Method of extracting active substances with high content of flavones from ginkgo leaves

A technology with high flavonoid content and active substances, which is applied in the field of extracting active substances with high flavonoid content, can solve the problems of low yield and many impurities in the extraction process of ginkgo biloba, achieve good adsorption-analysis effect, improve extraction rate, and low cost Effect

Inactive Publication Date: 2011-08-03
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at providing a new extraction method aiming at the shortcomings of the existing ginkgo leaf extraction process, such as low yield and many impurities, and the technical problem to be solved is to increase the content of flavonoids in active substances

Method used

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  • Method of extracting active substances with high content of flavones from ginkgo leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Ginkgo biloba powder preparation:

[0036] The dried ginkgo leaves are pulverized and passed through a 60-mesh sieve to obtain ginkgo leaf powder.

[0037] (2) Pretreatment of Ginkgo biloba:

[0038] Take 5 g of crushed ginkgo leaf powder, add 20 mL of calcium hydroxide solution with a pH of 8, and place it under a microwave power of 800 W for 3.0 min. The ginkgo leaf suspension after the microwave treatment is placed in a polyethylene bag and an equal volume of 90vt% ethanol solution is added to seal it. Place the sealed polyethylene bag under 300Mpa high pressure for 15 minutes to obtain the pretreatment solution, take it out, let it stand, and set it aside.

[0039] (3) Extraction of ginkgo biloba active substances:

[0040] Add 75vt% ethanol solution to the ginkgo biloba pretreatment solution, the liquid-to-material ratio (volume mL: mass g) is 20:1, adjust the pH to 8, heat and reflux for 60 minutes, let stand, filter, and reflux and extract the residue unde...

Embodiment 2

[0056] (1) Ginkgo biloba powder preparation:

[0057] The dried ginkgo leaves are pulverized and passed through a 60-mesh sieve to obtain ginkgo leaf powder.

[0058] (2) Pretreatment of Ginkgo biloba:

[0059] Take 5 g of crushed ginkgo leaf powder, add 20 mL of calcium hydroxide solution with a pH of 9, and place it under a microwave power of 800 W for 3.0 min. The ginkgo leaf suspension after the microwave treatment is placed in a polyethylene bag and an equal volume of 90vt% ethanol solution is added to seal it. Place the sealed polyethylene bag under 300Mpa high pressure for 15 minutes to obtain the pretreatment solution, take it out, let it stand, and set it aside.

[0060] (3) Extraction of ginkgo biloba active substances:

[0061] Add 75vt% ethanol solution to the ginkgo biloba pretreatment solution, the liquid-to-material ratio (volume mL: mass g) is 20:1, adjust the pH to 8, heat and reflux for 60 minutes, let stand, filter, and reflux and extract the residue unde...

Embodiment 3

[0077] (1) Ginkgo biloba powder preparation:

[0078] The dried ginkgo leaves are pulverized and passed through a 60-mesh sieve to obtain ginkgo leaf powder.

[0079] (2) Pretreatment of Ginkgo biloba:

[0080] Take 5 g of crushed ginkgo leaf powder, add 20 mL of calcium hydroxide solution with a pH of 10, and place it under a microwave power of 800 W for 3.0 min. The ginkgo leaf suspension after the microwave treatment is placed in a polyethylene bag and an equal volume of 90vt% ethanol solution is added to seal it. Place the sealed polyethylene bag under 300Mpa high pressure for 15 minutes to obtain the pretreatment solution, take it out, let it stand, and set it aside.

[0081] (3) Extraction of ginkgo biloba active substances:

[0082] Add 75vt% ethanol solution to the ginkgo biloba pretreatment solution, the liquid-to-material ratio (volume mL: mass g) is 20:1, adjust the pH to 8, heat and reflux for 60 minutes, let stand, filter, and reflux and extract the residue und...

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Abstract

The invention provides a method of extracting active substances with high content of flavones from ginkgo leaves, which comprises the following steps of: putting powder of the ginkgo leaves into a calcium hydroxide solution with the pH (potential of hydrogen) value of 8 to 10 to process for 1-3min through microwave, wherein the material to solution ratio of the mass of the powder of the ginkgo leaves to the volume of the calcium hydroxide solution is 1:(3-5); adding 65-95vt% of ethanol solution of equal volume into the solution after the microwave treatment, treating the solution for 15-25minat the pressure of 200-300MPa to obtain a pretreatment liquid after the solution is sealed through a flexible package; heating and refluxing the pretreatment liquid for at least two times under the condition that the pH value is 8 to 11 through the ethanol solution with the concentration of 65-95vt%, mixing filtrate and regulating the pH value of the filtrate to 7, and centrifuging and separatingthe filtrate to obtain a liquid supernatant; adding 0.3-0.5wt% of vitamin E into the liquid supernatant, and uniformly mixing the mixture to obtain a supernatant sample; and adsorbing and analyzing the supernatant sample through AB-8 type macroporous absorption resin, and separating the mixture to obtain an analytical liquid, and obtaining the active substances of the ginkgo leaves after the analytical liquid is concentrated and lyophilized in vacuum. The yield of the active substances is as high as 25%, wherein the content of the flavones is as high as 22%.

Description

1. Technical field [0001] The invention relates to a method for extracting active substances with high flavonoid content from ginkgo leaves. 2. Background technology [0002] Ginkgo bilobal (Ginkgo bilobal), also known as ginkgo tree, is a gymnosperm plant with a tree age of more than a thousand years. It is a relict plant in the ancient Permian period. It is known as a "living fossil" in the plant kingdom and is the oldest relict plant in the existing seed plants. Ginkgo biloba has many characteristics such as strong adaptability and strong reproductive ability. [0003] The medical and nutritional health effects of ginkgo fruit have long been known, but the development and utilization of ginkgo leaves is much later. Germany and France are the first countries engaged in the development of Ginkgo biloba products in the world. They first developed Ginkgo biloba leaves for the treatment of cardiovascular diseases in the mid-1970s. Since then, other countries have devoted them...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P3/06A61P7/02A61P9/10A61P25/00A61P29/00A61P37/08A61K127/00
Inventor 叶明倪林
Owner HEFEI UNIV OF TECH
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