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Method for producing pharmaceutical-grade glycosaminoglycan by biological fermentation process

A technology of glucuronic acid and biological fermentation method is applied in the field of production of mucopolysaccharide by biological fermentation method, which can solve the problems of low product extraction rate, high production cost, single raw material, etc., and achieves simple process, low production cost and improved preparation effect.

Inactive Publication Date: 2010-12-29
CHINA EASTAR HLDG GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a bio-fermentation method for the production of pharmaceutical-grade glucose in view of the shortcomings of the prior art that the product extraction rate is too low, and the purity is not high, and the raw material is single and limited, which leads to the production cost of the product being too high. Aldehyde mucopolysaccharide method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for producing pharmaceutical-grade glucuronic acid mucopolysaccharide by biological fermentation, the process steps comprising:

[0019] (1) According to the ratio of 0.5% of glucose, 0.5% of yeast powder, 0.02% of dipotassium hydrogen phosphate, 0.05% of magnesium sulfate, and 97.43% of water (mass percentage), they are mixed successively in a shaker flask, and the bacterial strains are batched; (2) After mixing The raw materials are placed in the door of the shaker, and the temperature is controlled at 37±0.5°C for constant temperature cultivation; (3) The shake flask strain is cultivated at 12±1.5°C; (4) Under the protection of the flame, the inoculation bottle is connected to the inoculation port, Use the pressure difference to insert the bacteria into the seed tank; (5) After the inoculation, adjust the tank pressure to 0.01-0.05MP, and keep the temperature in the tank at 37±0.5°C for cultivation; (6) The bacteria grow in the seed tank 12±0.5 hours, connec...

Embodiment 2

[0021] A method for producing medical-grade glucuronic acid mucopolysaccharides by biological fermentation, the process steps comprising: 5 g of glucose, 5 g of yeast powder, 0.2 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, and 974.38 g of water are mixed in a shaking flask in sequence, and the ingredients Bacteria; the mixed raw materials are placed on the door of the shaker, the temperature is controlled at 37±0.5°C, and the temperature is cultivated for 12 hours; under the protection of the flame, the inoculation bottle is connected to the inoculation port, and the bacteria are connected to the seed tank by using the pressure difference After the inoculation, adjust the pressure of the tank to 0.02MP, and keep the temperature in the tank at 37±0.5°C for cultivation; the bacteria grow in the seed tank for 12 hours, and then connect to the fermenter for fermentation. During fermentation, the pressure of the fermenter is controlled at 0.05 MP, the pH value o...

Embodiment 3

[0023] A method for producing medical-grade glucuronic acid mucopolysaccharide by biological fermentation, the process steps include: 4g of glucose, 6g of yeast powder, 0.18g of dipotassium hydrogen phosphate, 0.6g of magnesium sulfate, and 975g of water are mixed in a shaker flask in sequence, and the ingredients Seed; the mixed raw materials are placed on the door of the shaker, the temperature is controlled at 37±0.5°C, and the temperature is cultivated for 14 hours; under the protection of the flame, the inoculation bottle is connected to the inoculation port, and the strain is connected to the seed tank by using the pressure difference; After the inoculation, adjust the pressure of the tank to 0.02MP, and keep the temperature in the tank at 37±0.5°C for cultivation; the strain grows in the seed tank for 12 hours, then connects to the fermenter for fermentation, and the pressure of the fermenter is controlled at 0.05MP during fermentation , the fermentation pH value is main...

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Abstract

The invention provides a method for producing pharmaceutical-grade glycosaminoglycan by a biological fermentation process. The method comprises the following steps of: sequentially mixing 0.5 mass percent of glucose, 0.5 mass percent of yeast powder, 0.02 mass percent of potassium acid phosphate, 0.05 mass percent of magnesium sulfate and 97.43 mass percent of water in a shake flask to prepare a strain; placing the mixture in a shaking table, controlling the temperature at 37+ / -0.5 DEG C and culturing the mixture at a constant temperature; inoculating the strain into a seeding tank by means of pressure difference under the protection of flames; inoculating the strain into a fermentation tank for fermentation after the inoculation and fully turning the strain; and performing emulsification, centrifugal separation and vacuum drying to obtain the glycosaminoglycan. The method has the characteristics of low production cost, simple process and the like.

Description

Technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method for producing mucopolysaccharide by biological fermentation. Background technique [0002] At present, there are two main production processes for producing glucuronic acid mucopolysaccharide at home and abroad, and the technical means adopted are as follows: [0003] 1) In foreign countries, the catalyst Pd / La0.5Pb0.5MnO3 with perovskite-type composite metal oxide as the carrier was mainly prepared by precipitation method, which catalyzed the oxidation of methyl glucoside to synthesize sodium formosylate, and further hydrolyzed the reaction to generate glucose aldehyde acid. Utilize this technique shortcoming mainly to be that the yield of glucuronic acid is too low, and purity is not high. [0004] 2) In China, the preparation technology of glucuronic acid mucopolysaccharide mainly utilizes natural raw materials, that is, extracts from animal tissues, and the main r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04
Inventor 张振武张海滨朱连明蒋贵信
Owner CHINA EASTAR HLDG GRP