New method for inhibiting synthesis of liver fat and preventing and treating fatty liver
A technology for fatty liver and its application, applied in the field of gene technology, can solve the problems such as the unclear pathogenesis of fatty liver
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Embodiment 1ZB
[0089] Example 1 Changes in Plasma Lipid Metabolism Indexes of ZBTB20 Hepatocyte-specific Knockout Mice
[0090] The analysis results of biochemical indicators such as plasma triglyceride, cholesterol and free fatty acid in ZBTB20 liver cell-specific knockout mice were measured, and compared with those of the control (wild type mice). Male mice and female mice were separately measured. And carry out statistical analysis.
[0091] The result is as figure 1 As shown, compared with the control group, the plasma triglyceride, cholesterol and free fatty acid of the ZBTB20 hepatocyte-specific knockout mice were all decreased. (P<0.05).
Embodiment 2
[0092] Example 2 Changes of Fat Content in the Liver Tissue of ZBTB20 Hepatocyte-specific Knockout Mice
[0093] The triglycerides in the liver tissue of ZBTB20 hepatocyte-specific knockout mice were extracted by biochemical methods, and the fat content in the liver tissue was detected under the condition of normal diet and high-fat diet respectively, and stained with oil red, from the morphology A more direct look at lipid content within liver tissue.
[0094] The result is as figure 2 A, 2B, 2C. The results showed that under normal diet and high-fat diet, the triglycerides in the liver tissue of ZBTB20 hepatocyte-specific knockout mice were all decreased, which was statistically significant (p<0.05). Using oil red staining, the results confirmed that the liver tissue of ZBTB20 hepatocyte-specific knockout mice had reduced lipids.
Embodiment 3ZB
[0095] Example 3 Analysis of fat synthesis rate in ZBTB20 hepatocyte-specific knockout mice
[0096] use 3 The H isotope tracer labeling method was used to detect the liver fatty acid synthesis rate and cholesterol synthesis rate of ZBTB20 hepatocyte-specific knockout mice.
[0097] Experimental results such as image 3 A, 3B. The results showed that the statistical analysis showed that compared with the control mice, the ZBTB20 hepatocyte-specific knockout mice had a decreased liver tissue fatty acid synthesis rate, which was statistically significant (P<0.05), and the cholesterol synthesis rate was significantly decreased, and had Statistically significant (P<0.01). This shows that ZBTB20 regulates the process of fatty acid de novo synthesis.
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