Method for purifying phage through chromatography
A purification method and phage technology, applied in the biological field, can solve the problems of no related research reports on phage purification, and achieve the effect of overcoming the poor curative effect of drugs and treating bacterial diseases
Inactive Publication Date: 2010-12-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Abstract
The invention relates to a method for purifying phage through chromatography, which comprises the following steps of: (1) amplifying the phage; (2) enriching the phage to obtain a phage suspension; and (3) performing column chromatography: performing column chromatography to the enriched phage to obtain the purified phage, wherein a column packing material for column chromatography is sepharose 4B gel or DEAE-52 resin. The purified phage can be applied to clinic treatment and can overcome the defect of poor drug curative effect caused by antibiotic abuse and production of pathogenic drug-resisting stains. Under the current situation that multiple drug-resisting stains widely exist and no effective treatment method is available, the phage can be used for cracking pathogenic bacteria and killing host cells, and thus, the purpose of treating bacterial disease is achieved.
Application Domain
Recovery/purification
Technology Topic
Multiple drug resistanceBacterial disease +12
Examples
- Experimental program(3)
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