Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing phytic acid micelle modified hydrogen peroxide sensor

A hydrogen peroxide and sensor technology, applied in the field of biosensors, can solve problems such as troublesome detection and low sensitivity

Inactive Publication Date: 2013-03-13
SHANGHAI NORMAL UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art are: troublesome detection and low sensitivity
[0004] So far, there are no examples of using phytic acid micelles to prepare biosensors for detecting hydrogen peroxide at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing phytic acid micelle modified hydrogen peroxide sensor
  • Method for preparing phytic acid micelle modified hydrogen peroxide sensor
  • Method for preparing phytic acid micelle modified hydrogen peroxide sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0044] The present invention will be further described below through specific embodiments.

[0045] Phytic acid based micellar spheres IP 6 The preparation method of the horseradish peroxidase HRP sensor prepared by micelles: the 0.001mol / L sodium phytate solution was heated to reflux at 90°C for 20 minutes, and then cooled at room temperature to self-assemble into phytic acid micelles. Pipette 5 μL of the above-mentioned phytic acid micellar solution and drop it on the surface of the polished glassy carbon electrode, and let it dry at room temperature. Then take 3 μL of 5 mg / mL horseradish peroxidase solution and drop-coat it on the glassy carbon electrode, and place it in a refrigerator at 4°C. After drying, 3 μL of perfluorosulfonic acid solution diluted five times with pH 7.0 phosphate buffer solution (stock concentration: 5%) was added dropwise, dried and stored in a refrigerator at 4°C.

[0046] The above descriptions are only preferred embodiments of the present inven...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to biosensor technology, and discloses a method for preparing a phytic acid micelle modified hydrogen peroxide sensor. The method for detecting H2O2 content in the prior art has the defects of troublesome detection and low sensitivity. The method comprises the following steps of: performing heating reflux on 0.001mol / L of sodium phytate solution for 20 minutes at the temperature of 90 DEG C, and cooling the solution at room temperature; dispensing 5muL of phytic acid micelle solution on the surface of a polished 7mm<2> glassy carbon electrode, and airing the glassy carbon electrode at room temperature; fixing 3muL of 5mg / mL horseradish peroxidase solution on the phytic acid micelle-glassy carbon electrode; putting the electrode in a 4DEG C refrigerator, and drying the electrode; and dripping 3muL of perfluorinated sulfonic acid diluent on the phytic acid micelle-glassy carbon electrode to obtain a phytic acid micelle horseradish peroxidase sensor. The preparation method has the advantages of simplicity, low cost and capabilities of directly realizing electron transfer and measuring hydrogen peroxide accurately; and the sensor has the advantages of low detection limit, high sensitivity and good biological affinity.

Description

technical field [0001] The invention belongs to biosensor technology, in particular to a preparation method of a hydrogen peroxide sensor modified by phytic acid micelles. Background technique [0002] Hydrogen peroxide H 2 o 2 It has various functions such as oxidation, bleaching, disinfection and sterilization; 2 o 2 The determination of content is of great significance in the fields of industrial analysis, environmental monitoring, biopharmaceuticals, food science and clinical chemistry. Existing H 2 o 2 The methods for content detection include titration, spectroscopy, fluorescence, chemiluminescence and electrochemical methods. The disadvantages of the prior art are: troublesome detection and low sensitivity. In recent years, electrochemical methods, especially direct electrochemical media-free biosensors based on enzymes, have been developed rapidly, because the electrochemically prepared H 2 o 2 The preparation process of the biosensor is simple and low in co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 杨海峰苗云
Owner SHANGHAI NORMAL UNIVERSITY