Method for detecting testosterone by using surface plasmon resonance (SPR) sensor

A surface plasmon and resonance sensor technology, which is applied in the measurement of phase influence characteristics, etc., can solve the problems of limiting the application of surface plasmon resonance technology, poor chip reuse performance, and harsh detection environment requirements, so as to reduce detection costs and selectivity. High, responsive effects

Inactive Publication Date: 2010-11-17
BEIJING INSTITUTE OF TECHNOLOGYGY
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Problems solved by technology

The research on immunoassay is one of the fields with the earliest application time, the widest application range and the most complete development of surface plasmon resonance sensing technology. However,

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  • Method for detecting testosterone by using surface plasmon resonance (SPR) sensor
  • Method for detecting testosterone by using surface plasmon resonance (SPR) sensor

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Embodiment 1

[0032]A surface plasmon resonance sensor chip, such as figure 1 As shown, it includes a glass substrate 1, a gold film layer 2, a monomolecular alkyl chain layer 3 and a molecularly imprinted polymer film 4; Base chain layer 3 and molecularly imprinted polymer film 4;

[0033] The structural formula of the monomolecular alkyl chain layer 3 is Au-(CH 2 ) 11 CH 3 ;

[0034] The molecularly imprinted polymer film 4 is obtained by photopolymerization of template molecules, functional monomers, crosslinking agents, pore forming agents and photoinitiators, the template molecule is the target molecule testosterone to be imprinted, and the functional monomer is methacrylic acid The crosslinking agent is ethylene glycol dimethacrylate; the pore forming agent is acetonitrile;

[0035] A method for preparing a surface plasmon resonance sensor chip, the specific steps of which are:

[0036] 1) The method of vacuum evaporation is used to plate a gold film layer 2 on the glass substra...

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Abstract

The invention relates to a method for detecting testosterone by using a surface plasmon resonance (SPR) sensor and belongs to the technical field of hormone detection and analysis. The method comprises the following steps of: fixing a testosterone molecular imprinted identification sensitive chip in the SPR sensor, performing angle scanning on the chip, and then recording an SPR spectrogram to obtain a resonance angle theta1; recording the SPR spectrogram to obtain the resonance angle theta n corresponding to each standard solution; drawing a standard curve by taking delta theta=theta n-theta1 as a vertical coordinate and taking the concentration of the testosterone standard solution as a horizontal coordinate; and injecting sample solution to be detected into a flow cell, and recording the SPR spectrogram to obtain the resonance angle theta x, and computing delta theta x=theta x-theta1 to obtain the testosterone content according to the delta theta x on the standard curve. The method has the advantages of quick response and high selectivity, wherein the result can be obtained in 5 to 10 minutes generally, and the super low testosterone content can be measured; and the chip of the sensor can be recycled, and the detection cost is reduced.

Description

technical field [0001] The invention relates to a method for detecting testosterone by using a surface plasmon resonance (Surface Plasmon Resonance, SPR for short) sensor, belonging to the technical field of hormone detection and analysis. Background technique [0002] Testosterone is a steroid hormone. Accurate detection of its content is of great significance and role in environmental monitoring, food safety and other aspects. [0003] The methods currently used in the detection of testosterone mainly include gas chromatography / mass spectrometry, isotope dilution mass spectrometry, thin-layer chromatography, chemiluminescence, high performance liquid chromatography, enzyme-linked immunoassay, radioimmunoassay, etc. Gas phase, liquid chromatography, and mass spectrometry are expensive and cumbersome to operate, while thin-layer chromatography has low sensitivity and is not suitable for quantitative analysis. Therefore, the detection of testosterone mostly adopts the radioi...

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Application Information

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IPC IPC(8): G01N21/41
Inventor 韦天新魏清泉王洋
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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