Method for preparing fish protein powder rich in fishskin collagen

A technology of collagen and fish protein powder, applied in fish protein components, protein food components, food science, etc., can solve the problems of insufficient extraction and long production cycle, and achieve wide raw material adaptability, low production cost, The effect of high protein extraction rate

Inactive Publication Date: 2011-04-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the fish skin scraps produced in the actual process of fishery processing usually exist in the state of mixed raw materials, which contain fish skin, fish scales, fish fins, fish bones and a small amount of fish meat, therefore, for

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Tilapia skin 100g, add 1000ml saline solution (containing 1%NaCI and 1%Na 2 CO 3 ), soak evenly for 12 hours, drain with silk cloth, add 1000ml of deionized water to soak evenly, drain each time until the pH reaches neutral, drain, grind with a meat grinder, add 300ml of deionized water, stir and heat up to 55°C, add 0.225g of neutral protease, incubate in a 1000w microwave extractor for 0.5 hours, cool down to 45°C, add 0.025g of bromelain, continue microwave treatment for 0.5 hours, boil and denature for 0.5 hours, cool down to 70°C and add 8g of activated carbon powder was decolorized for 30 minutes, then 2g of diatomaceous earth was added, vacuum filtered, concentrated under reduced pressure, and freeze-dried to obtain protein powder.

Embodiment 2

[0010] Add 100g of cod skin and fish scales, add 1000ml saline solution (containing 1%NaCl and 1%Na 2 CO 3 ), soak evenly for 12 hours, drain with silk cloth, add 1000ml of deionized water to soak evenly, drain each time until the pH reaches neutral, drain, grind with a meat grinder, add 300ml of deionized water, stir and heat up to 55°C, add 0.125g of neutral protease, incubate in a 1000w microwave extractor for 0.5 hours, cool down to 45°C, add 0.125g of bromelain, continue microwave treatment for 0.5 hours, boil and denature for 0.5 hours, cool down to 70°C and add 8g of activated carbon powder was decolorized for 30 minutes, then 2g of diatomaceous earth was added, vacuum filtered, concentrated under reduced pressure, and freeze-dried to obtain protein powder.

Embodiment 3

[0012] Tilapia skin plus fish scales and fish fins are 100g altogether, add 1000ml saline solution (containing 1%NaCl and 1%Na 2 CO 3 ), soak evenly for 12 hours, drain with silk cloth, add 1000ml of deionized water to soak evenly, drain each time until the pH reaches neutral, drain, grind with a meat grinder, add 300ml of deionized water, stir and heat up to 55°C, add 0.2g of neutral protease, incubate in a 1000w microwave extractor for 0.5 hours, cool down to 45°C, add 0.05g of bromelain, continue microwave treatment for 0.5 hours, boil and denature for 0.5 hours, cool down to 70°C and add 8g of activated carbon powder was decolorized for 30 minutes, then 2g of diatomaceous earth was added, vacuum filtered, concentrated under reduced pressure, and freeze-dried to obtain protein powder.

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PUM

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Abstract

The invention discloses a method for preparing fish protein powder rich in fishskin collagen from a fishskin raw material which contains fish scale, fish fins, fish bone and a small amount of fish meat. The method comprises the following process steps: soaking in salt and alkaline solution, soaking in deionized water for desalination, stirring, modifying in microwaves, degrading complex enzyme at constant temperature, boiling, decolorizing, deodorizing, concentrating and drying. Concretely, the method comprises: transferring a fishskin raw material to salt and alkaline solution which contains 1 percent of NaCl and 1 percent of Na2CO3 and of which the weight is 10 times that of the fishskin raw material, soaking the fishsikn raw material for 12 hours, then soaking the fishskin raw material in deionized water, and washing the fishskin raw material till the pH value of the fishskin raw material is 7; draining, grinding in a meat grinder, adding deionized water in an amount which is 3 times that of the grind material, heating to the temperature of 55 DEG C, keeping the temperature constant, adding neutral protease in an amount which is 0.125 to 0.225 percent based on the weight of the fishskin raw material, and reacting for 0.5 hour under the action of microwaves at a power of 1,000 W; cooling to 45 DEG C, adding bromelain in an amount of 0.0258 to 0.125 percent based on the weight of the fishskin raw material, and continuing microwave treatment for 0.5 hour; and boiling and modifying for 0.5 hours, decolorizing by active carbon for 30 minutes, filtering with aid from kieselguhr, concentrating under reduced pressure, drying and obtaining fish protein powder. The protein extraction rate is more than 95 percent, the product is white powder, free from fish smell and rich in fishskin collagen, the average molecular weight of the product is less than 6,000Da, and the product can dissolve in water instantly.

Description

technical field [0001] The invention relates to a preparation method of fish protein powder rich in fish skin collagen, belonging to the field of biochemical industry. Background technique [0002] Collagen protein is an important functional protein, its extracts and degradation products have been widely used in industrial fields such as medicine, food, daily chemical industry, biosynthesis, such as edible protein, cosmetics and food additives and pet feed Additives etc. The raw materials of collagen products currently produced mainly come from the skin, bone and tendons of terrestrial mammals such as pigs and cattle. Due to the deterioration of the global ecological environment in recent years, many epidemics such as mad cow disease and foot-and-mouth disease have occurred, making the production of collagen from land The risk of extracting collagen from raw animal skin and bone increases. As a result, people began to seek to extract collagen from aquatic animals, which ha...

Claims

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Application Information

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IPC IPC(8): A23J1/04
Inventor 陈西广刘成圣程晓杰于乐军
Owner OCEAN UNIV OF CHINA
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