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Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein

A technology of plant immune protein and protein, which is applied in the field of protein, to improve plant immunity, facilitate industrial promotion and application, and achieve the effect of low cost

Active Publication Date: 2011-04-27
北京东方先科科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] To sum up, the existing studies on NAC protein have shown that, in addition to its basic protein functions in the field of biological genetics, NAC protein can also induce abnormal growth of tumor cells and reduce cell death in humans, and it can also participate in plant growth. Response to adversity, but there have never been related reports documenting or showing that NAC protein has the function of inducing or activating plant immune response

Method used

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  • Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein
  • Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein
  • Application of nascent polypeptide-associated complex (NAC) protein as vegetable immune protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Application of NAC protein to improving immunity of tomato Botrytis cinerea

[0020] The NAC protein was diluted to 30ppm with water, and the tomato seedling leaves (5-6 leaves) were sprayed with the diluted protein solution, and water was used as a control, and 30 seedlings were used for each treatment. After processing 7d and 14d, spray the Botrytis cinerea spore suspension (10 5 Individual / ml) was inoculated on the tomato seedlings, kept moist for 48h, carried out disease index survey after one week, calculated control effect, each treatment was all repeated three times, and the experimental results after treatment are shown in Table 1 below.

[0021] Table 1: Experimental control results of NAC protein-induced disease resistance in tomato

[0022]

[0023] In Table 1 above:

[0024]

[0025] (For the grading method of diseased leaves, please refer to figure 1 )

[0026] where Pt 1 Represent the disease index after the method of embodiment 1 i...

Embodiment 2

[0028] Embodiment 2: The application of NAC protein to improving the immunity of pepper against Phytophthora

[0029] Dilute the NAC protein to 25ppm with clear water, spray the pepper (variety: Zhongjiao No. The disease index was investigated on the 7th day, and the control effect was calculated as shown in Table 2 below. The experimental data of the investigation were all analyzed by the DPS data processing system at the 5% level.

[0030] Table 2: Experimental control of the effect of NAC protein on the induction and resistance of pepper blight

[0031]

[0032] In Table 2 above:

[0033]

[0034] where Pt 2 Represent the disease index after the method of embodiment 2 is processed; Pt CK Indicates the disease index of the water control.

[0035] The grading criteria for Phytophthora capsicum disease at the seedling stage are (in whole plants):

[0036] Level 0: no disease;

[0037] Level 1: The rhizomes of the seedlings turn black slightly, and the leaves do ...

Embodiment 3

[0043] Embodiment 3: The application of NAC protein to improving tomato anti-tobacco mosaic virus (TMV) immunity

[0044] First, cultivate tomato seedlings with 3 to 4 leaves (about 6cm to 8cm in length) with sterilized soil, then dilute the NAC protein to 30ppm with water, and spray the tomato seedlings (3 to 4 leaves) with the diluted protein solution , treated with clean water as a control. Tobacco mosaic virus (TMV) was inoculated on the 7th day and 14th day after the spraying treatment, and each treatment was repeated three times. The disease index of each tomato seedling was investigated on the 7th day after virus inoculation, and the induced resistance effect was calculated as shown in Table 3 below.

[0045] Table 3: Experimental control results of NAC protein on tomato-induced antiviral activity

[0046]

[0047] Refer to Example 1 for the calculation methods of the disease index and the induction effect in Table 3 above.

[0048] It can be seen from the experim...

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Abstract

The invention relates to a specific application of a nascent polypeptide complex protein, in particular to an application of a nascent polypeptide-associated complex (NAC) protein as a vegetable immune protein. Specifically, the application can be an application of the NAC protein in improving the gray mold resisting immunity of tomatoes, an application of the NAC protein in improving the tobacco mosaic virus resisting immunity of tomatoes, or an application of the NAC protein in improving the phytophthora capsici resisting immunity of chilli. The application process has simplicity of operation, low cost and good effect.

Description

technical field [0001] The present invention relates to the use of a protein, in particular to the specific use of a nascent polypeptide complex protein. Background technique [0002] Nascent polypeptide-associated complex was first purified from bovine brain by affinity purification, and it was also the first report that the protein could compete with signal recognition particle SRP (signal recognition particle) for binding to ribosomes synthesized peptides. Two nascent polypeptide complex proteins (NAC proteins for short) were obtained through affinity purification, named α-NAC protein and β-NAC protein, respectively. Previously, based on the results of SDS-PAGE electrophoresis, the molecular weights of the two proteins were preliminarily determined to be 33KD and 22KD, respectively, but it was not until 2000 that the real molecular weights of the proteins were 23.492KD and 17.352KD through mass spectrometry analysis. The cDNA sequence of the NAC protein gene is shown in...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00A01G1/00A01G7/06
Inventor 符江华
Owner 北京东方先科科技有限公司
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