Preparation method of organic manganese enzyme preparation

Abstract

The invention provides a preparation method of an organic manganese enzyme preparation, which comprises the steps of: uniformly mixing raw materials, such as wheat bran, bean pulp, rice husk powder, a manganese sulfate solution and the like to prepare a mixture, carrying out high-temperature high-pressure sterilization, inoculating a koji tray strain of candida utilis, uniformly stirring and then culturing, then taking materials obtained by the culturing out, inoculating a koji tray strain of aspergillus usamii, uniformly stirring and then subculturing, then taking materials obtained by the subculturing out, inoculating a koji tray strain of bacillus subtilis to the materials obtained by the subculturing, uniformly stirring and then culturing for the third time, and drying and crushing materials obtained by the third-time culturing to prepare the organic manganese enzyme preparation. The method has the advantages of simple preparation process and low production cost; three times of transformation are continually carried out by adopting the three stains, and inorganic manganese is transformed into organic manganese with high transformation rate reaching above 98 percent; and the prepared enzyme preparation integrates organic trace elements, an enzyme preparation and probiotics into a whole and has multiple biological functions, simple production process and low production cost.

Description

technical field

[0001] The invention provides a preparation method of organomanganese enzyme preparation. Background technique

[0002] Manganese is an indispensable trace element for organisms. It is firstly a component of many enzymes. Therefore, manganese is closely related to certain metabolisms in the process of life activities, such as lipid metabolism and carbohydrate metabolism. In addition, manganese is crucial to the formation and growth of bones and to the reproductive function of higher animals. At present, most of the manganese products sold in the feed additive market are inorganic manganese, namely manganese sulfate, manganese chloride, manganese carbonate and manganese oxide. The potency is low when used, and long-term use will pollute water quality and the environment. Although there is a part of organic manganese, most of them are industrially synthesized products, the production process is complex, the cost is high, and the price is relatively expensive. ...

Examples

Embodiment 1

[0009] A method for preparing an organomanganese enzyme preparation, the method is as follows: 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 part by weight of rice husk powder, 0.6 part by weight of ammonium sulfate, 0.4 part by weight of potassium dihydrogen phosphate, malt 4 parts by weight, the ratio of 110 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.3% is mixed with the above-mentioned materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. Make its water content be 65% by weight, then, under the condition that pressure is 0.18MPa, temperature is 125 ℃, carry out high temperature and high pressure sterilization for 43 minutes, after sterilization, when above-mentioned material is cooled to 31 ℃, inoculate 2.3% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at a...

Embodiment 2

[0011] A method for preparing an organomanganese enzyme preparation, the method is as follows: 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, 0.6 parts by weight of potassium dihydrogen phosphate, malt 2 parts by weight, the proportioning of 90 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.5% is mixed with the above-mentioned materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water, Make its water content be 55% by weight, then, under the condition that pressure is 0.22MPa, temperature is 115 ℃, carry out high temperature and high pressure sterilization for 46 minutes, after sterilization, when above-mentioned material is cooled to 29 ℃, inoculate 2.7% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and culti...

Embodiment 3

[0013] A method for preparing an organomanganese enzyme preparation, the method is as follows: 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 part by weight of ammonium sulfate, 0.5 part by weight of potassium dihydrogen phosphate, malt 3 parts by weight, the proportioning of 100 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.4% is mixed with the above materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water, Make its water content be 60% by weight, then, under the condition that pressure is 0.20MPa, temperature is 120 ℃, carry out the high temperature and high pressure sterilization of 44 minutes, after sterilization, when above-mentioned material is cooled to 30 ℃, inoculate 2.5% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at ...

technical field

[0001] The invention provides a preparation method of organomanganese enzyme preparation. Background technique

[0002] Manganese is an indispensable trace element for organisms. It is firstly a component of many enzymes. Therefore, manganese is closely related to certain metabolisms in the process of life activities, such as lipid metabolism and carbohydrate metabolism. In addition, manganese is crucial to the formation and growth of bones and to the reproductive function of higher animals. At present, most of the manganese products sold in the feed additive market are inorganic manganese, namely manganese sulfate, manganese chloride, manganese carbonate and manganese oxide. The potency is low when used, and long-term use will pollute water quality and the environment. Although there is a part of organic manganese, most of them are industrially synthesized products, the production process is complex, the cost is high, and the price is relatively expensive. ...

Examples

Embodiment 1

[0009] A method for preparing an organomanganese enzyme preparation, the method is as follows: 81 parts by weight of bran, 13 parts by weight of soybean meal, 0.9 part by weight of rice husk powder, 0.6 part by weight of ammonium sulfate, 0.4 part by weight of potassium dihydrogen phosphate, malt 4 parts by weight, the ratio of 110 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.3% is mixed with the above-mentioned materials to obtain a mixture. After stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water. Make its water content be 65% by weight, then, under the condition that pressure is 0.18MPa, temperature is 125 ℃, carry out high temperature and high pressure sterilization for 43 minutes, after sterilization, when above-mentioned material is cooled to 31 ℃, inoculate 2.3% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at a...

Embodiment 2

[0011] A method for preparing an organomanganese enzyme preparation, the method is as follows: 85 parts by weight of bran, 11 parts by weight of soybean meal, 1.1 parts by weight of rice husk powder, 0.4 parts by weight of ammonium sulfate, 0.6 parts by weight of potassium dihydrogen phosphate, malt 2 parts by weight, the proportioning of 90 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.5% is mixed with the above-mentioned materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water, Make its water content be 55% by weight, then, under the condition that pressure is 0.22MPa, temperature is 115 ℃, carry out high temperature and high pressure sterilization for 46 minutes, after sterilization, when above-mentioned material is cooled to 29 ℃, inoculate 2.7% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and culti...

Embodiment 3

[0013] A method for preparing an organomanganese enzyme preparation, the method is as follows: 83 parts by weight of bran, 12 parts by weight of soybean meal, 1.0 part by weight of rice husk powder, 0.5 part by weight of ammonium sulfate, 0.5 part by weight of potassium dihydrogen phosphate, malt 3 parts by weight, the proportioning of 100 parts by weight of manganese sulfate solution with a mass percentage concentration of 0.4% is mixed with the above materials to obtain a mixture, after stirring evenly, when the water content of the mixture is insufficient, add water to the mixture with sterile water, Make its water content be 60% by weight, then, under the condition that pressure is 0.20MPa, temperature is 120 ℃, carry out the high temperature and high pressure sterilization of 44 minutes, after sterilization, when above-mentioned material is cooled to 30 ℃, inoculate 2.5% of its weight of Candida utilis (Candida utilis) aspergillus strains, stirred evenly and cultivated at ...