Method for detecting cations in aqueous solution
A detection method and positive ion technology, applied in the field of ion detection, can solve problems such as water quality detection, expansion of pollution sources, ion interference, etc., and achieve the effects of sensitive and rapid detection, high detection sensitivity, and low detection cost
Inactive Publication Date: 2011-04-20
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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Problems solved by technology
When the above methods are used to detect toxic cations in aqueous systems, the sample preparation steps are complicated, the samples have poor water solubility, serious ion interference, and usually require large and expensive equipment
These limitations are very unfavorable for the rapid detection of toxic cations in aqueous systems, because many units or individuals with insufficient conditions or immature technologies cannot detect water quality, resulting in the expansion of pollution sources; moreover, real-time and on-site water quality cannot be realized by using these methods. detection
Method used
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preparation example Construction
[0035] The preparation of the detection solution includes the following steps:
[0036] (1) Preparation of gold nanoparticles or silver nanoparticles;
[0037] (2) selecting a nucleic acid molecule comprising a specific sequence according to the detected cation;
[0038] (3) Using sulfhydryl groups to realize the coupling of specific sequence nucleic acid molecules to gold nanoparticles or silver nanoparticles;
[0039] (4) Preparation of detection solution.
[0040] In the above steps, the preparation method of gold nanoparticles or silver nanoparticles is well known to those skilled in the art, and will not be repeated here.
[0041] Nucleic acid molecules containing specific sequences in the present invention can be purchased from Link Technologies in the UK or TriLink BioTechnologies in the United States.
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The invention relates to a method for detecting cations in aqueous solution. On the basis of a nano-nucleic acid copolymer system, by using mutual complexation of nucleic acid and the cations, the melting temperature of the system rises, so that the color change of the solution is determined by naked eyes or simple instruments and equipment according to the lag characteristic of the color change of the solution and toxic cations in the aqueous solution system can be rapidly detected.
Description
technical field [0001] The invention relates to an ion detection method, in particular to a detection method for cations in aqueous solution. Background technique [0002] Water is closely related to the survival, health and development of human beings. Water environmental pollution and water resource shortage are two major problems facing the global fresh water resources. China's per capita water resources are only 1 / 4 of the world's average level. With the rapid development of my country's economy, the increase of population, the gradual improvement of people's living standards, the acceleration of industrialization and urbanization, the water consumption has increased sharply, and sewage Emissions have also increased accordingly, exacerbating the shortage of fresh water resources and the pollution of the water environment. Therefore, protecting and rationally utilizing limited water resources and reducing and controlling water pollution have become one of the urgent probl...
Claims
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Login to View More IPC IPC(8): C12Q1/68G01N21/29
Inventor 曾乐勇吴爱国
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI