Preparation method of single-green up-conversion fluorescent marking material

A fluorescent labeling and green technology, applied in the field of preparation of fluorescent labeling materials, can solve the problems of low detection sensitivity, complicated preparation process, poor chemical stability, etc., and achieve the effects of low reaction temperature, good monochromaticity, and short reaction time.

Inactive Publication Date: 2010-09-22
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention solves the problems of complicated preparation process, poor chemical stability, easy photodamage, toxicity, low detection sensitivity and poor monochromaticity of the existing fluorescent labeling materials, and provides a preparation method of single green up-conversion fluorescent labeling materials

Method used

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  • Preparation method of single-green up-conversion fluorescent marking material
  • Preparation method of single-green up-conversion fluorescent marking material
  • Preparation method of single-green up-conversion fluorescent marking material

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specific Embodiment approach 1

[0008] Specific embodiment one: the preparation method of the single green up-conversion fluorescent labeling material in the present embodiment is as follows: one, the Y 2 o 3 , Yb 2 o 3 、Ho 2 o 3 and Eu 2 o 3 Dissolve in concentrated nitric acid, add deionized water, then add LiNO 3 solution, stirred evenly at room temperature to obtain a transparent solution, Y in the transparent solution 3+ Ions and Yb 3+ The molar ratio of ions is 1:0~10, Y 3+ Ions and Ho 3+ The molar ratio of ions is 1:0.1~0.5, Y 3+ Ions and Eu 3+ The molar ratio of ions is 1:0~2, Y 3+ Ions and Li + The molar ratio of ions is 1:0~15; 2. According to the molar ratio of cations and citric acid in the transparent solution is 1:3~5, add citric acid to the transparent solution and stir until it is completely dissolved, then add ammonia water to adjust The pH value is 5~7; 3. Dry the solution obtained in step 3 at 100~150°C for 12~20 hours to obtain xerogel; 4. Calcinate the xerogel at 600~1000°C...

specific Embodiment approach 2

[0011] Specific embodiment two: the difference between this embodiment and specific embodiment one is that Y in step one transparent solution 3+ Ions and Yb 3+ The molar ratio of ions is 1:1~9, Y 3+ Ions and Ho 3+ The molar ratio of ions is 1:0.2~0.4, Y 3+ Ions and Eu 3+ The molar ratio of ions is 1:1~1.5, Y 3+ Ions and Li + The molar ratio of ions is 1:1~14. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0012] Specific embodiment three: the difference between this embodiment and specific embodiment one is that Y in step one transparent solution 3+ Ions and Yb 3+ The molar ratio of ions is 1:8, Y 3+ Ions and Ho 3+ The molar ratio of ions is 1:0.3, Y 3+ Ions and Eu 3+ The molar ratio of ions is 1:1.5, Y 3+ Ions and Li + The molar ratio of ions is 1:10. Others are the same as in the first embodiment.

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Abstract

The invention relates to a preparation method of a single-green up-conversion fluorescent marking material, in particular to a preparation method of a fluorescent marking material, solving the problems of complicated preparation process, poor chemical stability, easy light injury, toxicity, low detection sensitivity and poor monochromaticity of a traditional preparation method of the fluorescent marking material. The method comprises the following steps of: dissolving Y2O3, Yb2O3, Ho2O3 and Eu2O3 to concentrated sulfuric acid; adding deionized water; adding an LiNO3 solution; adding a complexing agent citric acid; then, adding ammonia to adjust a pH value; drying to obtain dried gel; calcining the dried gel; and naturally cooling to a room temperature to obtain a single-green up-conversion fluorescent marking material. The method has simple preparation process, low reaction temperature and hardly damaged crystal lattice, the single-green up-conversion fluorescent marking material prepared by using the method has high purity, good evenness of particles in 20-50 nm, strong luminescence and favorable monochromaticity.

Description

technical field [0001] The invention relates to a preparation method of a fluorescent marking material. Background technique [0002] Fluorescent labeling materials can be widely used in high-tech fields such as cell tracking, ion tunneling detection, and DNA detection. The existing fluorescent labeling materials are mainly organic dyes and quantum dots. Organic dyes are toxic, have poor chemical stability, short fluorescence duration, and are not suitable for multi-color observation, etc., while quantum dots will produce strong autofluorescence and affect detection sensitivity. Therefore, they all have their own inherent defects which limit their further wide application. Rare-earth up-conversion fluorescent labeling materials are emitted by converting the excited infrared light into visible light, so high tissue transmittance can be obtained, zero background detection can be achieved, and the detection sensitivity is very high; and the matrix of this material is an inorga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/78
Inventor 刘娟盛艳秋张治国曹文武
Owner HARBIN INST OF TECH
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