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Kanamycin extracting process

A kanamycin and extraction process technology, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., to reduce the possibility of adverse reactions, have far-reaching social significance, and improve the effect of treatment effects

Active Publication Date: 2011-07-20
山东齐发药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a kanamycin extraction process to solve the problems existing in the existing production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kanamycin extraction process, comprising the following steps:

[0030] (1) Collection of active ingredients:

[0031] The type of adsorption column used is Φ50×300mm, filled with 500ml CD180 resin.

[0032] Take the crystallization mother liquor and recover the waste water after ethanol, lower it to normal temperature, adjust the pH to 7.5 with HCL, measure the potency to be 7454U / ml, pass it into a resin column equipped with CD180 resin at a flow rate of 7ml / min for dynamic adsorption, and use thin layer chromatography The composition of the analytical solution was detected by the method. After 105 hours of adsorption, a large amount of impurities and KA and a small amount of KB began to leak, and the adsorption was stopped. The adsorption volume was 44.1 liters, 0.33 billion. Wash the adsorption resin with water until no Cl ions are detected by silver nitrate detection.

[0033] Use ammonia water with a concentration of 2mol / L to analyze at a flow rate of 7ml / min....

Embodiment 2

[0041] A kanamycin extraction process, comprising the following steps:

[0042] (1) Collection of active ingredients:

[0043] The type of adsorption column used is Φ50×300mm, filled with 500ml CD180 resin.

[0044] Take the crystallization mother liquor to recover the waste water after ethanol, lower it to normal temperature, adjust the pH to 6.5 with HCL, and measure the potency to be 18605U / ml, pass it into a resin column equipped with CD180 resin at a flow rate of 2ml / min for dynamic adsorption, and use thin layer chromatography The composition of the analytical solution was detected by the method. When the adsorption was about 60 hours, a large amount of impurities, KA, and a small amount of KB began to leak, and the adsorption stopped. The adsorption volume was 17.2 liters, 0.32 billion. Wash the adsorption resin with water until no Cl ions are detected by silver nitrate detection.

[0045] Use ammonia water with a concentration of 2mol / L to analyze at a flow rate of 5...

Embodiment 3

[0053] A kanamycin extraction process, comprising the following steps:

[0054] (1) Collection of active ingredients:

[0055] The type of adsorption column used is Φ50×300mm, filled with 400ml CD180 resin.

[0056] Take the crystallization mother liquor and recover the waste water after ethanol, lower it to normal temperature, adjust the pH to 6.5 with HCL, and measure the potency to be 18939U / ml, pass it into a resin column equipped with CD180 resin at a flow rate of 2ml / min for dynamic adsorption, and use thin-layer chromatography The composition of the analytical solution was detected by the method. When the adsorption was about 65 hours, a large amount of impurities, KA, and a small amount of KB began to leak, and the adsorption stopped. The adsorption volume was 13.2 liters, 0.25 billion. Wash the adsorption resin with water until no Cl ions are detected by silver nitrate detection.

[0057] Use ammonia water with a concentration of 2mol / L to analyze at a flow rate of ...

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PUM

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Abstract

The invention relates to a kanamycin extracting process, which comprises: introducing waste water from recovery of ethanol from kanamycin crystallization mother liquor into a resin column at a speed of 7 million of kanamycin units per liter of resin per hour for dynamic absorption; collecting components; analyzing; collecting different kinds of analytical liquid in different time periods to separate different components in the waste water; and concentrating and crystallizing different kinds of analytical liquid to obtain the finished products of the different components.

Description

technical field [0001] The invention relates to the production field of biological fermentation products, in particular to a production process for extracting kanamycin from kanamycin wastewater. Background technique [0002] Kanamycin is an aminoglycoside antibiotic that has a strong antibacterial effect on most Gram-negative bacteria, Enterobacter, Proteus, Salmonella, Pasteurella multocida, etc. It is also effective against Staphylococcus aureus and Mycobacterium tuberculosis more sensitive. However, sensitive bacteria are prone to develop resistance to kanamycin, so kanamycin is no longer directly used in clinical practice, but after further processing, kanamycin monosulfate, amikacin sulfate and other products are produced. For clinical use. [0003] In the current kanamycin extraction process, in the final crystallization process, the crystallization is carried out by adding ethanol to the concentrated solution and then centrifuged. In addition to ethanol, there is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/234C07H1/06
Inventor 韩莉莉陈夏辉丁新仁赵国毅
Owner 山东齐发药业有限公司
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