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Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus

A technology for interleukin and vitiligo syndrome, which can be used in antiviral agents, peptide/protein components, and medical preparations containing active ingredients, etc. It can solve problems such as drug application limitations, inability to analyze, and no drug resistance mechanism research. , to reduce the fatality rate and enhance the resistance

Inactive Publication Date: 2010-10-20
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Shrimp is one of the most important mariculture products, but since the 1990s, it has been seriously threatened by diseases, especially white spot syndrome virus (white spot syndrome virus, WSSV) is the most important pathogen that harms cultured shrimp. It has strong ability and wide spread, and has caused huge losses to the world's shrimp farming industry. However, there is still no effective control method so far.
[0004] Many studies on shrimp diseases are only at the application level, and have not conducted a more in-depth discussion on the disease resistance mechanism of the obtained drugs, so the application of the drugs has been limited
At the same time, due to the inability to analyze its mechanism of action, the problems generated during the use of these drugs cannot be answered, and thus cannot be effectively resolved.

Method used

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  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus
  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus
  • Application of interleukin 2 in preparing preparation for resisting prawn white spot syndrome virus

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Embodiment 1

[0020] 1. Verification of the binding ability of IL-2 and PjCaspase:

[0021] 1.1 Amplify the PjCaspase gene.

[0022] 1.1.1 Shrimp tissue cDNA synthesis:

[0023] 1.1.1.1 Extraction of total RNA from shrimp tissue:

[0024] 1) Take 200 mg of fresh prawn tissue (Japanese prawn, purchased from a vegetable market in Hangzhou City, Zhejiang Province), and quickly grind it in 800 μL Trizol (BBI Company).

[0025] 2) Put on ice for 5 minutes.

[0026] 3) Add 200 μL of phenol / chloroform solution (the phenol and chloroform are mixed in equal volumes and then extracted several times with 0.1 mol / LTris.HCl (pH 7.6) to equilibrate the mixture, put it in a brown glass bottle, and cover it with an equal volume of 0.01 mol / l Tris.HCl (pH 7.6) liquid layer, stored at 4°C; use a pipette to suck the lower layer solution), shake and mix, and then centrifuge at 12,000 rpm for 10 min.

[0027] 4) Pipette the supernatant to the same amount of isopropanol.

[0028] 5) After shaking and mixing...

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Abstract

The invention provides application of interleukin 2 in preparing a preparation for resisting prawn white spot syndrome virus. The interleukin 2 can be acted a prawn apoptosis pathway key protein and can be used for preparing a preparation of resisting the prawn white spot syndrome virus, can greatly strengthen the resistance of the prawn to the prawn white spot syndrome virus (WSSV) and reduce the lethallty rate. The invention has the main benefits that the interleukin 2 can effectively induce the activation of PjCcaspase protein, greatly strengthen the resistance of the prawn to the prawn WSSV and reduce the lethallty rate. Because the interleukin 2 is a drug widely applied to the human body for improving the immunity and has no toxicity to the human body, the prawn using the interleukin2 can be taken whether drug metabolism is complete or not.

Description

(1) Technical field [0001] The present invention relates to the application of interleukin-2 (Interleukin-2) in the preparation of preparations against white spot syndrome of shrimp. (2) Background technology [0002] Shrimp is the most dominant crustacean in shallow tropical and subtropical seas. Because of its many species, large population, strong fecundity, rapid growth and delicious meat, it has high economic value. Due to environmental pollution, aquaculture technology, disease control and other reasons, shrimp, especially Penaeus monodon (Penaeus monodon), Penaeus vannamei (Penaeus vannamei), Chinese shrimp (Penaeuschinensis) and Japanese shrimp (Marsupenaeus japonicus), are easily Infected by the virus, and caused a lot of losses. White spot syndrome virus (WSSV) is the most serious pathogen in shrimp farming. Making full use of the innate immune system and immune mechanism of prawns to resist viruses by stimulating the disease resistance mechanism of prawns is of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61P31/20
Inventor 章晓波智斌
Owner ZHEJIANG UNIV
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