Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus

A technology for interleukin and leukoplakia syndrome, applied in antiviral agents, peptide/protein components, medical preparations containing active ingredients, etc., can solve problems such as drug application restrictions, inability to analyze, and unanswered questions. Achieve the effect of reducing the fatality rate and enhancing the resistance

Inactive Publication Date: 2013-07-17
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Shrimp is one of the most important mariculture products, but since the 1990s, it has been seriously threatened by diseases, especially white spot syndrome virus (white spot syndrome virus, WSSV) is the most important pathogen that harms cultured shrimp. It has strong ability and wide spread, and has caused huge losses to the world's shrimp farming industry. However, there is still no effective control method so far.
[0004] Many studies on shrimp diseases are only at the application level, and have not conducted a more in-depth discussion on the disease resistance mechanism of the obtained drugs, so the application of the drugs has been limited
At the same time, due to the inability to analyze its mechanism of action, the problems generated during the use of these drugs cannot be answered, and thus cannot be effectively resolved.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus
  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus
  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Verification of the binding ability of IL-2 and PjCaspase:

[0021] 1.1 Amplify the PjCaspase gene.

[0022] 1.1.1 cDNA synthesis of shrimp tissue:

[0023] 1.1.1.1 Extraction of total RNA from shrimp tissue:

[0024] 1) Take 200 mg of fresh live prawn tissue (Penaeus nigra, purchased from a vegetable market in Hangzhou, Zhejiang Province), and quickly grind it in 800 μL Trizol (BBI company).

[0025] 2) Put it on ice for 5 minutes.

[0026] 3) Add 200μL of phenol / chloroform solution (mix equal volumes of phenol and chloroform and extract several times with 0.1mol / LTris.HCl (pH 7.6) to balance the mixture. Place it in a brown glass bottle and cover it with an equal volume of 0.01 mol / l Tris.HCl (pH 7.6) liquid layer, store at 4°C; use a pipette to draw the lower layer solution), shake and mix well and centrifuge at 12,000 rpm for 10 min.

[0027] 4) Pipette the supernatant to the same amount of isopropanol.

[0028] 5) After shaking and mixing, centrifuge at 12,000 rpm for 10 mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of interleukin 2 in preparing a preparation for resisting prawn white spot syndrome virus. The interleukin 2 can be acted a prawn apoptosis pathway key protein and can be used for preparing a preparation of resisting the prawn white spot syndrome virus, can greatly strengthen the resistance of the prawn to the prawn white spot syndrome virus (WSSV) and reduce the lethallty rate. The invention has the main benefits that the interleukin 2 can effectively induce the activation of PjCcaspase protein, greatly strengthen the resistance of the prawn to the prawn WSSV and reduce the lethallty rate. Because the interleukin 2 is a drug widely applied to the human body for improving the immunity and has no toxicity to the human body, the prawn using the interleukin2 can be taken whether drug metabolism is complete or not.

Description

(1) Technical field [0001] The invention relates to the application of Interleukin-2 (Interleukin-2) in the preparation of preparations against prawn white spot syndrome. (2) Background technology [0002] Prawns are the most predominant crustaceans in tropical and subtropical shallow waters. Because of its variety, large population, strong fecundity, rapid growth, and delicious meat, it has high economic value. Due to environmental pollution, aquaculture technology, disease control and other reasons, prawns, especially Penaeus monodon, Penaeus vannamei, Penaeuschinensis and Marsupenaeus japonicus, are very easy Affected by the virus, and caused a lot of losses. White spot syndrome virus (WSSV) is the most damaging pathogen in shrimp farming. Making full use of the innate immune system and immune mechanism of prawns and stimulating the disease resistance mechanism of prawns to resist viruses is of great significance for the prevention and treatment of prawn diseases and improv...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/20A61P31/20
Inventor 章晓波智斌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com