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Mactra quadrangularis protein polypeptide with antioxidant activity and preparation method and application thereof

An anti-oxidant activity, the technology of four-cornered clam, applied in the field of four-cornered clam, can solve the problems of cell membrane integrity damage, change structure and permeability, attack DNA molecules, etc.

Inactive Publication Date: 2012-06-06
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, the development and application of four-cornered clams mostly use software as raw materials to process canned food, condiments, feed additives, etc., while the research and development of medicine and health products for four-cornered clams with the goal of medicinal functions are less
The research on the active substances of the four-cornered clam mostly focuses on the small molecule chemical components (such as taurine and EPA), and there are few reports on the research on its biologically active macromolecules, such as proteins and polysaccharides
However, too many active oxygen free radicals will have destructive behavior. Free radicals with unpaired electrons can trigger the peroxidation reaction of unsaturated fatty acid lipids on the cell membrane and combine with enzymes on the membrane, resulting in the destruction of the integrity of the cell membrane. Change the structure and permeability; in addition, free radicals can also react with the protein in the cell to cause protein denaturation, thereby making the normal function of the cell impossible, and free radicals can also attack DNA molecules, causing gene mutations

Method used

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  • Mactra quadrangularis protein polypeptide with antioxidant activity and preparation method and application thereof
  • Mactra quadrangularis protein polypeptide with antioxidant activity and preparation method and application thereof
  • Mactra quadrangularis protein polypeptide with antioxidant activity and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 A clam protein polypeptide with antioxidant activity, which is prepared by the following method:

[0075] The specific steps are as follows: wash the clam soft body, stir and homogenate to obtain a homogenate, then add trypsin accounting for 0.25% by weight of the homogenate of clam, enzymolyze at pH 8.5 and temperature 45°C for 80 minutes, and the enzymolysis ends Then inactivate the enzyme in a water bath at 90°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, take the supernatant and perform ultrafiltration with a molecular weight cut-off of 10-30kDa. The ultrafiltrate is concentrated under reduced pressure and spray-dried to obtain the clam protein polypeptide.

Embodiment 2

[0076] Embodiment 2 A clam protein polypeptide with antioxidant activity, which is prepared by the following method:

[0077] The specific steps are: wash the clam soft body, stir and homogenate to obtain a homogenate, then add trypsin accounting for 0.5% of the weight of the homogenate of the clam, enzymolyze at pH 8.0 and temperature 55°C for 90 minutes, and the enzymolysis ends After that, inactivate the enzyme in a water bath at 100°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, take the supernatant and perform ultrafiltration with a molecular weight cut-off of 10-50kDa, concentrate the ultrafiltrate under reduced pressure, and spray-dry to obtain the clam protein polypeptide.

Embodiment 3

[0078] Embodiment 3 A clam protein polypeptide with antioxidant activity, which is prepared by the following method:

[0079] The specific steps are as follows: wash the clam soft body, crush and homogenate to obtain a homogenate, then add trypsin accounting for 0.1% of the weight of the homogenate of clam, enzymolyze at pH 7.5 and temperature 55°C for 70 minutes, and the enzymolysis ends Then inactivate the enzyme in a water bath at 90°C for 10 minutes, centrifuge at 4000rpm for 15 minutes, take the supernatant and perform ultrafiltration with a molecular weight cut-off of 30-50kDa, concentrate the ultrafiltrate under reduced pressure, and spray dry to obtain the clam protein polypeptide.

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Abstract

The invention discloses mactra quadrangularis protein polypeptide with antioxidant activity and a preparation method and application thereof. The mactra quadrangularis protein polypeptide is prepared by the following steps of: washing mactra quadrangularis clam, grinding and homogenizing; adding trypsin in an amount which is 0.05 to 0.5 percent of the weight of the homogenized solution; performing enzymolysis at the pH of between 7.5 and 8.5 and at the temperature of between 45 and 55 DEG C for 30 to 90 minutes; deactivating the enzyme in water bath at the temperature of between 90 and 100 DEG C for 10 minutes; centrifuging; ultra-filtering the supernatant; intercepting the ultra-filtrate with molecular weight of between 10 and 50kDa; concentrating under reduced pressure; and performing spray-drying. Experiment results show that the mactra quadrangularis protein polypeptide provided by the invention has high antioxidant activity and small molecular weight and can be easily absorbed byhuman bodies after being taken; and through a great amount of experimental screening, the provided preparation method of the mactra quadrangularis protein polypeptide has rational technological parameter design and high operability, can fully utilize the rich resources of the mactra quadrangularis and has great economic effect and social effect.

Description

technical field [0001] The invention relates to clam square, in particular to a polypeptide with antioxidant activity obtained by hydrolyzing clam protein and its preparation method and application. Background technique [0002] Four-cornered clams, also known as square clams, white clams or four-cornered clams, are commonly known as white clams, mud clams, and cloth pigeon heads. The shell is slightly quadrangular, the two shells are equal, the sides expand, and the shell surface is smooth and white. It is a common marine shellfish mollusc and is widely distributed in the coastal waters of the north and south of my country. There are many tidal flats along the coast of Jiangsu, and the four-cornered clam is the dominant shellfish. Large-scale artificial breeding has been realized, with an output of up to 80,000 tons per year. The four-cornered clam is an important edible economic shellfish. Its soft meat is tender and delicious, rich in nutrition, and contains protein, fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P39/06A23L1/305A23L33/18
Inventor 王令充吴皓郑文文
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE