Zymocyte mixture containing lucid ganoderma, preparation method and application thereof
A technology for fermenting bacteria and mixtures, applied in the field of bacterial mixtures, can solve the problems of difficult operation, easy pollution and high cost
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[0031] (2) Preparation of the test solution: ①Extract the supernatant: accurately weigh 5g of the fine powder of the sample (approximately equivalent to 0.22g of polysaccharide), put it in a flask, add 100ml of water, put it on an electric stove, heat and reflux for extraction for 1.5h, and let it cool until Centrifuge at room temperature at 3000-4000rpm for 10 minutes to collect the supernatant, wash the flask and centrifuge tube with a small amount of water, and collect the supernatant by centrifugation in the same way. ②Extraction of polysaccharide precipitation: Combine all supernatants in an evaporating dish, heat carefully on an electric stove (to avoid coking), concentrate to about 15ml, let cool to room temperature, add 100ml of ethanol, stir well, and let it settle for 15 minutes. Transfer this solution together with the precipitate into a centrifuge tube, continue to use a small amount of 80% ethanol to fully scrape the evaporating dish with a glass rod, transfer the ...
Embodiment 1
[0115] Solid fermentation medium and culture conditions of Ganoderma lucidum / Salvia miltiorrhiza mixture:
[0116] Medium composition: corn cob 5%, wheat bran 20%, aspartate 5%, salvia 70%.
[0117] Solid fermentation conditions: normal temperature (15-25°C) fermentation for 30 days.
Embodiment 2
[0119] Solid fermentation medium and culture conditions of Ganoderma lucidum / Salvia miltiorrhiza mixture:
[0120] Medium composition: corncob 25%, wheat bran 25%, salvia miltiorrhiza 50%.
[0121] The solid fermentation condition is the same as the above example.
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