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Method for improving germination of stevia rebaudiana seeds

A stevia and seed technology, applied in the agricultural field, can solve the problems of affecting stevia field production, loss of germination power, long germination time, etc., to facilitate nutrient absorption, induce and stimulate cell activity, improve the ability to resist adversity, and achieve high germination. The effect of rate and germination vigour

Inactive Publication Date: 2011-08-10
QINGDAO AGRI UNIV
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  • Description
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Problems solved by technology

[0003] Due to the small size of Stevia rebaudiana seeds, the dry grain weight is only 0.25-0.4 grams, and there are few stored nutrients. In the process of Stevia rebaudiana seedling cultivation in early spring, due to the large temperature changes, Stevia rebaudiana seeds are extremely vulnerable to low temperature, chilling damage, high temperature, etc. during the germination process. Under the influence of adversity, it is easy to lose the germination force during the germination process, the germination time is long, the germination rate and germination potential are reduced, the seedlings are not uniform, and the seedlings are poor, which affects the field production of stevia

Method used

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  • Method for improving germination of stevia rebaudiana seeds
  • Method for improving germination of stevia rebaudiana seeds

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Experimental program
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Effect test

Embodiment approach 1

[0012] Embodiment 1: 2,000 grains of hybrid Qingtian No. 4 (selected by Qingdao Agricultural University) were selected, and 1,000 grains were randomly divided into equal parts. Among them, 1000 dry seeds of Qingtian No. 4 and 6.5% polyethylene glycol 4000 aqueous solution were soaked and treated for 30 hours at a temperature of 25°C according to the ratio of 1:9, and then washed with water and air-dried the seeds (dried in the shade); another 1000 The grains were used as the control, and the seeds were not soaked in 6.5% polyethylene glycol 4000 aqueous solution, but were only washed with water and air-dried (dried in the shade). Afterwards, the seeds were transferred to the culture dishes of the paper germ bed, 100 grains per dish, the soaking treatment and the control were repeated 8 times, and then they were placed in a culture room with a constant temperature of 25°C and 12 hours of light, and the germination rate of each treatment was recorded every day. and germination p...

Embodiment approach 2

[0024] Embodiment 2, the selected species is hybrid Qingtian No. 4 (selected by Qingdao Agricultural University) and commercially available stevia seeds. After fully selecting, Qingtian No. 4 and commercially available stevia dry seeds are mixed with 7% polyethylene Alcohol 4000 aqueous solution according to the ratio of 1:7.5, soaked at 26°C for 28 hours, then washed with water and air-dried the seeds (dried in the shade); the control was Qingtian No. 4 and the dry seeds of Stevia rebaudiana on the market did not use 7% Seeds were soaked in polyethylene glycol 4000 aqueous solution, only cleaned with water and air-dried seeds (dry in the shade), and then sowed in the seedbed, after sowing, began to record the emergence rate and emergence potential of each treatment of Qingtian 4 and commercially available dry seeds of Stevia rebaudiana,

[0025] The calculation formula is:

[0026] Emergence rate (%) (seedbed test) = total number of seedlings * 100% / number of seeds for testin...

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Abstract

The invention discloses a method for improving germination of stevia rebaudiana seeds, which relates to a crop seed treatment technique in the technical field of agriculture. The method comprises the following steps of: selecting and sterilizing seeds, preparing 6 to 8 percent aqueous solution of polyethylene glycol 4000, soaking the seeds into the prepared solution at the temperature of between 20 and 30 DEG C for 24 to 48 hours, then filtering the seeds, cleaning the seeds by using clear water, and airing the seeds for later sowing. In the method, the used polyethylene glycol is generally recognized that molecules of the polyethylene glycol can change biological membrane structures of various cells in cell engineering so that lipid molecules on a plasma membrane at a contact point of two cells are dispersed and recombined, can change the osmotic regulation capability of plants on plant physiology and are favorable for absorbing nutrition and inducing the activity of stimulation cells. The method can remarkably improve the capability of resisting adverse situations when the stevia rebaudiana seeds are germinated so that the stevia rebaudiana seeds still maintain higher germination rate and germination energy in adverse situation harm and the seedlings have good quality.

Description

Technical field: [0001] The invention relates to the crop seed treatment technology in the field of agricultural technology. Background technique: [0002] Stevia is a new sugar crop. The sweetness of glycosides contained in the leaves is about 300 times that of sucrose. It is a kind of high sweetness, low calorie and good taste. It can replace part of saccharin and sucrose. It is used in food and beverage middle. Stevia rebaudioside is basically excreted in the original body in the human body, and will not decompose to produce glucose. It is a safe, non-toxic, natural plant-free energy-free sweetener, which can effectively prevent diabetes, obesity, and dental caries in children. The third natural sugar source after sucrose and beet sugar. [0003] Due to the small size of Stevia rebaudiana seeds, the dry grain weight is only 0.25-0.4 grams, and there are few stored nutrients. During the stevia seedling cultivation process in early spring, due to the large temperature cha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
Inventor 石岩马洪义任广喜刘相阳
Owner QINGDAO AGRI UNIV
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