Extraction method of Diosbulbin B

An extraction method, the technology of yellow alone element, applied in the field of extracting the active ingredient of traditional Chinese medicine, the active ingredient of xanthate seed - yellow alone element B, can solve the problem of few and rare extraction methods, and achieve the effect of simple operation and high purity

Inactive Publication Date: 2011-05-25
SHANDONG ACAD OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, there are few methods for extracting the effective components of Xanthophylla chinensis, and the method for extracting Xanthophyllin B alone is even rarer. Therefore, finding a simple and effective method for extracting Xanthophyllin B is of great significance for more in-depth research on its pharmacological effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 1.75 kg of xanthophyll medicinal material, add 5 times the amount of acetone, heat and reflux in a water bath to extract twice, each time for 2 hours, filter the extract, combine and concentrate the filtrate until there is no acetone smell, and obtain 47 g of extract, add an equal amount of silica gel to mix the sample, 50 °C and dried under reduced pressure. Add 3 times the amount of petroleum ether to the dried silica gel sample, heat and reflux in a water bath to extract 3 times, each time for 1 hour, and filter the extract. Evaporate the filter residue to dryness under reduced pressure, add 6 times the amount of chloroform, heat and reflux for extraction 3 times, each time for 2 hours, filter the extract, combine the filtrates and evaporate to dryness to obtain 10 g of chloroform, heat and dissolve with a small amount of 95 ethanol, add an equal amount of column layer Analyze silica gel samples. Put the chloroform part on a silica gel column (4cm×13cm), and ch...

Embodiment 2

[0030] Take 1.75 kg of Xanthophyllum medicinal material, add 7 times the amount of acetone, heat and reflux in a water bath to extract twice, each time for 2 hours, filter the extract, combine and concentrate the filtrate until there is no acetone smell, and obtain an extract, add an equal amount of silica gel to the extract and mix the sample, Dry at 50°C under reduced pressure. Add 2 times the amount of petroleum ether to the dried silica gel sample, heat and reflux in a water bath to extract 3 times, each time for 2 hours, and filter the extract. Evaporate the filter residue to dryness under reduced pressure, add 8 times the amount of chloroform, heat and reflux for extraction 3 times, 3 hours each time, filter the extract, combine the filtrates and evaporate to dryness to obtain the chloroform part, heat and dissolve with a small amount of ethanol, add an equal amount of column chromatography silica gel Sample mixing. Put the chloroform part on a silica gel column (4cm×13...

Embodiment 3

[0033] The same method as in Example 1 is used to extract lutein B. The difference is that the amounts of various extraction solvents used are different. Specifically, the amount of acetone added is 4 times the mass of Xanthophyll, and 5 times the amount of petroleum oil is added to the dried silica gel sample. Ether extraction, adding 5 times the mass of chloroform to the extracted filter residue. The obtained crystal mass is 1.51kg, and all of them will be detected as lutein B.

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Abstract

The invention discloses an extraction method of Diosbulbin B, which comprises the following steps: carrying out reflux extraction on airpotato yam acetone, and concentrating the extracting solution until no acetone smell emits, thereby obtaining an extract; mixing the extract and silica gel, adding petroleum ether for extraction, drying filter residues by evaporation, adding chloroform for extraction, and drying the extracting solution by evaporation, thereby obtaining a chloroform part; mixing the chloroform part and silica gel, passing the mixture through columns, eluting with a mixed solution of chloroform and methanol in the volume ratio of 98:2, and collecting the eluent at multiple sections; recovering the solvent from the eluent, dissolving in acetone, crystallizing to obtain a crystal, passing the filtrate through columns, and eluting with a mixed solution of chloroform and methanol in the volume ratio of 99:1; and recovering the solvent from the eluent, dissolving in acetone, crystallizing, and merging the crystals to obtain the Diosbulbin B. In the invention, by selecting the extraction process and the eluents, the Diosbulbin B can be extracted, and the obtained extract does not contain other substances, thus further separation and purification are not needed. The method is relatively simple to operate, can be used for effectively extracting the Diosbulbin B, and has important meanings for further research on pharmacology and drug effects of the Diosbulbin B.

Description

[0001] technical field [0002] The invention relates to a method for extracting effective components of traditional Chinese medicine, in particular to a method for extracting the active component of xanthophyllum chinensis - lutein B, which belongs to the technical field of traditional Chinese medicine extraction. Background technique [0003] Huangyaozi is a plant of the genus Dioscorea in the Dioscoreaceae family Dioscorea Bulbifera The dry tubers of L., also known as xanthophyll, huangdu, xanthum, xanthate root, xanthate fat, Shanzi mushroom, etc., are widely distributed in the world. Our country is mainly produced in Hubei, Hunan, Jiangsu and other places, while Henan, It is also produced in Shandong, Zhejiang, Anhui, Fujian and other provinces. It is cool in nature and bitter in taste, and has the effects of dispelling stagnation and eliminating gall, clearing heat and detoxifying, cooling blood and reducing fire. Xanthophyll contains diosbulbine A (diosbulbine A), s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/20
Inventor 闫雪生于蓓蓓
Owner SHANDONG ACAD OF CHINESE MEDICINE
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