Method for purifying lutein esters

A technology for lutein esters and esters, which is applied in the field of extracting high-purity lutein esters, can solve problems such as low yield, influence on product biological activity, and failure to meet high content requirements, and achieve the effect of improving purity

A technology for lutein esters and esters, which is applied in the field of extracting high-purity lutein esters, can solve problems such as low yield, influence on product biological activity, and failure to meet high content requirements, and achieve the effect of improving purity

CN101891664AInactive Publication Date: 2010-11-24YUNNAN RAINBOW BIO TECH
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  • Method for purifying lutein esters

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Weigh 101g marigold ointment (total lutein ester content is 29.3%) and mix with 404ml food grade ethyl acetate, dissolve by heating at 50℃, keep stirring for 3 hours, then hot filter the solution at 70℃ to remove insoluble matter , Slowly add 505ml of food grade ethanol, stir for 2.5 hours, let stand at 5℃ for 16 hours, filter the precipitated solid crystals, and finally wash the crystals with 300ml of n-butanol. The solid crystals were dried in a vacuum drying oven at a drying temperature of 40°C and a vacuum of 0.075 MPa, and finally 26.0 g of dried solid matter was obtained. The content was 78.6% by UV detection. The yield of total lutein esters was 69.1%. .

Embodiment 2

[0029] Weigh 107g marigold ointment (total lutein ester content is 29.3%) and mix with 428ml food grade ethyl acetate, heat to dissolve at 50℃, keep stirring for 3 hours, then hot filter the solution at 70℃ to remove insoluble matter , Slowly add 535ml of food grade ethanol, stir for 2.5 hours, let stand at 5°C for 16 hours, filter the precipitated solid crystals, and finally wash the crystals with 300ml of n-propanol. The solid crystals were dried in a vacuum drying oven at a drying temperature of 40° C. and a vacuum of 0.075 MPa. Finally, 21.3 g of crystals were obtained, the lutein ester content was 74.2%, and the total lutein yield was 50.4%.

Embodiment 3

[0031] Weigh 103g marigold ointment (total lutein ester content is 29.3%) and mix with 412ml food grade ethyl acetate, dissolve by heating at 50℃, keep stirring for 3 hours, then hot filter the solution at 70℃ to remove insoluble matter , Slowly add 515ml of food grade ethanol, stir for 2.5 hours, and then stand at 5°C for 16 hours, filter the precipitated solid crystals, and finally wash the crystals with 300ml of ethanol. The solid crystals were dried in a vacuum drying oven at a drying temperature of 40° C. and dried under a vacuum of 0.075 MPa. Finally, 19.3 g of crystals were obtained, the lutein ester content was 71.7%, and the total lutein yield was 45.8%.

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Abstract

The invention discloses a method for purifying lutein esters and in particular relates to a method for extracting high-purity lutein esters from marigold ointment. The method is characterized by dissolving the marigold ointment into ethyl acetate at 25-80 DEG C and stirring the mixture for 1-6h; adding absolute ethyl alcohol to the liquid obtained through heat filtration and stirring the mixture at 20-70 DEG C for 0.5-5.0h to form uniform solution and then filtering the solution after cooling the solution at minus 5-30 DEG C for 5-20h; and then washing filter cakes with lower alcohol and drying the solid under vacuum to remove the solvent residues, thus obtaining the required high-purity lutein esters. The content of the lutein esters is 70-80%. The method is high in product yield, simple and convenient to operate and easy to realize large-scale industrial production.

Description

Technical field [0001] The present invention relates to a method for purifying lutein esters. More specifically, the present invention relates to a method for extracting high-purity lutein esters from marigold ointment. Background technique [0002] So far, there have been many reports on the extraction of lutein esters from marigold oleoresin. For example, US 4,048,203 uses hot alkanol containing no more than 4 carbon atoms to dissolve marigold oleoresin. The lutein ester is precipitated by cooling the solution, filtered to remove the alkanol and then vacuum dried, but the lutein obtained by this method The ester content is only 51%, which does not meet the high content requirement. In US6,191,293, the marigold flower is extracted with a hydrocarbon solvent and then with alcohol precipitation to obtain an extract, but the purity of the lutein ester obtained by this method varies greatly due to the quality of the raw materials, and it is difficult to control in industrial produc...

Claims

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Application Information

Patent Timeline
24 Nov 2010
Publication
CN101891664A
IPC
C07C403/24; C09B61/00; A23L1/275; A23L1/30; A23L5/43
Inventors
代刚; 苏记