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Extract for treating rheumatoid arthritis

A rheumatoid, extractive technology, applied in the field of extracts of ethnic medicine Clematis unifolium, can solve the problems of quantitative control of drug quality without detection indicators, loss of active ingredients, insufficient effect, etc., and achieves convenient administration and dosage. Small and easy to detect

Inactive Publication Date: 2011-06-01
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the folk, clematis unifoliate is usually directly decocted with raw medicine, and the dosage is large, the active ingredients are seriously lost, and the effect is not good enough.
The extraction method is not included in the local standards, and there is no detection index to quantitatively control the quality of the drug

Method used

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  • Extract for treating rheumatoid arthritis
  • Extract for treating rheumatoid arthritis
  • Extract for treating rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dried clematis rhizomes are crushed, passed through an 8-mesh sieve, weighed 20g of the powder, and extracted twice with 80% ethanol as the extraction solvent, the first dosage is 200mL, the second dosage is 160mL, and the microwave power is 300W , the extraction time is 4min. After extraction, filter while hot, combine the filtrates, recover ethanol until there is no alcohol smell, transfer the residual liquid to a 100mL volumetric flask, add distilled water to the mark, and obtain a concentration of 0.2g crude drug·mL -1 the initial extract.

[0021] Accurately weigh 10 g (V=28 ml) of the pretreated D-101 macroporous resin, and wet-pack the column. Take 50ml of the above initial extract, add 0.5% dilute hydrochloric acid to adjust the pH value to 5, load the sample at a flow rate of 1BV / h, elute impurities at a flow rate of 4BV distilled water at 2BV / h, wash with 4BV 70% ethanol at a flow rate of 2BV / h The total saponins were removed, the eluate was collected, the s...

Embodiment 2

[0023] Dried clematis rhizomes were crushed, passed through an 8-mesh sieve, weighed 10 kg of powder, and extracted 3 times with 80% ethanol as the extraction solvent, the first dosage was 10 times the amount, and the second dosage was 8 Double the amount, the third dosage is 8 times the amount, the microwave power is 800W, and the extraction time is 10min. After extraction, filter while hot, combine the filtrates, recover ethanol until there is no alcohol smell, add distilled water to the residual liquid to 35L, and obtain the initial extract.

[0024] AB-8 macroporous resin was pretreated and packed into the column. Take the above initial extract, add 0.5% dilute hydrochloric acid to adjust the pH value to 5, load the sample at a flow rate of 2BV / h, elute impurities at a flow rate of 4BV distilled water at 1BV / h, and elute with 4BV 70% ethanol at a flow rate of 2BV / h Total saponins, collect the eluate, recover the solvent under reduced pressure, and vacuum-dry to obtain a b...

Embodiment 3

[0026] 1 material

[0027] 1.1 Animals

[0028] SPF grade SD rats, female, body weight 180-200g. Purchased from Hunan Slake Jingda Experimental Animal Co., Ltd., production license number SCXK (Xiangjiang) 2009-0009. The temperature in the laboratory is controlled at 23-25°C, the humidity is 60-70%, and the exhaust and ventilation are regularly performed. Experimental animal use license number: SYXK (Xiang) 2009-0002.

[0029] 1.2 Drugs and reagents

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Abstract

The invention relates to an extract of traditional Chinese medicine unifoliate clematis and an application of the extract as a medicine for treating rheumatoid arthritis. The extract is a unifoliate clematis extract which takes the traditional Chinese medicine unifoliate clematis as a raw material, is produced under certain technological conditions and contains various triterpenoid saponin components. The pharmacological experiments of animal general inflammation and immune inflammation models prove that the extract provided by the invention has an obvious treatment effect and has an important value for treating rheumatoid arthritis.

Description

technical field [0001] The invention relates to the extract of the ethnic medicine Clematis unifolia and its use as a medicine for treating rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is a multisystemic inflammatory autoimmune disease mainly involving peripheral joints. It belongs to the category of arthralgia syndrome in traditional Chinese medicine, and is similar to "calendar disease", "white tiger calendar festival" and "stubborn numbness". The main pathological changes are the infiltration of inflammatory cells in the joint synovium, the formation of pannus, and the erosion of cartilage and bone tissue, resulting in joint destruction. Without regular treatment, the condition will gradually develop, eventually leading to joint deformity, loss of function, and a high morbidity rate. The disease is distributed globally. The prevalence rate in my country is 0.32-0.36%, and that of Caucasians in European and American countries is about 1%....

Claims

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Application Information

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IPC IPC(8): A61K36/716A61P19/02A61P19/04A61P29/00
Inventor 李焕德向大雄刘艺平方平飞唐芳
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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