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Multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in biological sample

A technology for biological samples and polychlorinated biphenyls, which is applied in the field of chromatography, can solve the problems such as rapid pretreatment and purification methods for polychlorinated biphenyl compounds that have not yet been found, and achieve the effects of low cost, high separation and purification efficiency, and low pollution.

Inactive Publication Date: 2010-10-27
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, no method has been found to realize the rapid pretreatment and purification of PCBs in biological samples by changing the multi-stage modification method of chromatographic column packing

Method used

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  • Multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in biological sample
  • Multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in biological sample

Examples

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Embodiment

[0027] Example: The composition and use of a multi-stage improved column for rapid pretreatment and purification of PCBs in biological samples

[0028] 1. The composition of multi-level improved columns

[0029] Such as figure 1 As shown, a glass material chromatographic column with an inner diameter of 28 mm and a length of 100 mm is taken as an example to illustrate the composition of the multi-stage improved column of the present invention, which includes the elution and outflow part of the conventional chromatographic column, the column packing part and the pressure part of the sample. The column body of the column packing part includes four parts packed with different packings in sequence, of which:

[0030] Part I in the column belongs to the neutral chromatography part, accounting for 20% of the entire column, and the filler is 200 mesh neutral silica gel;

[0031] The second part of the column belongs to the neutral degreasing part, which accounts for 20% of the entire column....

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Abstract

The invention discloses a multistage improvement column for quickly pre-processing and purifying polychlorinated biphenyl in a biological sample. The multistage improvement column comprises an eluting and outflowing part, a column filler and a pressing part for feeding a sample. The multistage improvement column is characterized in that the column filler consists of four parts, wherein a part I in a column body is a neutral chromatography part which account for 20 percent of the total column body, and the filler is neutral silica gel; a part II in the column body is a neutral degreasing part which accounts for 20 percent of the total column body, and the filler is selected according to specific biomolecules; a part III in the column body is an alkali modified part which accounts for 30 percent of the total column body, and the filler is alkaline aluminum hydroxide, Florisil or a mixed material of magnesium oxide, aluminum oxide and the like; and a part IV in the column body is an acidmodified part which accounts for 20 to 30 percent of the total column body, and the filler mainly comprises 3-6N strong acid modified silica gel. The multistage improvement column for quickly pre-processing and purifying the polychlorinated biphenyl in the biological sample has the advantages of high efficiency of separation and purification, low pollution, low requirements on instruments and equipment, low cost and the like, and the multistage improvement column is suitable for large-area popularization and application.

Description

Technical field [0001] The invention relates to the technical field of chromatography, in particular to an improved column composed of a novel column packing, which can be used for rapid pretreatment and purification of polychlorinated biphenyl compounds in complex biological samples. Background technique [0002] Polychlorinated biphenyl organic pollutants are one of the common environmental pollutants. They are usually synthesized by the chemical industry and released through different pathways or into different environmental media. Most of these compounds are hydrophobic and lipophilic substances, so they can be enriched in biological fats. In 1968, a well-known pollution disease occurred in Japan due to PCB contamination of rice bran oil. Although countries began to reduce or stop production after 1973, they are still widely present in different environmental media, enter organisms through different exposure pathways, accumulate in the adipose tissues and organs of humans an...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/60
Inventor 王红梅刘茜
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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