Method for preparing fluorescence-encoded micro-beads and product thereof

A technology of fluorescent coding and microspheres, which is applied in the field of preparation of fluorescent coding microspheres, can solve the problems of easy loss of fluorescent substances, single fluorescent color of microspheres, and unsuitability for high-throughput analysis of biological samples, so as to avoid the loss of fluorescein. Liveness, uniform microsphere particle size, and the effect of preventing the loss of fluorescent substances

Inactive Publication Date: 2010-12-08
吕军
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing fluorescently coded microspheres and its products, which can solve the problem that fluorescent substances are easily lost during the preparation process of currently existing fluorescently coded microspheres, and the prepared microspheres have a single fluorescent color and are not suitable for biological applications. Disadvantages such as high-throughput analysis requirements for samples

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  • Method for preparing fluorescence-encoded micro-beads and product thereof
  • Method for preparing fluorescence-encoded micro-beads and product thereof

Examples

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Embodiment Construction

[0016] The preparation method of the fluorescence-encoded microspheres of the embodiment comprises the following steps:

[0017] (1) Wash the styrene monomer material with strong alkali (NaOH) for 3 times, and then wash it with pure water for 2 times;

[0018] (2) Add azobisisobutylcyanide, pyridine orange, rhodamine, lauryl glycine, and the final concentration of pyridine orange and rhodamine is 5%;

[0019] (3) Add ethanol, isopropanol, and polyvinylpyrrolidone, then use a vacuum pump to evacuate, then fill with nitrogen, adjust the temperature of the water bath to about 500°C, and carry out the polymerization reaction;

[0020] (4) adding α-methacrylic acid to modify the fluorescently encoded microspheres with carboxyl functional groups;

[0021] (5) Fluorescence-encoded microspheres were washed with absolute ethanol.

[0022] The product prepared by the method for preparing fluorescently encoded microspheres described in the examples includes spheres 1 and functional gro...

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Abstract

The invention discloses a method for preparing fluorescence-encoded micro-beads, which comprises the following steps of: cleaning styrene with alkali, and cleaning with pure water; adding azodiisobutyronitrile, a fluorescent material and a stabilizer; performing polymerization; performing functionalization modification and the like. The fluorescence-encoded micro-beads comprise beads and functional groups modified on the beads. The main components of the beads comprise the fluorescent material and styrene polymer, and the fluorescent material is dispersed in the styrene polymer. The method overcomes the defects that the conventional fluorescent material is easy to lose, and the prepared micro-beads have single fluorescent color, does not meet the high-flux analysis requirement of biological samples and the like.

Description

technical field [0001] The invention relates to a method for preparing fluorescently coded microspheres and products thereof. Background technique [0002] At present, most of the preparation methods of fluorescence-encoded microspheres are to first polymerize the microspheres by seed polymerization, then dissolve the water-insoluble organic fluorescent materials in water-soluble organic solvents such as acetone and alcohol, and then combine them with the water of the microspheres. When the dispersion system is mixed, the fluorescent material will be precipitated and adsorbed onto the microspheres. Although this method is simple, the adsorbed fluorescent substance is easy to lose, the toxicity is high, and the operation is cumbersome. The monodispersity of the prepared fluorescently encoded microspheres is not ideal, and the particle size is not uniform. [0003] The fluorescence-encoded microspheres prepared by the traditional method contain only one type of fluorescent su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F112/08C08F2/44C08K5/00C08K5/18C08K5/46C08K5/36C09K11/06B01J13/18G01N21/64
Inventor 吕军赵世鹏
Owner 吕军
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