Preparation method of live vaccine for gosling plague and live vaccine prepared therefrom

A technology for live goose plague and gosling plague, applied in the field of bioengineering, can solve the problems of irregular, unstable and long time of duck embryo death, and achieve good immune protection effect, prevention of virulent infection, and good stability. Effect
CN101972473AInactive Publication Date: 2011-02-16SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SINOPHARM YANGZHOU VAC BIOLOGICAL ENG CO LTD
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a live vaccine for gosling plague and a live vaccine prepared therefrom. The preparation method of the live vaccine for the gosling plague comprises the steps of screening the breeding virus SYG61 of the gosling plague; diluting the breeding virus of the gosling plague viruses by utilizing sterilizing PBS (Phosphate Buffer Solution) in the ratio of 1 to 100; inoculating a well-developed goose embryo with 12 days, wherein each embryo flocking urine cavity is 0.2ml; the goose embryo dying in 48 to 144 hours is placed into a refrigerator with 2 to 8 DEG C for 4 to 24 hours; soaking the cooled goose embryo into a 5% bromo-geramine disinfectant for 5 to 10 minutes; placing into a sterile chamber after wiping drily; disinfecting a gas chamber eggshell part by utilizing 5% iodine tincture; opening the eggshell in a sterile procedure; eliminating an egg membrane and tearing a flocking urine membrane; sucking allantoic fluid and amniotic fluid; adding 5% sucrose skimmed milk as a stabilizing agent; simultaneously adding 1000 units / ml of penicillin and streptomycin respectively; fully agitating; quantitatively subpackaging; and rapidly refrigerating and drying in a vacuum state after subpackaging. The preparation method of the live vaccine for the gosling plague of the invention has the advantages of fewer steps and easy operation. Moreover, the prepared live vaccine for the gosling plague has the advantages of high yield, good stability, low cost and good immunity protection effect.
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Description

Technical field

[0001] The invention is a biological engineering technology field, which involves the preparation method of small goose plague vaccines and the live vaccine they prepared. Background technique

[0002] Goose plague is the acute or subacute semi -infectious diseases of the glutinous roses within 20 days caused by the small goose plague virus. Its incidence and mortality can be as high as 90 ~ 100 %.EssenceIn 1956, Professor Fang Yiyi first discovered the disease in Yangzhou. In 1961, Fang Yi, Wang Yongkun, etc. discovered the disease again in Yangzhou, and was separated from the virus. It was named the small goose plague.This disease has an outbreak in various provinces and world -raising countries across the country.

[0003] According to the International Veterinary Bureau (OIE) International Committee, mammals, poultry and bee A, and B diseases edited by the OIE Standard Committee "Diagnostic Test and Vaccine Standard Manual" (third edition, 1996)The content and...

Claims

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