Extraction method of Diosbulbin B

Abstract

The invention discloses an extraction method of Diosbulbin B, which comprises the following steps: carrying out reflux extraction on airpotato yam acetone, and concentrating the extracting solution until no acetone smell emits, thereby obtaining an extract; mixing the extract and silica gel, adding petroleum ether for extraction, drying filter residues by evaporation, adding chloroform for extraction, and drying the extracting solution by evaporation, thereby obtaining a chloroform part; mixing the chloroform part and silica gel, passing the mixture through columns, eluting with a mixed solution of chloroform and methanol in the volume ratio of 98:2, and collecting the eluent at multiple sections; recovering the solvent from the eluent, dissolving in acetone, crystallizing to obtain a crystal, passing the filtrate through columns, and eluting with a mixed solution of chloroform and methanol in the volume ratio of 99:1; and recovering the solvent from the eluent, dissolving in acetone, crystallizing, and merging the crystals to obtain the Diosbulbin B. In the invention, by selecting the extraction process and the eluents, the Diosbulbin B can be extracted, and the obtained extract does not contain other substances, thus further separation and purification are not needed. The method is relatively simple to operate, can be used for effectively extracting the Diosbulbin B, and has important meanings for further research on pharmacology and drug effects of the Diosbulbin B.

Description

[0001] technical field

[0002] The invention relates to a method for extracting effective components of traditional Chinese medicine, in particular to a method for extracting the active component of xanthophyllum chinensis - lutein B, which belongs to the technical field of traditional Chinese medicine extraction. Background technique

[0003] Huangyaozi is a plant of the genus Dioscorea in the Dioscoreaceae family Dioscorea Bulbifera The dry tubers of L., also known as xanthophyll, huangdu, xanthum, xanthate root, xanthate fat, Shanzi mushroom, etc., are widely distributed in the world. Our country is mainly produced in Hubei, Hunan, Jiangsu and other places, while Henan, It is also produced in Shandong, Zhejiang, Anhui, Fujian and other provinces. It is cool in nature and bitter in taste, and has the effects of dispelling stagnation and eliminating gall, clearing heat and detoxifying, cooling blood and reducing fire. Xanthophyll contains diosbulbine A (diosbulbine A), s...