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Preparation method of alga-lysing burden-alleviating compound biological agent

A technology of biological preparations and compound biological bacteria, which is applied in chemical instruments and methods, biological water/sewage treatment, fungi, etc., can solve the problems of secondary pollution of water bodies, achieve ecological balance, be easy to cultivate, and eliminate environmental stench Effect

Active Publication Date: 2014-03-12
武汉昌宝环保工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical algae removal technology has the advantages of fast and efficient, it will cause secondary pollution of water bodies, and its direct application in lakes and reservoirs as drinking water sources is limited or must be used with caution

Method used

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  • Preparation method of alga-lysing burden-alleviating compound biological agent

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] Ⅰ, Ⅱ grade bacteria (laboratory) cultivation:

[0053] Strains are divided into parent species (Class I species) and original species (Class II species).

[0054] Class I strains: produced by propagating pure mycelium or spores in test tube culture medium. It can reproduce original strains and is also suitable for strain preservation.

[0055] Class II strains: the mother mycelium is propagated on a solid medium. This process enhances the adaptability of the mycelium to the culture environment and plays a role in propagation.

[0056] 1) Medium:

[0057] ① Algae-lysing culture medium:

[0058] Beef extract 1-5g peptone 8-12g sodium chloride 3-7g water 1000ml agar 1.5-2% pH= pH=7.0-7.8

[0059] ② Load-reducing compound bacterial culture medium

[0060] Brown sugar 80-120g dipotassium hydrogen phosphate 0.01-0.05g magnesium sulfate and 0.01-0.03g water 1000ml pH=6.8-7.4

[0061] A preparation method for algae-dissolving and burden-reducing compound biologi...

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Abstract

The invention discloses a preparation method of an alga-lysing burden-alleviating compound biological agent, which comprises the following steps: A, culture of level-I and level-II strains: (1) preparing a culture medium; (2) culturing level-I seeds; and (3) culturing level-II seeds; B, culture of level-III seeds: (1) culturing level-III seeds of alga-lysing bacteria; and (2) culturing level-III seeds of burden-alleviating compound agents; and C, large-scale production: (1) preparing a culture medium; (2) carrying out large-scale fermentation production of alga-lysing bacteria; (3) carrying out large-scale fermentation production of burden-alleviating compound agents; and (4) preparing the alga-lysing burden-alleviating compound biological agent, and packaging. The method is feasible, is convenient to operate, reduces the eutrophic water body and controls the outbreak of the blue-alga bloom. The alga-lysing burden-alleviating compound biological agent is easy to culture, has low cost, is suitable for factory production, does not pollute the water body and has environmental and social benefits.

Description

technical field [0001] The invention belongs to the field of environmental protection bioengineering, and more specifically relates to a method for preparing algae-dissolving and burden-reducing composite biological bacterial agent. The bacterial agent is easy to cultivate, is suitable for industrial production, and is suitable for reducing eutrophic water body and controlling the outbreak of cyanobacteria bloom. Background technique [0002] Cyanobacteria are the earliest photoautotrophic organisms that appeared on the earth. During the long-term evolution process, these organisms have developed a set of unique morphology and physiological and biochemical mechanisms, which can reproduce in various habitats, making them have certain competition Advantage. When the environmental conditions are suitable, some cyanobacteria can grow rapidly. When a certain biomass is reached, these algae gather in large numbers on the surface of the water body, forming algae aggregates visibl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34
Inventor 刘浩唐智伟刘军刘彬彬赵冰项娇
Owner 武汉昌宝环保工程有限公司
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