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Specific magnetic resonance contrast agent for brain tumor and neurodegenerative diseases and preparation method thereof

A technology for neurodegenerative diseases and magnetic resonance contrast agents, which can be used in MRI/magnetic resonance imaging contrast agents, pharmaceutical formulations, preparations for in vivo tests, etc. Good contrast effect, low toxicity and side effects, improved sensitivity and specificity

Inactive Publication Date: 2011-06-15
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the preparation of raw monoclonal antibodies generally has disadvantages such as cumbersome preparation, long cycle time, and high price.
At the same time, the use of monoclonal antibodies may cause an immune response in the human body, resulting in anti-antibodies, thereby limiting repeated use

Method used

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  • Specific magnetic resonance contrast agent for brain tumor and neurodegenerative diseases and preparation method thereof
  • Specific magnetic resonance contrast agent for brain tumor and neurodegenerative diseases and preparation method thereof
  • Specific magnetic resonance contrast agent for brain tumor and neurodegenerative diseases and preparation method thereof

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Embodiment 1

[0054] A specific contrast agent for magnetic resonance brain lesions, which is a conjugate of lactoferrin and superparamagnetic iron oxide nanoparticles, namely Lactoferrin-SPIO, with a particle size of about 13 nanometers.

Embodiment 2

[0056] A method for preparing the magnetic resonance brain lesion specific contrast agent described in embodiment 1, it may further comprise the steps:

[0057] (1) Prepare activated superparamagnetic iron oxide nanoparticle suspension: get superparamagnetic iron oxide nanoparticle, disperse in 1mol / L sodium acetate buffer solution of pH=5.0, add EDC and NHS (final concentration is respectively 5mM and 12.5mM), shake for 15 minutes, remove unreacted EDC and NHS with a magnet, then add 0.1mol / L PBS buffer solution with pH=7.4 to redisperse superparamagnetic iron oxide nanoparticles to obtain activated superparamagnetic Iron oxide nanoparticle suspension.

[0058] (2) Preparation of lactoferrin solution: Dissolve lactoferrin in 0.1 mol / L PBS buffer solution with pH=7.4.

[0059] (3) Add ② into ①, the reaction ratio is 0.1mg: 1mg, and react overnight at room temperature to obtain the reacted product.

[0060] (4) Separation: take out ③, remove unreacted lactoferrin with a magne...

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Abstract

The invention provides a contrast agent capable of specifically imaging tumor and neurodegenerative diseases (including Alzheimer's disease (AD), Parkinson's disease (PD) and the like) in magnetic resonance imaging and a preparation method thereof by using the high affinity between lactoferrin and cells which express a lactoferrin receptor on surface to realize target-directed imaging. The magnetic resonance contrast agent is formed by conjugating super-paramagnetic ferric oxide nanoparticles with lactoferrin, the particle size of the magnetic resonance contrast agent is below 20 nanometers, and the conjugate has the characteristics of small particle size, no toxicity, high specificity, high selectivity, high contrast effect, tissue concentration enough for in-vivo imaging, and the like.

Description

technical field [0001] The invention relates to medical detection reagent technology, in particular to a brain tumor and neurodegeneration-specific contrast agent for MRI (magnetic resonance imaging) contrast enhancement and a preparation method thereof. Background technique [0002] Magnetic resonance imaging is a new type of high-tech imaging examination method in recent years, and it is a new technology of medical imaging diagnosis that was only applied in clinic in the early 1980s. In clinical MRI detection, more than 30% of the diagnoses need to use MRI contrast agent (MRI contrast agent) to improve the imaging contrast, so that the tissue structure that lacks contrast difference can be displayed more clearly, so as to better display the tissues and organs in the body The structure and nature and functional status of lesions can greatly improve the accuracy and early diagnosis. Contrast agents are divided into paramagnetic and superparamagnetic contrast agents accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/06A61K49/14
Inventor 杨祥良朱艳红徐海波谢辉杨海徐辉碧赵彦兵蒋玮丽尹明媛
Owner HUAZHONG UNIV OF SCI & TECH
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