Method for measuring roxithromycin

A technology of roxithromycin and roxithromycin tablets, which is applied in the direction of color/spectral characteristic measurement and analysis through chemical reaction of materials, can solve the problems that the determination of roxithromycin has not been reported yet, and reach the instrument price Inexpensive, good selectivity, high sensitivity

Active Publication Date: 2013-07-24
山西德元堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ethyl violet is a reagent with a large molar absorptance coefficient, and it is widely used in analytical chemistry, but the method for determining roxithromycin based on the ethyl violet charge-transfer reaction spectrophotometry has not been reported yet.

Method used

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  • Method for measuring roxithromycin
  • Method for measuring roxithromycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Add 0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0mL 0.52mg / L roxithromycin solution in turn to 10mL colorimetric tube, 1.5mL 5.067×10 -4 mol / L ethyl violet solution, then add 0.5mL 0.125mol / L HCl solution, dilute to the mark with twice distilled water, and shake well. Heat it in a water bath at 50°C for 10 minutes, take it out and cool it to room temperature, measure the absorbance of the roxithromycin solution at a wavelength of 637nm with a 1cm cuvette on a UV-visible spectrophotometer, using twice distilled water as a reference Value A 637nm and the absorbance value A of the reagent blank solution without roxithromycin 0 , to calculate the absorbance difference ΔA 637nm =A 637nm -A 0 . Its absorbance difference ΔA 637nm The linear regression equation with roxithromycin concentration C is ΔA 637nm =3.9904C+0.0065, correlation coefficient 0.9990, apparent molar absorptivity ε 637 =3.34×10 6 L·mol -1 cm -1 . Take another two roxithromycin tablets (marked amount 150mg / ta...

Embodiment 2

[0014] Add 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0mL 0.52mg / L roxithromycin solution in turn to 10mL colorimetric tube, 1.5mL 5.067×10 -4 mol / L ethyl violet solution, then add 0.5mL0.125mol / L HCl solution, dilute to the mark with twice distilled water, and shake well. Heat it in a water bath at 50°C for 10 minutes, take it out and cool it to room temperature, measure the absorbance of the roxithromycin solution at a wavelength of 637nm with a 1cm cuvette on a UV-visible spectrophotometer, using twice distilled water as a reference Value A 637nm and the absorbance value A of the reagent blank solution without roxithromycin 0 , to calculate the absorbance difference ΔA 637nm =A 637nm -A 0 , the absorbance difference ΔA 637nm The linear regression equation with roxithromycin concentration c is ΔA 637nm =3.9904c+0.0065, correlation coefficient 0.9990, apparent molar absorptivity ε 637 =3.34×10 6 L·mol -1 cm -1 . Take another 3 capsules of roxithromycin capsules (marked amount 1...

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Abstract

The invention discloses a method for measuring roxithromycin, which comprises the steps of: adding 0.0mL, 0.5mL, 1.0mL, 1.5mL, 2.0mL, 2.5mL and 3.0mL of 0.50-0.55mg / L of roxithromycin solution, 0.5-2.5mL of 4.8-5.2*10-4mol / L ethyl violet solution and 0.2-1.0mL of 0.09-0.15mol / L HCI solution in a colorimetric cylinder of 10mL in sequence; diluting the mixed solution to a scale with secondary distilled water, and shaking uniformly; heating the solution in a water bath at the temperature of 45 to 55 DEG C for 10 minutes, taking out and cooling the solution to the room temperature, measuring the absorbance value A637nm of the mixed solution with the roxithromycin solution and the absorbance value A0 of a reagent blank solution without the roxithromycin at the wavelength of 637nm on an ultraviolet and visible spectrophotometer by using a cuvette of 1cm and taking second distillated water as a reference, and calculating the absorbance difference deltaA637nm which equals to A637nm minus A0; and taking another 2 to 10 roxithromycin tablets or roxithromycin capsules, grinding and mixing uniformly, then weighing 0.0050-0.0250g of the mixture, dissolving the weighed mixture with ethanol and metering the volume, and measuring the absorbance value by the same method. The measuring method has high sensitivity, is simple and convenient to operate, is fast, and has low cost of instruments.

Description

technical field [0001] The invention relates to a method for determining roxithromycin, in particular to a method for determining roxithromycin based on ethyl violet charge transfer reaction photometry. Background technique [0002] Roxithromycin, a derivative of erythromycin A, is a relatively new semi-synthetic macrolide antibiotic, which retains the original antibacterial activity and bactericidal mechanism of erythromycin, and improves chemical stability and affinity. Lipid, since its inception has been clinical broad-spectrum antibacterial drugs, against Gram-positive bacteria, including Staphylococcus aureus, Streptococcus pyogenes, Staphylococcus epidermidis, Streptococcus pneumoniae and some Gram-negative bacteria and anaerobic bacteria , Mycoplasma, Chlamydia, and Legionella all have strong antibacterial activity. Roxithromycin has the characteristics of small dosage, fast absorption and long acting time, etc. Its adverse reactions include common nausea, abdominal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N21/75
Inventor 唐宁莉蒙兴龙夏海鸣邓志业
Owner 山西德元堂药业有限公司
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