Preparation and application of coconut globulin ferrous complex iron supplement agent

A globulin and iron supplement technology, applied in anti-plant immunoglobulin, peptide/protein components, medical preparations containing active ingredients, etc., can solve the problems of underutilization and waste of protein resources, etc.

Inactive Publication Date: 2010-12-22
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in coconut processing, coconut protein is mainly used as a by-product of processing cocon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of coconut globulin: take a certain amount of defatted coconut bran, add distilled water according to 1:15 (g / ml), stir and extract at room temperature for 90min, filter, then add distilled water in the same proportion in coconut bran, So repeated extraction 3 times, remove coconut albumin. Add pH 8.0 phosphate buffer solution to the remaining coconut bran according to 1:15~1:25 (g / ml), stir and extract at room temperature for 90min, repeat the extraction for 3 times, filter, collect the filtrate, and put it at 4°C under 6000r / min, centrifuge for 15 min, collect the supernatant, and then adjust the pH of the supernatant to 3.5-4.0 to precipitate the isoelectric point of the globulin. The precipitate was collected, washed 3 times with distilled water, and vacuum freeze-dried to obtain coconut globulin.

[0018] (2) Complexation of ferrous ions: use physiological saline as a solvent to prepare a coconut globulin solution with a concentration of 100 μg / mL...

Embodiment 2

[0023] (1) Preparation of coconut globulin: take a certain amount of defatted coconut bran, add distilled water according to 1:25 (g / ml), stir and extract at room temperature for 120min, filter, then add distilled water in the same proportion in coconut bran, So repeated extraction 3 times, remove coconut albumin. Add pH 8.0 phosphate buffer solution to the remaining coconut bran according to 1:15 (g / ml), stir and extract at room temperature for 120 minutes, repeat the extraction 3 times, filter, collect the filtrate, and centrifuge at 6000r / min for 15 minutes at 4°C , collect the supernatant, and then adjust the pH of the supernatant to 3.5-4.0 to precipitate the isoelectric point of the globulin. The precipitate is collected, washed with distilled water for 3 to 5 times, and vacuum freeze-dried to obtain coconut globulin.

[0024] (2) Complexation of ferrous ions: use physiological saline as a solvent to prepare a coconut globulin solution with a concentration of 100 μg / mL,...

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PUM

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Abstract

The invention discloses the preparation and the application of a coconut globulin ferrous complex iron supplement agent, comprising the following steps of: preparing coconut globulin, complexing ferrous ions and purifying and drying a product. The coconut globulin ferrous complex iron supplement agent can be prepared into a coconut globulin iron supplement oral liquid and coconut globulin ferrous complex microcapsules. The coconut globulin ferrous complex iron supplement agent comprises 12.95-25.50 percent of iron and has no peculiar smell, and a solution thereof is clear and stable in a pH value of 5-10 with a hazel color and has the maximum water solubility in a pH value of 7.0. The coconut globulin ferrous complex iron supplement agent has a certain blood enrichment function for iron-deficiency anemia, is safe, has no toxic or side effects and has the health functions of oxidation resistance, cholesterol lowering, bacteriostasis, hyperlipemia inhabitation, and the like.

Description

technical field [0001] The invention relates to the technical field of post-production processing of agricultural products, in particular to an iron supplement prepared by using coconut globulin complexed with ferrous ions and its application. Background technique [0002] Iron deficiency anemia (iron deficiency anemia, IDA) refers to the anemia that occurs when the iron storage in the body cannot meet the needs of normal erythropoiesis. Too much. Iron deficiency anemia is extremely harmful to the body. Iron deficiency can significantly reduce children's intelligence and motor development, and reduce people's immune function, physical strength, and endurance; Infant mortality is associated; iron deficiency can also damage the digestive tract, hair, nails, and skin. In short, iron deficiency anemia is one of the four most serious nutritional diseases in the contemporary world, which seriously affects human health. According to statistics published by the World Health Organ...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61K39/395C07K14/415C07K16/16A61P3/02
Inventor 郑亚军唐敏敏赵松林李艳陈卫军夏秋瑜
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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