Preparation method and application of oyster shell extract

Abstract

The invention relates to a preparation method of an oyster shell extract. The method comprises the following steps of: adding water-containing solution of alcohol into oyster shell powder in a material to liquid ratio of 1g:2-20mL, performing reflux extraction and filtering or centrifugally separating liquid out; and concentrating the obtained liquid under reduced pressure, extracting with an organic solvent, separating an organic solvent layer out, concentrating under reduced pressure and drying to obtain the oyster shell extract. Tests by a lissamine rhodamine B method indicate that the prepared oyster shell extract has the function of inhibiting cell proliferation of a plurality of tumor cell strains; and tests by a methyl thiazolyl tetrazolium method indicate that the oyster shell extract has the function of inhibiting cell proliferation of human umbilical vein endothelial cells. Experiments indicate that the oyster shell extract can play a role in resisting tumors by inhibiting tumor cell proliferation and angiogenesis. Therefore, the oyster shell extract of the invention can be taken as a cell proliferation inhibitor, an angiogenesis inhibitor and an anti-tumor agent.

Description

technical field

[0001] The invention relates to a preparation method of oyster shell extract and its application in the preparation of cell proliferation inhibitors, angiogenesis inhibitors and antitumor drugs. Background technique

[0002] Among mariculture species, shellfish account for more than 80% of mariculture due to its low input cost and high yield per unit area, and it has become an important channel for coastal fishermen to increase their income. Shellfish farming is dominated by oyster farming, but a large number of oyster shells left in the oyster production and processing process are not effectively utilized and properly disposed of except for some crushed oyster shells that are sold as animal feed or calcined lime, and are often dumped directly by processors. On both sides of the road, in the vacant land or in the abandoned ditches, the environment is seriously polluted, which directly affects the improvement of urban and rural living environment and the const...