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Method for extracting oligo proanthocyanidin from grape seeds

A technology of proanthocyanidins and grape seeds, applied in the direction of organic chemistry, which can solve the problems of high equipment investment cost, complex equipment pressure, high risk, etc., and achieve the effects of saving extraction time, reducing energy consumption, and high extraction rate

Active Publication Date: 2010-07-28
SUZHOU ZEDAXINGBANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the method equipment is more complicated and requires a certain pressure (27±2MPa), and the equipment investment cost is high
Microwave-assisted extraction method (such as CN1102589C) has the characteristics of short extraction time and high thermal efficiency, but it has high risk and poor safety when microwave leaks

Method used

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  • Method for extracting oligo proanthocyanidin from grape seeds
  • Method for extracting oligo proanthocyanidin from grape seeds
  • Method for extracting oligo proanthocyanidin from grape seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Ethanol extraction weigh a certain amount of grape seeds crushed to 10-40 mesh, put them into tank A and add 6-10 times the amount of 35-75% ethanol, the extraction temperature is 45-75°C, and the extraction time is 30-60min. Follow the steps below (see figure 1 ), (the left 1-5 in the figure corresponds to the following steps 1-5), the same amount of materials put in each step, the same concentration of ethanol and the same volume, the extraction temperature and time are also the same, measured by the sulfuric acid-vanillin method The proanthocyanidin content and total phenol content measured by Folin-Shuca method (the same below), the extraction transfer rate is 60-95%. ( figure 1 1-5 on the left side correspond to the following steps 1-5 respectively)

[0017] Step 1. Put a certain amount of grape seeds into tanks A, B, and C; add new solvent to tank A and extract at a set temperature. After the extraction is complete, transfer the extract to tank B;

[0018] Step 2. Add...

Embodiment 2

[0024] Water extraction Weigh a certain amount of whole grape seeds into tank A and add 3-6 times the amount of water. The extraction temperature is 70-100°C and the extraction time is 30-60min. Follow the steps below (see figure 2 ), the same amount of materials and the same volume of water are put in each step, the extraction temperature and time are the same, and the extraction transfer rate is 60-85%. ( figure 2 1-7 on the left side correspond to the following steps 1-7 respectively)

[0025] Step 1. Put a certain amount of grape seeds into tanks A, B, C, and D; add new solvent to tank A and extract at a set temperature. After the extraction is complete, transfer the extract to tank B;

[0026] Step 2. Add new solvent to tank A and extract at the set temperature at the same time as tank B; after extraction is completed, the extract from tank B is delivered to tank C, and the extract from tank A is delivered to tank B;

[0027] Step 3. Add new solvent to tank A, and extract A...

Embodiment 3

[0034] Accurately weigh several portions of 20 mesh grape seeds, each portion is 20 g, the material-liquid ratio is 1:3 to 1:10 (g:ml), the extraction solvent is 35-75% ethanol, and each extraction time is 30 minutes. Design an orthogonal experiment, see Table 1 for factor levels, and extract from 1-5 according to the operation steps of Example 1. After the extraction, the extracts of steps 3, 4, and 5 are combined, the volume is measured, and the content of proanthocyanidins is sampled and analyzed. The results are shown in Table 2. The preferred specific embodiments should be understood to be illustrative only and not to limit the scope of the present invention in any way.

[0035] Table 1 Factor level table

[0036]

[0037] Table 2 Orthogonal test results

[0038]

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Abstract

The invention provides a method for extracting oligo proanthocyanidin from grape seeds, which comprises the following steps: placing materials in extraction tanks, wherein the extraction solvent is water or water-containing ethanol, the using amount is 3-10 times of the volume of material mass, the temperature is 45-100 DEG C, and the time is 20-60 min; leading the content of active ingredients in extraction liquid in all the tanks to be gradually reduced by sequentially extracting the tanks, carrying out liquid discharge on the extraction liquid with the highest content of the active ingredients in all the tanks, entering into the circulating step, then carrying out residue discharge and material addition on the extraction tank which completes a plurality of times of solvent extraction of materials, adding a new solvent during the last extraction of the materials, then transferring by spacing one unit, carrying out a plurality of times of extraction and discharging liquid. The method can ensure that the materials and the solvents of all the extraction units can always keep the greater concentration difference of the active ingredients, accelerate the extraction rate, improve the concentration of the active ingredients of the final solvent and be characterized by saving the solvents, saving energy and the like under the premise of ensuring the higher extraction rate.

Description

Technical field [0001] The invention belongs to the field of plant effective ingredient extraction, and relates to a method for extracting active ingredient proanthocyanidins from grape seeds, in particular to a method for improving the traditional single-tank extraction into a tank-group countercurrent extraction to extract proanthocyanidins from grape seeds. Background technique [0002] Procyanidins are composed of (+)-catechin, (-)-epicatechin and (-)-epicatechin gallate through C 4 -C 8 Key or C 4 -C 6 Polyphenols with different polymerization degrees formed by bonding are widely present in plants such as grapes, blueberries, cherries, plums and pine bark, among which grape seeds are an important source of proanthocyanidins. Proanthocyanidin (GPC) has a strong antioxidant effect and is highly absorbed by the human body. It can remove free radicals and active oxygen in the body and prevent arteriosclerosis caused by the oxidation of low-density lipoprotein (LDL) in human blood...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
Inventor 栾连军陈娜刘雪松吴永江陈勇王龙虎
Owner SUZHOU ZEDAXINGBANG MEDICAL TECH
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