New method for inhibiting synthesis of liver fat and preventing and treating fatty liver

A technology for fatty liver and its application, applied in the field of gene technology, can solve the problems such as the unclear pathogenesis of fatty liver

Active Publication Date: 2011-01-12
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nevertheless, the pathogenesis of fatty liver in this field has not been clarified so far, and there are many gaps

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New method for inhibiting synthesis of liver fat and preventing and treating fatty liver
  • New method for inhibiting synthesis of liver fat and preventing and treating fatty liver
  • New method for inhibiting synthesis of liver fat and preventing and treating fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1ZB

[0089] Example 1 Changes in Plasma Lipid Metabolism Indexes of ZBTB20 Hepatocyte-specific Knockout Mice

[0090] The analysis results of biochemical indicators such as plasma triglyceride, cholesterol and free fatty acid in ZBTB20 liver cell-specific knockout mice were measured, and compared with those of the control (wild type mice). Male mice and female mice were separately measured. And carry out statistical analysis.

[0091] The result is as figure 1 As shown, compared with the control group, the plasma triglyceride, cholesterol and free fatty acid of the ZBTB20 hepatocyte-specific knockout mice were all decreased. (P<0.05).

Embodiment 2

[0092] Example 2 Changes of Fat Content in the Liver Tissue of ZBTB20 Hepatocyte-specific Knockout Mice

[0093] The triglycerides in the liver tissue of ZBTB20 hepatocyte-specific knockout mice were extracted by biochemical methods, and the fat content in the liver tissue was detected under the condition of normal diet and high-fat diet respectively, and stained with oil red, from the morphology A more direct look at lipid content within liver tissue.

[0094] The result is as figure 2 A, 2B, 2C. The results showed that under normal diet and high-fat diet, the triglycerides in the liver tissue of ZBTB20 hepatocyte-specific knockout mice were all decreased, which was statistically significant (p<0.05). Using oil red staining, the results confirmed that the liver tissue of ZBTB20 hepatocyte-specific knockout mice had reduced lipids.

Embodiment 3ZB

[0095] Example 3 Analysis of fat synthesis rate in ZBTB20 hepatocyte-specific knockout mice

[0096] use 3 The H isotope tracer labeling method was used to detect the liver fatty acid synthesis rate and cholesterol synthesis rate of ZBTB20 hepatocyte-specific knockout mice.

[0097] Experimental results such as image 3 A, 3B. The results showed that the statistical analysis showed that compared with the control mice, the ZBTB20 hepatocyte-specific knockout mice had a decreased liver tissue fatty acid synthesis rate, which was statistically significant (P<0.05), and the cholesterol synthesis rate was significantly decreased, and had Statistically significant (P<0.01). This shows that ZBTB20 regulates the process of fatty acid de novo synthesis.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses the use of zinc finger protein ZBTB20 for preparing a compound capable of inhibiting the synthesis of fat in the liver of mammal. The invention further discloses a method for filtering an efficient substance that inhibits the synthesis of fat in the liver of mammal based by taking the zinc finger protein ZBTB20 as a target point. The new method for controlling synthesis of liver fat and preventing and treating fatty liver firstly reveals the important functions of the zinc finger protein ZBTB20 in regulating the synthesis of liver cell fat, and thus providing an efficient target point for the research on medicaments preventing fatty liver.

Description

technical field [0001] The invention belongs to the field of gene technology, and more specifically, the invention relates to the function and application of a zinc finger protein. Background technique [0002] Fatty liver refers to the lesion of excessive accumulation of fat in liver cells due to various reasons. When lipids accumulate in the liver exceeding 5% of the wet weight of the liver or when more than 1 / 3 of the liver cells undergo fatty change in histology, Can be called fatty liver. Alcoholism, obesity, diabetes and hyperlipidemia are common causes of fatty liver. Fatty liver can be divided into alcoholic fatty liver and non-alcoholic fatty liver according to the etiology. The course of non-alcoholic fatty liver includes simple fatty liver, steatohepatitis, fatty liver fibrosis and liver cirrhosis. Its etiology is relatively complicated, and the disorder of lipid metabolism balance is the most basic and critical link in the occurrence and development of fatty li...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K38/16G01N33/68C12N5/071C12Q1/68A61K49/00A61P1/16
Inventor 章卫平周露婷张晔王燕卢银忠张海谢志芳
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap