Zymocyte mixture containing lucid ganoderma, preparation method and application thereof

A technology for fermenting bacteria and mixtures, applied in the field of bacterial mixtures, can solve the problems of difficult operation, easy pollution and high cost

Active Publication Date: 2010-10-13
成都宇泽生物基因化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, its production research has mainly focused on liquid submerged fermentation, mainly referring to the production process of antibiotics; its production efficiency is high, and the product quality is relatively stable, but there are problems such as high cost, large energy consumption, easy to be polluted, and difficult to operate.

Method used

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  • Zymocyte mixture containing lucid ganoderma, preparation method and application thereof
  • Zymocyte mixture containing lucid ganoderma, preparation method and application thereof
  • Zymocyte mixture containing lucid ganoderma, preparation method and application thereof

Examples

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Effect test

preparation example Construction

[0031] (2) Preparation of the test solution: ①Extract the supernatant: accurately weigh 5g of the fine powder of the sample (approximately equivalent to 0.22g of polysaccharide), put it in a flask, add 100ml of water, put it on an electric stove, heat and reflux for extraction for 1.5h, and let it cool until Centrifuge at room temperature at 3000-4000rpm for 10 minutes to collect the supernatant, wash the flask and centrifuge tube with a small amount of water, and collect the supernatant by centrifugation in the same way. ②Extraction of polysaccharide precipitation: Combine all supernatants in an evaporating dish, heat carefully on an electric stove (to avoid coking), concentrate to about 15ml, let cool to room temperature, add 100ml of ethanol, stir well, and let it settle for 15 minutes. Transfer this solution together with the precipitate into a centrifuge tube, continue to use a small amount of 80% ethanol to fully scrape the evaporating dish with a glass rod, transfer the ...

Embodiment 1

[0115] Solid fermentation medium and culture conditions of Ganoderma lucidum / Salvia miltiorrhiza mixture:

[0116] Medium composition: corn cob 5%, wheat bran 20%, aspartate 5%, salvia 70%.

[0117] Solid fermentation conditions: normal temperature (15-25°C) fermentation for 30 days.

Embodiment 2

[0119] Solid fermentation medium and culture conditions of Ganoderma lucidum / Salvia miltiorrhiza mixture:

[0120] Medium composition: corncob 25%, wheat bran 25%, salvia miltiorrhiza 50%.

[0121] The solid fermentation condition is the same as the above example.

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Abstract

The invention relates to a zymocyte mixture containing lucid ganoderma. A mycoplasm mixture containing hypha and culture matrix components is obtained by fermenting the lucid ganoderma in a solid fermentation mode in a nutritional culture matrix which at least comprises a carbon source and a nitrogen source needed by the growth of fungi, wherein medical raw materials salvia miltiorrhiza are mixed in the culture matrix. A mycoplasm fermentation final product having both the effects of the salvia miltiorrhiza and the ganoderma fungi is obtained after bidirectional solid fermentation including influence of medical raw materials on the growth and metabolism process of ganoderma mycelium and transformation of a ganoderma fungal enzyme system on the medical materials compositions, and the invention can be applied to preparing a medicament and / or a cosmetic with the effects of promoting blood circulation by removing blood stasis and antioxidation.

Description

technical field [0001] The invention relates to a fungus substance mixture obtained by solid fermentation of fungi, a preparation method and application thereof. Specifically, it is a fungal substance mixture obtained by solid fermentation of ganoderma lucidum with a culture substrate containing medicinal ingredients, its preparation method and application. Background technique [0002] There have been many reports on the research on the metabolites of various medicinal (food) fungi, especially the anti-tumor research on polysaccharides. For a long time, its production research has been mainly focused on liquid submerged fermentation, mainly referring to the antibiotic production process; its production efficiency is high, and the product quality is relatively stable, but there are problems such as high cost, large energy consumption, easy to be polluted, and difficult to operate. [0003] The medium used in the solid fermentation production process mostly uses agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074A61K8/99A61K36/537A61Q19/00A61P39/06A23L1/28C12N1/14A23L31/00
Inventor 郑林用罗霞许晓燕江南余梦瑶曾瑾
Owner 成都宇泽生物基因化妆品有限公司
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