Bacillus subtilis and application thereof for degrading decabromodiphenyl oxide

A technology of Bacillus subtilis and decabromodiphenyl ether, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems that Bacillus subtilis degrades decabromodiphenyl ether, etc., and achieve high-efficiency secondary pollution, Low cost, no secondary pollution

Inactive Publication Date: 2010-12-22
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report on the ability of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Bacillus subtilis DB-2 debromination degradation test of decabromodiphenyl ether with a concentration of 10mg / L:

[0042] Degradation basal medium formula: Each liter of medium contains 1g of peptone, 0.5g of yeast extract, KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH 4 Cl1.0g, NaCl0.5g, MgSO 4 5g, CaCl 2 2H 2 O0.15g, the balance is water.

[0043] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0044] Add 1ml of 300mg / L decabromodiphenyl ether stock solution dissolved in dichloromethane to a 100ml conical flask, avoid light until the dichloromethane volatilizes, then add 30ml of degradation basal medium to the conical flask, shake well to obtain 30ml of degradative Experiment medium.

[0045] That is, the formula of the degradation experiment medium: each liter of medium contains 10 mg of decabromodiphenyl ether, 1 g of peptone, 0.5 g of yeast extract, and KH 2 PO 4 2.93g, Na 2 HPO 4 8.87g, NH 4 Cl1.0g, NaCl0.5g, MgSO ...

Embodiment 2

[0055] Bacillus subtilis DB-2 debromination degradation test of decabromodiphenyl ether with a concentration of 10mg / L:

[0056] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0057] The degradation test medium in this embodiment is the same as the degradation test medium in Example 1.

[0058] 2. Culture the Bacillus subtilis DB-2 pure bacteria of the present invention on LB plate, then pick a single colony from the solid LB plate and inoculate it into 30ml of degradation experiment medium, cultivate it at 30°C, 150 rpm, in the dark, and cultivate for 60h Finally, take the culture solution, utilize ion chromatography to analyze the bromide ion concentration in the culture solution, and do three parallel samples in the experiment.

[0059] 3. Determination of the degradation rate of Bacillus subtilis DB-2:

[0060] Take 2mL of the above-mentioned culture solution from three parallel samples, filter it with a 0.22μm sterile filter ...

Embodiment 3

[0062] Bacillus subtilis DB-2 debromination degradation test of decabromodiphenyl ether with a concentration of 10mg / L:

[0063] 1. Prepare a degradation experiment medium containing 10 mg / L of decabromodiphenyl ether:

[0064] The degradation test medium in this embodiment is the same as the degradation test medium in Example 1.

[0065] 2. Routinely culture the Bacillus subtilis DB-2 pure bacteria of the present invention on an LB plate, then pick a single bacterium from the solid LB plate and inoculate it into a 30ml degradation experiment medium, and cultivate it in the dark at 30°C and 150 rpm for 72 hours , take the culture solution, use ion chromatography to analyze the bromide ion concentration in the culture solution, and do three parallel samples in the experiment.

[0066] 3. Determination of the degradation rate of Bacillus subtilis DB-2:

[0067] Take 2mL of the above-mentioned culture solution from three parallel samples, filter it with a 0.22μm sterile filter ...

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Abstract

The invention discloses bacillus subtilis DB-2 and application thereof for degrading decabromodiphenyl oxide. The bacillus subtilis is preserved in China Center for Type Culture Collection (CCTCC) on 27th November 2009 with the preservation number of CCTCC No:M 209286. The bacillus subtilis has stronger degrading ability and is capable of debrominating decabromodiphenyl oxide to generate free bromonium ions. The bacillus subtilis does not generate high-toxicity secondary pollutants of polybrominated dibenzo-p-dioxins, polybrominated dibenzo-furans, low-brominated diphenyl ether, and the like after being biodegraded, and can be used for treating the pollution of the decabromodiphenyl oxide in the environment, thereby providing a decabromodiphenyl oxide degrading bacterium with low cost and high efficiency and without secondary pollution for treating the decabromodiphenyl oxide in chemical sludge or wastewater.

Description

Technical field: [0001] The invention belongs to the field of microorganism application, and in particular relates to a bacillus subtilis (Bacillus subtilis DB-2) and its application in degrading decabromodiphenyl ether. Background technique: [0002] Decabromodiphenyl ether (BDE2209 or DeBDE) is the most demanded polybrominated diphenyl ether in the market. As a kind of brominated flame retardants (BFRs), it is widely used as impact-resistant polystyrene, polyester , polyamide, textile and electronics additives. In recent years, the demand for decabromodiphenyl ether has continued to increase. According to statistics, in 1999, the demand for BDE2209 accounted for 81% of the total demand for polybrominated diphenyl ethers in the world, and in 2001 it was 83%. As of 2001, the total output of flame retardants in my country was about 1.5×10 5 t, while the sales volume of BDE2209 has reached 1.35×10 4 t. The content of decabromodiphenyl ether in the global natural environmen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/125A62D101/28
Inventor 邓代永许玫英郭俊孙国萍陈杏娟
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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