Surface material with protein adsorption resistant property and preparation method thereof

A technology of anti-protein adsorption and surface materials, which is applied in the field of surface materials and its preparation, to achieve the effects of broad application prospects, reliable principles, and low reaction costs

Inactive Publication Date: 2011-05-04
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the application of the principle of anti-protein adsorption to marine antifouling, and the direct chemical modification of zwitterions on the surface of organic silicon materials to form surface materials with anti-protein adsorption properties has not been reported yet.

Method used

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  • Surface material with protein adsorption resistant property and preparation method thereof
  • Surface material with protein adsorption resistant property and preparation method thereof
  • Surface material with protein adsorption resistant property and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Preparation of surface materials with anti-protein adsorption properties

[0012] (1) In a 250mL round bottom flask, add 2mmol triethylamine, 200mL tetrahydrofuran as solvent, add surface hydroxylated silicone matrix (surface area 1cm 2 , if the surface area increases, then increase the amount of each component in the experiment according to the corresponding proportion), seal the round bottom flask, place it in an ice-water bath, add an appropriate amount of 2-bromoisobutyryl bromide after stirring for 5 minutes, and seal the round bottom flask After 5 minutes, the round-bottomed flask was taken out from the ice-water bath, and the reaction was continuously stirred for 16 hours. After the reaction, the product was taken out from the glove box, washed with methanol and deionized water successively, and rinsed with N 2 Blow dry to obtain an intermediate containing an initiating group;

[0013] (2) The following reaction process is carried out in a glove box. ...

Embodiment 2

[0016] Example 2: Evaluation of anti-protein adsorption effect

[0017] Select bovine serum albumin (Mw=6KD) as the model protein to evaluate the anti-protein adsorption performance; Soak the No. 2 organic silicon surface material prepared in Example 1 in the bovine serum albumin phosphate buffer solution of 5mL 1g / L, under normal temperature After standing for 8 hours, the sample was taken out, rinsed with phosphate buffer solution, stained with Coomassie brilliant blue for 10 minutes, and tested for protein adsorption with a UV spectrophotometer. The number of moles of adsorbed protein is the amount of protein adsorption, and the protein adsorption inhibition rate is calculated according to the difference in the amount of protein adsorption on the surface of the material before and after modification, and the expression is as follows:

[0018]

[0019] Compared with the unmodified blank organosilicon base material, the adsorption inhibition rate of the surface zwitterion-...

Embodiment 3

[0020] Embodiment 3: Antifouling effect evaluation

[0021] The organosilicon material prepared in Example 1 was subjected to a comparative test of diatom adhesion inhibition in the laboratory, and Navicula smallis was selected as the experimental algae, and the organosilicon material was immersed in an initial concentration of 1×10 5 Each / mL navicula solution was cultured at 25°C for 7 days; the attachment amount of navicula was observed and counted under a three-dimensional video microscope (observing 10 visual fields to calculate the average number). The inhibition rate of diatom attachment was calculated according to the change of the attachment amount of Navicula microalgae on the surface of the material before and after adsorption, and the formula was as follows:

[0022]

[0023]The results of the diatom inhibition test show that the zwitterionic molecule-modified organosilicon surface material can increase the inhibition rate of diatoms by 75% compared with the orga...

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Abstract

The invention relates to a surface material with protein adsorption resistant property and a preparation method thereof, belonging to the technical field of biological materials. The surface material is an organic silicon surface material polymerized by monomer molecules with diionic property and organic silicon, has good protein adsorption resistant property and can be extensively applied to the fields of marine antifouling materials and biomedical materials. The surface material which has the protein adsorption resistant property through surface modification is prepared by carrying out graft polymerization on the monomer molecules with the diionic property on the surface of an organic silicon substrate; the surface material is combined with water molecules in a marine environment to form a water film on the surface of the material and can prevent the adsorption of proteins on the surface of the material because the water molecules contained in the water film are compactly arranged, thereby inhibiting the further adhesion of marine fouling organisms on the surface of the material and achieving the effect of effectively preventing and removing the fouling of marine organisms; in addition, the invention has wide application prospect.

Description

Technical field: [0001] The invention belongs to the technical field of new biomaterials, and relates to a surface material with anti-protein adsorption properties and a preparation method thereof. The surface material is an organic silicon surface material formed by polymerization of diionic monomer molecules and organic silicon. The anti-protein adsorption performance can be widely used in the fields of marine antifouling materials and biomedical materials. Background technique: [0002] There are a large number of marine organisms in seawater, and these marine organisms will adhere to the surface of ships and structures that have been in the seawater environment for a long time. This adhesion will increase the traveling resistance of the ship, seriously affect the sailing speed of the ship, increase fuel consumption, and at the same time damage the anti-corrosion coating on the surface of the ship and structures, thereby causing corrosion and damage on the surface of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/12C08J7/16C08L83/00C08L83/04
Inventor 许凤玲张金伟蔺存国
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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