Immunochromatography test strip for detecting cystic echinococcosis and preparation method thereof

A cystic hydatid disease and immunochromatography technology, which is applied to measurement devices, analytical materials, instruments, etc., can solve the problems of high requirements, time-consuming and laborious, and is not suitable for on-site use, and achieves a simple detection method and broad application prospects. , the effect of low cost

Inactive Publication Date: 2010-11-10
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are time-consuming and laborious, or require a cold chain system to preserve reagents, or have high requirements for equipment and operators, and are not suitable for on-site use.

Method used

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  • Immunochromatography test strip for detecting cystic echinococcosis and preparation method thereof
  • Immunochromatography test strip for detecting cystic echinococcosis and preparation method thereof
  • Immunochromatography test strip for detecting cystic echinococcosis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the analysis of the response analysis of the serum of the echinococcosis patient to the crude antigen and the analysis of the dominant specific antibody subclass

[0054] 1. Preparation of natural antigen against cystic echinococcosis

[0055] Take the fresh goat liver encapsulation fluid, centrifuge at 3000r / min for 20min, suck the supernatant into the dialysis bag, dialyze with PBS solution at 4°C overnight, centrifuge the dialyzed encapsulation fluid at 6000r / min for 20min, take the supernatant, The precipitate was discarded, and the crude antigen of the cystic fluid was obtained, which was freeze-dried and stored at low temperature for later use.

[0056] 2. Method: ELISA method was used for analysis, and the crude cystic fluid antigen was used as the coated antigen to react with the serum of echinococcosis patients, non-echinococcosis patients and healthy people respectively, and then the goat anti-human IgG1 antibody horseradish peroxide Enzyme conj...

Embodiment 2

[0060] Embodiment 2, the preparation of the immunochromatography test strip that detects cystic echinococcosis

[0061] 1. The preparation of the natural antigen against cystic echinococcosis is the same as in Example 1.

[0062] 2. Preparation of monoclonal antibodies against dominant specific antibody subclasses of hydatid antigens

[0063] BALB / c mice were immunized with human IgG4 antigen according to the conventional method, splenocytes from the immunized mice were fused with SP2 / 0 tumor cells, and the hybridoma cells with high function and high efficiency secreting specific antibodies were cloned three times and then injected into mice to produce ascites. The obtained monoclonal antibody was purified by the conventional saturated ammonium sulfate salting-out method, and the antibody titer was detected by the immunoagar diffusion method. The results showed that the antibody titer was greater than 1:128, indicating that the obtained monoclonal antibody had good specificity...

Embodiment 3

[0081] Embodiment 3, the preparation of the immunochromatographic test strip that detects cystic echinococcosis

[0082] A colloidal gold-labeled antibody was prepared as a detection probe by using a mouse anti-human IgG4 monoclonal antibody purchased from BD Company of the United States (Product No. 555881), and other steps were the same as in Example 2.

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Abstract

The invention discloses an immunochromatography test strip for detecting cystic echinococcosis and a preparation method thereof. The test strip comprises a hemofiltration membrane sample pad, a gold-marking pad, a cellulose membrane and a water absorption pad, wherein the gold-marking pad is tightly connected with the hemofiltration membrane sample pad and comprises an antibody labeling colloid gold probe of anti-echinococcus antigen advantage special antibody subtypes, the cellulose membrane is tightly connected with the gold-marking pad, the water absorption pad is tightly connected with the other end of the cellulose membrane, a detection line and a quality control line are arranged on the cellulose membrane, the detection line comprises crude antigens aiming at the cystic echinococcosis, and the quality control line comprises anti-antibodies capable of realizing special combination with the antibodies of the anti-echinococcus antigen advantage special antibody subtypes. The immunochromatography test strip of the invention has the advantages of simplicity, convenience, sensitivity, specificity and high speed, and is applicable to clinical and field use.

Description

technical field [0001] The invention relates to the field of parasitic disease detection, in particular to a detection test strip for cystic echinococcosis and a preparation method thereof. Background technique [0002] Echinococcosis, also known as Hydatid disease or Hydatidosis, is a zoonotic parasitic disease caused by the larvae of Echinococcus tapeworms parasitic in humans and animals, and can infect human echinococcus There are four species of tapeworms: Echinococcus granulosus (Eg), Echinococcus multilocularis (Em), Echinococcus oligarthrus (E. ). The adult and larval stages of these four Echinococcus species are different in morphology and can cause different types of echinococcosis. There are two types of echinococcosis in my country, namely, cystic echinococcus-cosis (CE), also known as cystic echinococcosis, and alveolar echinococcosis (AE), also known as alveolar echinococcosis. sick. [0003] Echinococcosis is an important worldwide public health problem, wid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/552
Inventor 高春花汪俊云杨玥涛石锋朱慧慧王立英
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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