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Method for synthesizing fish oil ethyl ester by lipase catalysis

The technology of a kind of fish oil ethyl ester and synthesis method is applied in the field of lipase-catalyzed synthesis of fish oil ethyl ester, which can solve the problems of large amount of raw materials, serious environmental pollution, high production cost, etc., achieve less usage, eliminate environmental pollution, and reduce production cost Reduced effect

Active Publication Date: 2011-02-23
BEIJING CTA NEW CENTURY BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above-mentioned research in my country for the preparation of high-content ethyl polyenoate mainly uses chemical catalysts to carry out transesterification reactions, which have the disadvantages of large raw material consumption, high production costs, and serious environmental pollution. Therefore, it is necessary to study a green and pollution-free The synthetic method of fish oil ethyl ester

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the synthesis of fish oil ethyl ester

[0026] (1) Synthesis of fish oil ethyl ester

[0027] Accurately weigh 500 g of crude fish oil (18.23% EPA and 7.89% DHA containing mass percent), 250 g of dehydrated alcohol (the mass and number ratio of crude fish oil and dehydrated alcohol is 2: 1) and Yarrow lipolytica fat Enzyme (enzyme activity is 100000U / g, Beijing Kaitai New Century Biotechnology Co., Ltd., LS-10) 0.5g (the addition of Yarrow lipolytica lipase is 0.1% of the quality of crude fish oil) is placed in the reaction vessel , Carry out transesterification reaction at 25°C and 100rpm. After reacting for 40 h, a mixed solution containing fish oil ethyl ester was obtained. The content of EPA ethyl ester and DHA ethyl ester was detected by HPLC, the content of EPA ethyl ester was 17.51%, and the content of DHA ethyl ester was 7.15%.

[0028] (2) Separation and purification of fish oil ethyl ester

[0029] Place 250 g of the fish oil ethyl ester mixt...

Embodiment 2

[0030] Embodiment 2, the synthesis of fish oil ethyl ester

[0031] (1) Synthesis of fish oil ethyl ester

[0032] Accurately take by weighing crude fish oil (containing the EPA of 19.38% and the DHA of 6.29% by mass percentage) 100kg, 95% ethanol 17kg (the mass and number ratio of crude fish oil and 95% ethanol is 6: 1) and Yarrow lipolytica fat Enzyme (enzyme activity is 200000U / g, Beijing Kaitai New Century Biotechnology Co., Ltd., LS-20) 3kg (the addition of Yarrow lipolytica lipase is 3% of the quality of crude fish oil) is placed in reaction vessel, The transesterification reaction was carried out at 40° C. and 30 rpm. After 10 h of reaction, a mixed solution containing fish oil ethyl ester was obtained. The content of EPA ethyl ester and DHA ethyl ester was detected by HPLC, the content of EPA ethyl ester was 18.76%, and the content of DHA ethyl ester was 5.52%.

[0033] (2) Separation and purification of fish oil ethyl ester

[0034] Place 50 kg of the fish oil eth...

Embodiment 3

[0035] Embodiment 3, the synthesis of fish oil ethyl ester

[0036] (1) Synthesis of fish oil ethyl ester

[0037] Accurately take by weighing crude fish oil (containing the EPA of 5.43% and the DHA of 21.69% by mass percentage) 500kg, dehydrated alcohol 83Kg (the mass and number ratio of crude fish oil and dehydrated alcohol is 6: 1) and Yarrow lipolytica fat Enzyme (enzyme activity is 170000U / g, Beijing Kaitai New Century Biotechnology Co., Ltd., LS-17) 5kg (the addition of Yarrow lipolytica lipase is 1% of the quality of crude fish oil) is placed in the reaction vessel, Then add 50Kg of water with 10% crude fish oil quality, and carry out transesterification reaction at 65° C. and 230 rpm. After reacting for 30 h, a mixed solution containing fish oil ethyl ester was obtained. The content of EPA ethyl ester and DHA ethyl ester was detected by HPLC, the content of EPA ethyl ester was 4.88%, and the content of DHA ethyl ester was 19.53%.

[0038](2) Separation and purificat...

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Abstract

The invention discloses a method for synthesizing fish oil ethyl ester. The method comprises the following steps of: performing an ester exchange reaction on crude fish oil and ethanol under the catalysis of lipase to obtain the fish oil ethyl ester; and freezing and crystallizing the obtained fish oil ethyl ester. The method for synthesizing the fish oil ethyl ester by lipase catalysis is different from the conventional chemical method for synthesizing the fish oil ethyl ester, greatly reduces the using amount of raw materials and production cost, improves the quality of products, and basically eliminates environmental pollution.

Description

technical field [0001] The invention relates to a synthesis method of fish oil ethyl ester, in particular to a lipase-catalyzed synthesis method of fish oil ethyl ester. Background technique [0002] Deep-sea fish oil is rich in omega-3 series unsaturated fatty acids (PUFA) such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and its important functions in human biology have been recognized by the world . EPA has the functions of preventing coronary heart disease, lowering blood pressure, eliminating fatigue, preventing arteriosclerosis and cerebral thrombosis, and anti-cancer, while DHA can effectively promote the intellectual development of infants, improve brain function, and improve memory. In a word, EPA and DHA have good health effects in preventing cardiovascular disease and improving intelligence of infants. [0003] The content of EPA and DHA in natural fish oil is relatively low (20%-30%), and its medical and health effects are not ideal. In order...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 闵瑞周浩朱琳张增伟张文波顾燕松
Owner BEIJING CTA NEW CENTURY BIOTECH