results show that, compared with a control sample, the successfully transformed rice plant has faster seed germination potential, better seedling stage tillering potential, as well as generally superior seedling stage growth potential and plant ripeness spike index. The invention provides the new method for increasing the rice yield with low cost and high benefit.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a method for improving rice yield and yield traits by using lrk1 gene transformation. Background technique [0002] At present, the techniques and methods of using transgenes to improve crop economic traits at home and abroad are mainly based on the known relationship between functional genes and phenotypic traits, using constitutive expression vectors or tissue-specific expression vectors to introduce target genes into target plants, in order to obtain crops. Improvement of specific economic traits or stress resistance traits. [0003] In crops, especially cereal crops, yield traits are usually controlled by polygenes or quantitative genes. The method of isolating and cloning such yield trait genes generally adopts positional cloning technology, and then verifies its function through transgenic. Using the gene lines constructed by hybridization and backcrossing o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 杨金水何光明罗小金查笑君孙传清朱作峰
Owner FUDAN UNIV
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