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Test strip for measuring protein content of food and testing method thereof

A protein content and protein technology, applied in the field of food safety testing, can solve problems affecting the health of operators, cumbersome operation procedures, time-consuming and labor-intensive problems, and achieve the effects of improving detection work efficiency, simple sample processing, and shortening the detection cycle

Inactive Publication Date: 2011-02-16
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the results measured by the Kjeldahl method are not entirely protein, but also include some non-protein nitrogenous substances, such as urea nitrogen, free ammonia nitrogen, inorganic ammonia salt, etc. In order to increase the protein content of milk powder by adding a large amount of protein essence-melamine to illegally make huge profits, this led to the outbreak of the "Sanlu Milk Powder" incident that shocked the whole country and caused irreparable losses to the country and the people.
It can be seen that the traditional method is difficult to solve the problem of adulteration detection of protein in milk. At the same time, it also has disadvantages such as cumbersome operation process, time-consuming and laborious, and a large amount of harmful gas will be generated during the operation process, which will pollute the environment and affect the health of operators. Assay needs

Method used

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  • Test strip for measuring protein content of food and testing method thereof
  • Test strip for measuring protein content of food and testing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Rapid detection of protein in milk powder

[0018] 1. Accurately weigh 100mg milk powder sample, dissolve in 1000μL pH7.8 phosphate buffer, and mix well;

[0019] 2. Take a self-made protein detection test strip and place it flat on the detection platform;

[0020] 3. Use a pipette to accurately pipette 20 μL of the sample solution and drop it in the center of the filter belt of the test strip, and react at room temperature for 30 seconds;

[0021] 4. Use the portable color measuring instrument MICG1A to measure the reflectance of the test strip reaction zone after 30 seconds;

[0022] 5. According to the proportional relationship between the reflectance and the protein concentration, determine the protein content in the milk powder sample.

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Abstract

The invention provides a test strip for measuring protein content of a food and a testing method thereof. The test strip comprises a filter strip, a reaction strip, a water-absorbing strip and a substrate, wherein an indicator is adsorbed in the reaction strip and can perform specific reaction with protein in a sample; and the protein content is indicated by color changing. By using the test strip, the protein is tested by a portable color measurement instrument, the testing time is short and is generally less than 30 seconds, the result is accurate and rapid field testing of the protein in the food can be realized.

Description

technical field [0001] The invention relates to a detection technology for rapidly measuring protein content in food, belonging to the field of food safety detection. Background technique [0002] Food is the most basic necessity of people's life, and protein in food is the only source of nitrogen in the human body. Therefore, the amount of protein in food not only indicates the quality of the food, but also relates to the health of the human body. The National Food Hygiene Standard also stipulates the content of protein in some foods. Among them, the determination of protein in animal milk and animal milk products is a must-test item for the analysis of food nutritional components. At present, the milk powder quality judgment methods used by daily consumers and quality inspection departments on the spot mainly rely on sensory observation (hand feel, color, smell, taste, etc.), and then the quality inspection department randomly checks and samples and sends them to the tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N33/68G01N21/47
Inventor 何保山谢岩黎李雪琴韩小贤李漫男
Owner HENAN UNIVERSITY OF TECHNOLOGY
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