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Application of immobilized quinone compound in accelerating denitrification process of microorganism

A compound and denitrification technology, which is applied in the fields of biological engineering, environmental engineering and chemical engineering, can solve the problems of staying in the laboratory and pilot test stage, complex process condition control, high equipment investment and operating costs, and reduce secondary Effects of pollution, low production costs, accelerated speed and capacity

Inactive Publication Date: 2012-05-23
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Claims
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AI Technical Summary

Problems solved by technology

However, most of the current biological denitrification treatment technologies still have two bottleneck problems: (1) The investment and operating costs of the process and equipment are relatively high; (2) The efficiency of biological denitrification is low
[0004] There are currently two types of solutions to improve the efficiency of biological denitrification. The first category is to use new biological denitrification mechanisms to research and develop new biological denitrification processes to improve the efficiency of nitrogen removal, such as short-range nitrification-denitrification, anaerobic ammonia Oxidation and simultaneous nitrification and denitrification, etc., but most of the actual engineering applications of these methods are only in the laboratory and pilot test stage, which affect many process parameters, and the control of process conditions is more complicated. It must be difficult; the second method is to improve the reaction rate of the organism itself to solve the efficiency problem, that is, to find special substances that promote the biological denitrification reaction, and to promote the redox rate in the biological denitrification process, which is called a redox mediator ( Redox Mediator, RM), using RM to promote biological nitrogen removal rate
[0005] In the past 20 years, studies have proved that some special substances containing quinone structure can accelerate the degradation of some pollutants. For example, the humic substances with quinone structure of Shewanella have played an important role in the decolorization of azo dyes; currently quinone compounds The research as a redox mediator is mainly in the degradation of refractory organics such as its catalytic azo dyes and some nitroaromatic compounds. According to foreign literature reports, sulfonic acid anthraquinone (anthraquinone-2-sulfonic acid, 2, 7-disulfonic acid anthraquinone) can accelerate the reaction process of NO donor S-nitrosoglutathione (GSNO) to produce NO, but there is no report on the acceleration of microbial denitrification process by immobilized quinone compounds

Method used

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  • Application of immobilized quinone compound in accelerating denitrification process of microorganism
  • Application of immobilized quinone compound in accelerating denitrification process of microorganism
  • Application of immobilized quinone compound in accelerating denitrification process of microorganism

Examples

Experimental program
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Effect test

Embodiment 1

[0014] One, an immobilized quinone compound, it is prepared by following steps:

[0015] a Weigh sodium alginate and add it to deionized water or normal saline, dissolve it completely in a constant temperature water bath at 90°C, then cool to 35-45°C to make a sodium alginate aqueous solution with a concentration of 6% by mass;

[0016] b Add 1,8-dichloroanthraquinone to the above solution and mix evenly, the weight of the 1,8-dichloroanthraquinone is 5% of the weight of the sodium alginate aqueous solution;

[0017] c Squeeze the mixed solution prepared in the above steps into the calcium chloride solution with a mass percentage concentration of 5% with a medical syringe, and the volume percentage of the mixed solution and the calcium chloride solution is 30%, forming a fixed diameter of 3 to 4mm. The pellets were thawed, cross-linked at room temperature for 5 h, and placed in a refrigerator at 4 °C. figure 1 It is the scanning electron micrograph of the immobilized pellet o...

Embodiment 2

[0028] One, an immobilized quinone compound, it is prepared by following steps:

[0029] a Weigh sodium alginate and add it to deionized water or normal saline, dissolve it completely in a constant temperature water bath at 90°C, then cool to 35~45°C to make a sodium alginate aqueous solution with a concentration of 3% by mass;

[0030] b Add an appropriate amount of 1,5-dichloroanthraquinone to the above solution and mix evenly, the weight of the 1,5-dichloroanthraquinone is 3% of the weight of the sodium alginate aqueous solution;

[0031]c Squeeze the mixed solution prepared in the above steps into the calcium chloride solution with a concentration of 3% by mass percentage with a syringe, and the volume percentage of the mixed solution and the calcium chloride solution is 50%, forming a fixed diameter of 3 to 4 mm. The pellets were thawed, cross-linked at room temperature for 5 h, and placed in a refrigerator at 4 °C for later use.

[0032] 2. The application of the immobi...

Embodiment 3

[0037] One, an immobilized quinone compound, it is prepared by following steps:

[0038] a Weigh sodium alginate and add it to deionized water or normal saline, dissolve it completely in a constant temperature water bath at 90°C, then cool to 35-45°C to make a sodium alginate aqueous solution with a concentration of 5% by mass;

[0039] b Add an appropriate amount of 1,4,5,8-tetrachloroanthraquinone to the above solution and mix evenly, the weight of the 1,4,5,8-tetrachloroanthraquinone is 4% of the weight of the sodium alginate aqueous solution;

[0040] c Squeeze the mixed solution prepared by the above steps into a calcium chloride solution with a mass percentage concentration of 2% with a medical syringe, and the volume percentage of the mixed solution and the calcium chloride solution is 40%, forming a 3-4mm diameter The beads were immobilized, cross-linked for 5 h at room temperature, and placed in a refrigerator at 4 °C for later use.

[0041] 2. Application of the imm...

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Abstract

The invention relates to application of an immobilized quinone compound in accelerating the denitrification process of a microorganism, belonging to the technical field of chemical engineering, biological engineering and environmental engineering. The immobilized quinone compound is 1,8-dichloroanthraquinone and 1,5-dichloroanthraquinone or 1,4,5,8-tetrachloroanthraquinone. The immobilized quinone compound can intensify, regulate and control the denitrification process of an organism, accelerate the velocity and the capacity of the microorganism on the denitrification process of nitrogenous substances, effectively reduce the cost of water treatment and has the advantages of good stability, loss difficulty and reduced secondary pollution, low price of immobilized raw material sodium alginate, good biocompatibility, simple fixing process and low production cost and is easy to realize redox mediator recovery, regeneration and recycling.

Description

technical field [0001] The invention relates to the application of an immobilized quinone compound in the process of accelerating microbial denitrification, and belongs to the technical fields of chemical engineering, biological engineering and environmental engineering. Background technique [0002] With the rapid development of industrial and agricultural production and the continuous growth of population, a large amount of untreated or improperly treated nitrogen-containing wastewater has seriously deteriorated the quality of the water environment and caused serious harm to human health and the survival of animals and plants. Nitrogen is the main factor causing eutrophication of water bodies. When a large amount of sewage containing nitrogen nutrients is directly discharged into the receiving water body, it will lead to abnormal growth of algae and other aquatic plants, consume dissolved oxygen in the water, and cause water quality deterioration and aquatic ecology. Destr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/28B01J31/02
Inventor 郭建博廉静杨景亮康丽李再兴刘春岳琳罗晓
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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