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Method for processing sample of natural beta-carotene or lycopene fermentation liquor produced by fermenting blakeslea trispora

A bollidium and carotene technology, applied in chemical instruments and methods, hydrocarbon purification/separation, organic chemistry, etc., can solve problems such as high solvent toxicity and incomplete extraction, and achieve low solvent toxicity, complete soaking, and extraction. Efficient effect

Active Publication Date: 2013-07-03
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the β-carotene and lycopene produced by it are intracellular products, it is usually necessary to grind and extract the bacteria or soak in the homogenate with a high-speed homogenizer before the sample detection. The solvent used is highly toxic and easy to cause extraction. incomplete

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Quantitatively draw 1ml of β-carotene fermentation broth fermented by B. trispora into a 15ml polyplastic test tube, and draw three parts; quantitatively draw 1ml of lycopene fermentation broth fermented by B. three servings. Place the test tubes in a -10°C refrigerator for 1 hour, take them out to melt naturally, add 10ml of water for ultrasonic treatment for 5 minutes, centrifuge at 3000rpm for 10 minutes, discard the supernatant, add 5ml of absolute ethanol, centrifuge at 3000rpm for 10 minutes, discard the supernatant, and add Soak 5ml of ethyl acetate at 45°C for 50 minutes, filter and dilute the β-carotene sample at constant volume, and then detect it by spectrophotometry at a wavelength of 455nm. The lycopene samples were filtered by constant volume and detected by HPLC. The fermentation levels of lycopene were 1148, 1132, 1086 respectively, with an average of 1122 μg / ml.

Embodiment 2

[0026] Quantitatively draw 1ml of β-carotene fermentation broth fermented by B. trispora into a 15ml polyplastic test tube, and draw three parts; quantitatively draw 1ml of lycopene fermentation broth fermented by B. three servings. Place the test tubes in a -10°C refrigerator for 1 hour, take them out to melt naturally, add 10ml of water to sonicate for 25 minutes, centrifuge at 5000rpm for 10 minutes, discard the supernatant, add 10ml of absolute ethanol, centrifuge at 5000rpm for 10 minutes, discard the supernatant, and add Soak 10ml of ethyl acetate at 45°C for 40 minutes, filter and dilute the β-carotene sample at a constant volume, and then detect it by spectrophotometry at a wavelength of 455nm. The lycopene samples were filtered by constant volume and detected by HPLC. The fermentation levels of lycopene were 1157, 1179, 1084, respectively, with an average of 1140 μg / ml.

Embodiment 3

[0028] Quantitatively draw 1ml of β-carotene fermentation broth fermented by B. trispora into a 15ml polyplastic test tube, and draw three parts; quantitatively draw 1ml of lycopene fermentation broth fermented by B. three servings. Place the test tubes in a -18°C refrigerator for 1 hour, take them out to melt naturally, add 10ml of water to sonicate for 5 minutes, centrifuge at 5000rpm for 10 minutes, discard the supernatant, add 5ml of absolute ethanol, centrifuge at 5000rpm for 10 minutes, discard the supernatant, and add Soak 10ml of ethyl acetate at 65°C for 25 minutes, filter and dilute the β-carotene sample at a constant volume, and then detect it by spectrophotometry at a wavelength of 455nm. The lycopene samples were filtered by constant volume and detected by HPLC. The fermentation levels of lycopene were 1159, 1156, 1213 respectively, with an average of 1176 μg / ml.

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Abstract

The invention provides a method for processing a sample of natural beta-carotene or lycopene fermentation liquor produced by fermenting blakeslea trispora, which comprises the following steps of: absorbing fermentation liquor quantitatively, filling the fermentation liquor into a poly-plastic test tube, and freezing the fermentation liquor at below -10 DEG C; unfreezing the fermentation liquor naturally, adding water quantitatively, performing ultrasonic processing and centrifuging, and removing supernate; adding absolute ethanol into the centrifuged fermentation liquor for centrifuging, and removing supernate; and adding ethyl acetate for immersing at the temperature of between 45 and 80 DEG C, filtering in constant volume, and transferring the sample for detecting. The method has the advantages of short process flow, easy control of the process, complete sample immersing, high extraction efficiency and small toxicity of used solvent, and is suitable for processing the sample of the natural beta-carotene or lycopene fermentation liquor produced by fermenting the blakeslea trispora.

Description

technical field [0001] The invention relates to a sample processing method of fermented liquid, in particular to a sample processing method of natural beta-carotene or lycopene fermented liquid produced by B. trispora. Background technique [0002] Carotenoids (including β-carotene, lycopene, lutein, etc.) have many important physiological functions such as enhancing immunity and anti-oxidation. Among them, β-carotene is a fat-soluble carotenoid that exists widely and is closely related to human health. It not only has high medicinal value such as anti-cancer, anti-oxidation, and anti-radiation, but also can be Converted into vitamin A, it is an important source of vitamin A necessary for the human body. Therefore, β-carotene has been added as a food additive in various foods to play the role of pigment and nutritional fortifier. Type A nutritional pigments. In addition, lycopene is an isomer of carotene, because it does not have the β-carotene ring structure in β-caroten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24C07C11/21C07C7/00
Inventor 吴亚铭陈迎迎潘晨晓
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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