Application of ligusticum chuanxiong hort or extract thereof in preparing anti-aging products
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Rhizoma Chuanxiong extract and water extract, which is applied in the application field of Rhizoma Chuanxiong or its extract in the preparation of anti-aging products, which can solve the problems that the research on anti-aging effect has not been reported, and achieve the effect of prolonging life
Active Publication Date: 2012-04-18
EXPERIMENTAL RES CENT CHINA ACAD OF CHINESE MEDICAL SCI
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
Although Chuanxiong is widely used in the treatment of senile diseases and has achieved good curative effect, there is no report on its anti-aging effect
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0023] Embodiment 1, Chuanxiong extract on the impact test of Caenorhabditis elegans lifespan
[0024] 1 material
[0025] 1.1 strains, nematodes
[0026] Strain E.coli OP50, wild-type Caenorhabditis elegans Bristol (N 2 ) were purchased from the Nematode Genetics Center (CGC).
[0027] 1.2 Drugs and reagents
[0028] Ligusticum chuanxiong extract is prepared according to the following method: select pieces of medicinal material Ligusticum chuanxiong (dried rhizome of Umbelliferae Ligusticumchuanxiong Hort.), add 6 times pure water, heat, boil and keep boiling for 40 minutes, then filter Collect filtrate 1; then add 6 times of pure water to the filter residue, heat, keep boiling for 40 minutes after boiling, then filter and collect filtrate 2; mix filtrate 1 and filtrate 2, and dry the obtained powder, which is Chuanxiong Water Extract. According to the method, 1 gram of Chuanxiong water extract is prepared from 20 grams of crude drug.
[0029] Agar powder (Japan Kyowa C...
Embodiment 2
[0049] Example 2, Molecular Mechanism of Ligusticum Chuanxiong Extract Prolonging the Lifespan of Caenorhabditis elegans
[0050] 1 material, instrument are with embodiment 1.
[0051] 2 Grouping of real-time fluorescence quantitative PCR (qRT-PCR) experiment: control group and medication group (the group with the strongest life-prolonging effect in life-span experiment, CXE solution with a concentration of 25 mg / L).
[0052] 3 Research on the molecular mechanism of life extension
[0053] 3.1 Extraction of nematode total RNA and cDNA synthesis
[0054] About 1,000 nematodes in the L4 stage were picked from the control group and the treatment group into the culture medium. After feeding for 10 days, the nematodes in each group were collected separately, and the total RNA was extracted by TRIZOL method, digested with RNase free DNase, and purified by TRIZOL method. Gel electrophoresis identified that the RNA was not significantly degraded, and was frozen at -80°C. Take 2 μg ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a new application of ligusticum chuanxiong hort or an extract thereof. The ligusticum chuanxiong hort or the extract thereof has the following four new purposes: (1) application in preparing anti-aging products; (2) application in preparing gene expression products for regulating caloric restriction pathways; (3) application in preparing gene expression products for regulating insulin signaling pathways; and (4) application in preparing caenorhabditis elegans life extenders. In the invention, the caenorhabditis elegans is used as a model organism, the influence of CXE (Ligusticum Chuangxiong Extract) on caenorhabditis elegan life is observed by taking a whole animal experiment as a detection means, the CXEs of a plurality of concentrations are all found to have a role or trend of life prolongation to the caenorhabditis elegan, wherein the concentration of 25mg / L has an optimal life prolongation effect, and the average life and the longest life are both prolonged by about 15 percent. Molecular mechanism research for life prolongation by utilizing the known relevant caenorhabditis elegan aging gene and adopting a qRT-PCR (Quantitative Real Time-Polymerase Chain Reaction) method shows that the CXE prolongs the life through intervening an Insulin / IGF-1 pathway and a feeding control (caloric restriction) pathway, thereby providing new content and direction for the molecular mechanism research of the drug and also providing a new ideal and means for the research of delaying aging by using the traditional Chinese medicine.
Description
technical field [0001] The invention relates to the new application of Rhizoma Chuanxiong or its extract for antiaging, in particular to the application of Rhizoma Chuanxiong or its extract in preparing antiaging products. Background technique [0002] With the increasingly severe situation of population aging, research on aging mechanism and anti-aging drugs has become a hot spot and key issue in life sciences. The mechanism of aging is complex and there are many theories. So far, there are more than 200 theories or theories explaining the mechanism of aging, including: genetic control theory, cell telomere theory, free radical damage theory, somatic cell mutation theory, immune disorder theory, Mitochondrial damage theory, etc. . Among them, there are two main ways to delay aging, one is the insulin signaling pathway (AntebiA. Inside insulin signaling, communication is key to long life [J]. SciAgingKnowledge environ, 2004, (23): pe25); Caloric restriction pathway (Koen ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more