Application of evodiamine in preparation of preparation for resisting white spot syndromevirus
A technology for vitiligo syndrome and evodiamine, which is applied to the application field of evodiamine in the preparation of preparations against white spot syndrome in shrimp, can solve problems such as drug application limitation, inability to analyze, and ineffective solution, and achieves reduction of lethality, enhancement of The effect of resistance
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[0023] 1. Verification of the binding ability of Evodiamine and PjCaspase:
[0024] 1.1 Amplify the PjCaspase gene.
[0025] 1.1.1 cDNA synthesis of shrimp tissue:
[0026] 1.1.1.1 Extraction of total RNA from shrimp tissue:
[0027] 1) Take 200 mg of live shrimp tissue (Penaeus japonicus, purchased from a vegetable market in Hangzhou, Zhejiang Province), and grind it rapidly in 800 μL Trizol (BBI company).
[0028] 2) Place on ice for 5 minutes.
[0029] 3) Add 200 μL of phenol / chloroform solution (phenol and chloroform are mixed in equal volumes and then extracted several times with 0.1 mol / LTris.HCl (pH 7.6) to balance the mixture. mol / l Tris.HCl (pH7.6) liquid layer, stored at 4°C; use a pipette to absorb the lower layer solution), shake and mix, and then centrifuge at 12,000rpm for 10min.
[0030] 4) Pipette the supernatant to an equal volume of isopropanol.
[0031] 5) After shaking and mixing, centrifuge at 12,000 rpm for 10 minutes.
[0032] 6) The precipitate wa...
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