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Application of nano reduced graphene oxide serving as antibacterial material

A technology of graphene oxide and antibacterial materials, applied in applications, animal repellents, plant growth regulators, etc., can solve problems such as toxicity and achieve good antibacterial properties

Inactive Publication Date: 2011-03-30
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the toxicity of these nano-antibacterial materials has also attracted the attention of researchers. Studies have shown that most nano-antibacterial materials have strong toxic effects on mammalian cells.

Method used

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  • Application of nano reduced graphene oxide serving as antibacterial material
  • Application of nano reduced graphene oxide serving as antibacterial material
  • Application of nano reduced graphene oxide serving as antibacterial material

Examples

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Effect test

preparation example Construction

[0029] According to another aspect of the present invention, a kind of preparation method of nano-reduced graphene oxide film specifically comprises the following steps:

[0030] (1) Suction filtration: Suction filtration of a certain volume of prepared nano-reduced graphene oxide suspension with a 0.22 μm PVDF membrane.

[0031] (2) drying: the above-mentioned PVDF film covered with nano-reduced graphene oxide was vacuum-dried overnight at room temperature;

[0032] (3) Film formation: the nano-reduced graphene oxide film is peeled off from the PVDF film with sharp tweezers to obtain the nano-reduced graphene oxide film of the present invention.

[0033] The antibacterial performance test of the nano-reduced graphene oxide film prepared according to the present invention adopts Gram-negative bacteria, Eschrrichia coli DH5α (E.coli DH5α), and the specific steps include:

[0034] (1) Escherichia coli is cultured in suspension. After overnight, the Escherichia coli is collected...

Embodiment 1

[0039] The preparation of embodiment 1 nano-reduced graphene oxide suspension

[0040]Weigh 4g of graphite powder and disperse in 24ml of concentrated H 2 SO 4 , then add 8g K 2 S 2 o 4 and 8g of P 2 o 5 , stirred in a water bath at 75°C for 4 hours; the above mixture was cooled to room temperature, and washed with triple distilled water (Milli-Q@Synthesis A10 TM , Millipore) was diluted to 300mL, and then filtered with a 0.22μm PVDF filter membrane (0.22μm, diameter 50mm, 100 pieces / box, Shanghai Jierui Bioengineering Co., Ltd.), and the filtered precipitate was vacuumed at 60°C (DZF-6050 type vacuum Oven, Shanghai Jinghong Experimental Equipment Co., Ltd.) to dry overnight to obtain the preoxidized product.

[0041] Weigh 12gKMnO 4 , dispersed in 92 mL of ice-concentrated H 2 SO 4 , then add 2g of the above preoxidation product, stir in ice bath for 15min, then add 2g NaNO 3 After stirring for 2 hours, add 200 mL of triple distilled water, and finally add 560 mL o...

Embodiment 2

[0045] Antibacterial test of embodiment 2 nano-reduced graphene oxide suspension

[0046] Suspension culture Escherichia coli (DH5αglycerobacteria, Beyuntian Biotechnology Research Institute), after overnight, centrifuge at 12000rpm for 1min to collect Escherichia coli, resuspend with sterilized saline (0.9% NaCl solution), and then dilute 10 times Finally, measure OD600 to determine the number of E. coli, and then adjust the concentration of bacteria to 10 8 / mL;

[0047] Take 100 μL of the above bacterial solution and disperse it in 850 μL of reduced graphene oxide suspension, then add 50 μL of 18% NaCl solution to maintain the mass fraction of NaCl in the mixed system at 0.9%, mix well and place on a constant temperature shaker at 37°C ( 200rpm) to incubate for 2h;

[0048] Take 100 μL of the above bacterial solution and incubate directly on a 37°C constant temperature shaker (THZ-C constant temperature oscillator, Jiangsu Taicang Experimental Equipment Factory) for 2h-3h...

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Abstract

The invention discloses the application of nano reduced graphene oxide serving as an antibacterial material, in particular relates to the application of nano reduced graphene oxide suspension or a nano reduced graphene oxide membrane serving as an antibacterial material. The preparation method of the nano reduced graphene oxide suspension comprises the following steps of: pre-oxidation of graphite, separation of a pre-oxidized product, re-oxidation, purification of an oxidation product and reduction of the oxidation product. The nano reduced graphene oxide film is prepared by performing suction filtration on the nano reduced graphene oxide suspension by using a polyvinylidene fluoride (PVDF) membrane of which the mesh is 0.22 mu m. Through the application of the nano reduced graphene oxide serving as the antibacterial material, the nano reduced graphene oxide serving as an additive can be prepared into a product with an antibacterial property or prepared into an antibacterial coating material, which is applied to medicines and daily life.

Description

technical field [0001] The invention relates to an antibacterial material, in particular to the application of nano-reduced graphene oxide as an antibacterial material. Background technique [0002] With the development of microbiology, people's research and understanding of microbes are constantly improving. While making full use of beneficial microbes, they are also very vigilant against pathogenic microbes. How to prevent pathogenic microbes from invading the human body has become an urgent problem to be solved. However, in the process of preventing and controlling pathogenic microorganisms, many problems have occurred due to the extensive use of antibiotics. One of the most prominent is the emergence of a large number of drug-resistant strains of pathogenic microorganisms; at the same time, the extensive use of antibiotics has also caused serious environmental pollution, which has caused harm to human health and quality of life. How to solve these problems, people are r...

Claims

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Application Information

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IPC IPC(8): A01N59/00A01P1/00
Inventor 黄庆樊春海胡文兵彭程
Owner SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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