Use of cis-butenedioic anhydride compounds in preparation of Sclerotinia sclerotiorum bacteriostat
A technology of maleic anhydride and Sclerotinia sclerotiorum, applied in the direction of application, bactericide, biocide, etc., can solve problems such as few reports, and achieve the effect of solving the problem of resistance
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Embodiment 1
[0020] Example 1: Screening maleic anhydride compounds with higher antibacterial activity against Sclerotinia sclerotiorum by using mycelium growth rate method
[0021] Using the mycelia growth rate method, the maleic anhydride compounds were formulated into test liquids with different concentration gradients, and 5 mL of the liquid was added to 45 mL of melted PDA medium, mixed evenly, and then poured into a sterilized petri dish. medium (diameter 9cm), pour each bottle evenly into 3 petri dishes, and prepare the poisonous culture medium with the required concentration. As a control, mix the same volume of sterile water or acetone solvent. Use a hole puncher with an inner diameter of 0.6 cm to punch a hole at the edge of the sclerotinia colony that has been cultivated for 3 days, remove the 0.6 cm-diameter cake, and inoculate it in the center of each petri dish, on the side with mycelium face down. Each petri dish was inoculated with a piece of bacteria cake, and each treat...
Embodiment 2
[0027] Embodiment 2: The influence of pH on the activity of maleic anhydride compounds inhibiting Sclerotinia sclerotiorum
[0028] The effect of pH on their antibacterial activity was investigated at half inhibitory concentrations of dimethyl maleic anhydride and biphenyl maleic anhydride (38.9 μg / mL, 37.65 μg / mL). Taking the medium without adding drugs as the blank control, the pH range of the medium is 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0. Each treatment was repeated three times, and cultured in a 25°C incubator for 2 days after inoculation. The diameter of the colony was measured by the cross method, and the inhibition rate was calculated. Experiments were repeated three times. Experimental results such as figure 1 shown.
[0029] The experimental results show that the antibacterial effect of variable dimethyl maleic anhydride is better under acidic conditions, and the optimum pH is 5.0. When the pH of biphenyl maleic anhydride is 5.0 under acidic condition...
Embodiment 3
[0030] Embodiment 3: the impact of maleic anhydride compounds on the formation of sclerotia
[0031] Prepare a series of media containing different concentrations of maleic anhydride compound: the final concentration of 2,3-dimethylmaleic anhydride is 10 μg / mL, 30 μg / mL, 60 μg / mL, 90 μg / mL, 120 μg / mL. The final concentration of biphenyl maleic anhydride was 20 μg / mL, 40 μg / mL, 60 μg / mL, 80 μg / mL, 100 μg / mL, 120 μg / mL, and the medium without drug was used as the control. After inoculation, culture in a 25°C incubator for 10 days, and count the number of sclerotia on each plate. Nine plates were treated for each treatment, and the experiment was repeated 3 times. The results are shown in Table 2.
[0032] Table 2: Effect of maleic anhydride-containing compounds on sclerotia formation
[0033]
[0034]
[0035] *The number of sclerotias is the average number of sclerotias formed in nine plates
[0036] Experimental results show that maleic anhydride compounds can signifi...
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