Pichia farinose strain FL7 capable of dissolving inorganic phosphorus compounds and application thereof

A powdery Pichia pastoris and compound technology, applied in the field of microorganisms, can solve the problems of affecting phosphorus dissolving ability, loss, and failure to show the effect of increasing production, etc.

Inactive Publication Date: 2012-01-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many microorganisms have shown good ability to dissolve phosphorus compounds under laboratory conditions, but are less feasible for large-scale application in agricultural production, or cannot show significant yield increase effects
The reason may be that the simple screening conditions in the laboratory make the screened microorganisms unable to adapt to the complex soil environment; or the microorganisms themselves have poor adaptability and tolerance to different soil environments, which affects their ability to dissolve phosphorus At the same time, the phosphorus-dissolving ability of some bacteria is genetically unstable and may be lost with multiple passages

Method used

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  • Pichia farinose strain FL7 capable of dissolving inorganic phosphorus compounds and application thereof
  • Pichia farinose strain FL7 capable of dissolving inorganic phosphorus compounds and application thereof
  • Pichia farinose strain FL7 capable of dissolving inorganic phosphorus compounds and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, can dissolve the separation and screening of inorganic phosphorus compound bacterial strain

[0026] (1) Weigh 10g of agricultural waste compost samples (collect the agricultural waste compost samples from the experimental base of Tianjin Forestry and Fruit Research Institute), put them into a 250mL triangular flask (with glass beads) filled with 90mL sterile water, and let it stand After 20 minutes, place it in a 200rpm shaking incubator and shake it fully for 30 minutes to fully disperse the cells and make a bacterial suspension;

[0027] (2) Use a 5mL pipette to draw 5mL of the supernatant of the bacterial suspension obtained in the above step (1) into 45mL sterile water, mix well and carry out 10-fold decreasing dilution; take 10 -4 、10 -5 、10 -6 Each 100 μL of three dilutions of bacterial suspension was spread on Pikovskaya medium (glucose 10 g, yeast powder 0.5 g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O ...

Embodiment 2

[0032] Embodiment 2, bacterial strain FL7 utilizes the ability of cellulose

[0033] Strain FL7 was treated with 1% glucose, 1% soluble starch, 1% sucrose, 1% sodium carboxymethylcellulose, 1% cellulose, 1% sodium carboxymethylcellulose+0.01% glucose, 1% cellulose+0.01 % glucose as carbon source, all can grow, but the growth status is different. As shown in Table 1, the strain FL7 can rapidly grow and reproduce by utilizing glucose, but has poor utilization effects on soluble starch and sucrose. The strain FL7 also has a certain decomposing effect on sodium carboxymethylcellulose and cellulose, but the growth and reproduction of the strain FL7 is slow when it is used as the sole carbon source. it is good.

[0034] Table 1 The growth of strain FL7 using various carbon sources

[0035]

[0036] Note: The "+" sign in the table indicates the growth of the colony, and the more + signs, the larger the colony and the faster growth and reproduction.

Embodiment 3

[0037]Embodiment 3, the tolerance of bacterial strain FL7 to temperature, pH value, NaCl concentration, ammonium ion concentration

[0038] (1) Inoculate powdery Pichia farinosa strain FL7 on YEPD solid slant medium, culture at 30°C for 48 hours, so that the bacterial cells are in the logarithmic growth phase, and then transfer them by streaking on the plate Put it on the YEPD plate, culture it at different temperatures (28°C, 30°C, 35°C, 40°C, 45°C, 48°C, 50°C) for 7 days, observe the growth of the strain (see Table 2), and determine its growth potential. The highest temperature, indicating that the strain can still grow at 45°C.

[0039] By comparing the size of the cell density of the fermentation broth at different temperatures, it can be known ( image 3 ), the high temperature environment will inhibit the growth of the strain FL7, and the growth of the strain FL7 is better at a temperature of about 37°C.

[0040] (2) Then, the YEPD solid medium was adjusted to pH=5, pH...

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Abstract

The invention discloses a pichia farinose strain FL7CGMCC N0.4008 which can dissolve inorganic phosphorus compounds and application thereof. The experimental results show that the pichia farinose strain FL7CGMCC N0.4008 has the functions of dissolving inorganic phosphorus compounds and using celluloses, and has good tolerance to high temperature, high NaCL concentration, high ammonium ion concentration and alkaline environment.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a powdery Pichia yeast strain capable of dissolving insoluble inorganic phosphorus compounds and its application. Background technique [0002] Phosphate fertilizers will quickly react with inorganic acids and other substances after being applied to farmland to form insoluble phosphorus, which is difficult for plants to absorb. The application research on the application of microorganisms to improve the available phosphorus level of crop growth was first carried out in the former Soviet Union. In 1935, former Soviet researcher Mankina isolated a strain of Bacillus megatherium var.phosphaticum from soil, which can decompose nucleic acid and lecithin, and can increase the content of soluble phosphorus in soil after inoculation . Research on phosphate-solubilizing bacteria in my country began in the 1950s. Before 1950, the Northeast Institute of Agricultural Sciences isolat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C09K17/00C12R1/84C09K101/00
Inventor 乔建军孙丽范符玲刘富平张彦飞
Owner TIANJIN UNIV
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