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Process for preparing collagen tripeptide

A collagen tripeptide, technology, applied in skin care preparations, cosmetics, fermentation and other directions, to achieve the effect of broad market prospects

Active Publication Date: 2013-11-20
武汉天天好生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, further research is needed on the extraction of fish skin collagen

Method used

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  • Process for preparing collagen tripeptide
  • Process for preparing collagen tripeptide
  • Process for preparing collagen tripeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Feeding 50kg of lye (1.0% calcium hydroxide) and soaking the tilapia skins cleaned and drained for 1 day, adding 250L of water, boiling and denaturing for 30min, filtering the filtrate through gauze, cooling to about 50°C, adding 0.25kg of alkaline protease, Hydrolyze at 55°C for 1 hour, add 0.25 kg of neutral protease, hydrolyze for 1 hour, add 0.25 kg of compound protease, hydrolyze for 1 hour, steam inactivate the enzyme for 10 minutes; collect about 270 L of filtrate by filter cloth, pass through a carbon column, and finally concentrate and spray dry to obtain collagen III Peptide 5.1 kg.

[0047] Prepared collagen tripeptide product index is as follows:

[0048] Molecular weight range

Embodiment 2

[0050]Feeding 60kg of lye (1.0% calcium hydroxide) and soaking the tilapia skins cleaned and drained for 1 day, adding 360L of water, boiling and denaturing for 30min, collecting the filtrate by gauze filtration, cooling to about 60°C, adding 0.60kg of alkaline protease, Hydrolyze at 60°C for 1 hour, add 0.60 kg of neutral protease, hydrolyze for 1 hour, add 0.60 kg of compound protease, hydrolyze for 1 hour, steam inactivate the enzyme for 10 minutes; collect about 400 L of filtrate by filter cloth, pass through a carbon column, and finally concentrate and spray dry to obtain collagen III Peptide 6.3kg.

[0051] Prepared collagen tripeptide product index is as follows:

[0052] Molecular weight range

Embodiment 3

[0054] Feeding 55kg of lye (1.0% calcium hydroxide) soaked for 1 day to clean and drain the tilapia skin, add 330L of water, boil and denature for 35min, collect the filtrate by gauze filtration, cool to about 50°C, add 0.275kg of alkaline protease, Hydrolyze at 50°C for 1 hour, add 0.275 kg of neutral protease, hydrolyze for 1 hour, add 0.275 kg of compound protease, hydrolyze for 1 hour, steam inactivate the enzyme for 10 minutes; collect about 360 L of filtrate by filter cloth, pass through a carbon column, and finally concentrate and spray dry to obtain collagen III Peptide 5.7kg.

[0055] Prepared collagen tripeptide product index is as follows:

[0056] Molecular weight range

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Abstract

The invention provides a process for preparing collagen tripeptide. The process comprises the following steps that: performing alkali liquor pre-treatment and boiling denaturation treatment on fish skin, filtering residues by using gauze; performing fractional composite hydrolysis of bacillus subtilis alkali protease, neutral protease and composite protease to obtain hydrolysis liquid; and inactivating and filtering the hydrolysis liquid, debitterizing and decoloring the filtrate, performing vacuum concentration on the filtrate to obtain concentrated solution, and performing spray drying on the concentrated solution to obtain the collagen tripeptide with high content of protein and good functional activity. By adopting the fish skin as the raw material, the process increases raw material types of the collagen tripeptide, and the prepared collagen tripeptide has multiple functions, so the process has wide commercial prospect.

Description

technical field [0001] The invention relates to a process for preparing collagen tripeptide with multiple active functions. Background technique [0002] Collagen peptide is used as a raw material for cosmetics. It has good cell adhesion, absorption and biocompatibility. It is used as a moisturizer and skin protectant in cosmetics, which can promote the formation of epithelial cells, directly supplement the collagen lost by the human body, and delay skin aging. It can be used for anti-wrinkle, moisturizing and repairing, etc. [0003] The smallest unit of collagen is collagen tripeptide (CTP). Its structure can be expressed as (Gly-X-Y), which is a highly pure collagen tripeptide with glycine at the N-terminal. When applied to the surface of the skin, it exhibits excellent moisturizing effects and is non-allergenic. Microautoradiography studies have shown that CTP has an average molecular weight of about 280 Da. [0004] Collagen has long been mainly extracted from anima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A61Q19/00A61Q19/08
Inventor 陈栋梁
Owner 武汉天天好生物制品有限公司