Radish seed extract as well as preparation method and application of radish seed extract

A technology of radish seeds and extracts, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve the problems of leukopenia, light sensitivity, etc., and achieve the effects of low cost, simple preparation method, and wide source of materials

Inactive Publication Date: 2011-03-02
HAINAN ZHUFENG GARDEN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Griseofulvin is the most widely used systemic antifungal drug, but associated side effects such as headache, gastrointestinal pain, photosensitivity, rash or leukopenia, angioedema

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, preparation and functional verification of radish seed extract

[0032] 1. The preparation procedure is as follows:

[0033] 1. Disinfection

[0034] Soak the radish seeds with sterile deionized water for 45 minutes to soften the sundries attached to the surface of the seeds, remove the water, rinse the soaked seeds twice with 75% ethanol aqueous solution, and then rinse the seeds twice with sterile deionized water to obtain Sterilized radish seeds.

[0035] 2. Mix the sterilized radish seeds and sterile deionized water according to the volume ratio of 1:5, and let stand for 12 hours. Beat all the substances in the soaking container together into a homogenate, centrifuge the obtained homogenate at a speed of 8000r / min for 5min, with a radius of rotation of 100mm, and take the supernatant to obtain the radish seed extract, which is sealed and stored.

[0036] 2. Antibacterial experiment

[0037] The radish extract prepared in Experiment 1 was directly u...

Embodiment 2

[0064] Embodiment 2, preparation and functional verification of radish seed extract

[0065] One, the preparation steps are as follows:

[0066] 1. Disinfection

[0067] Soak the radish seeds in sterile deionized water for 30 minutes to soften the impurities attached to the surface of the seeds, remove the water, rinse the soaked seeds twice with 75% ethanol aqueous solution, and then rinse the seeds twice with sterile deionized water to obtain Sterilized radish seeds.

[0068] 2. Mix the sterilized radish seeds with sterile deionized water at a volume ratio of 1:4, and let stand for 12 hours. Beat all the substances in the soaking container together into a homogenate, centrifuge the obtained homogenate at a speed of 5000r / min for 5min with a radius of rotation of 80mm, take the supernatant to obtain the radish seed extract, and keep it sealed.

[0069] 2. Antibacterial experiment

[0070] The method is consistent with that described in Example 1. There was no significant...

Embodiment 3

[0076] Embodiment 3, preparation and functional verification of radish seed extract

[0077] One, the preparation steps are as follows:

[0078] 1. Disinfection

[0079] Soak the radish seeds in sterile deionized water for 60 minutes to soften the impurities attached to the surface of the seeds, remove the water, rinse the soaked seeds twice with 75% ethanol aqueous solution, and then rinse the seeds twice with sterile deionized water to obtain Sterilized radish seeds.

[0080] 2. Mix the sterilized radish seeds and sterile deionized water according to the volume ratio of 1:6, and let stand for 12 hours. Beat all the substances in the soaking container together into a homogenate, centrifuge the obtained homogenate at a speed of 11,000 r / min for 5 minutes, with a radius of rotation of 150 mm, and take the supernatant to obtain the radish seed extract, which is sealed and stored.

[0081] 2. Antibacterial experiment

[0082] The method is consistent with that described in Ex...

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PUM

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Abstract

The invention relates to a radish seed extract as well as a preparation method and an application of a radish seed extract. The preparation method of the radish seed extract comprises the following steps: soaking disinfected radish seeds with water; pulping all the substances in a soaking container into homogenate; centrifuging the obtained homogenate; and taking supernate, thus obtaining the radish seed extract. Experiments prove that the extract has the function of restraining funguses capable of causing skin diseases; an inhibition zone experiment indicates that the inhibition zone diameter of the extract is obviously larger than that of a control group; a skin disease therapeutic experiment indicates that the therapeutic effect of the extract is obviously better than that of the control group, the curative effect is rapid, and plainness without side effect can be realized; and in addition, the preparation method of the extract is simple, the raw material source is wide, and the cost is low, thus the extract in the invention has the characteristic of wide application prospect in the field of treating fungal skin diseases.

Description

technical field [0001] The invention relates to a radish seed extract and its preparation method and application. Background technique [0002] Superficial fungi that parasitize the keratinous tissue of the skin are called dermatophytes. The skin ringworm caused by it, especially tinea manus, pedis (commonly known as athlete's foot), and jock itch are the most common fungal diseases in humans. The dermatophytes are divided into the genus Trichophyfon, Epidermophyfon and Microsporum (Microsporum) 3 genera. The main causes of skin fungal diseases in my country are Trichophyfon gypseum, Trichophyfon purpureatum, Epidermophyfon floccosum, Microsporum audouini, Microsporum canis (Microsporum canis) and gypsum-like Microsporum (Microsporum gypseum) and so on. [0003] Topical antifungal agents for most skin infections are chemical agents such as imidazoles (miconazole, clotrimazole, econazole, ketoconazole), ciclopirox, naftifine, or terbix Nafine, newer systemic agents includi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61P31/10A61P17/00A61K131/00
Inventor 杨逢春余文刚宋希强徐诗涛杨泽秀
Owner HAINAN ZHUFENG GARDEN TECH
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