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Bone tissue engineering scaffold material having anti-infection ability and preparation method of bone tissue engineering scaffold material

A technology for bone tissue engineering and scaffold materials, applied in the field of bone tissue engineering scaffold materials with anti-infection ability and its preparation, achieving excellent mechanical properties and biocompatibility, simple preparation method, and efficient sterilization

Active Publication Date: 2011-08-10
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the introduction of too much of these drugs will cause side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Weigh 0.1 g of dextran, dissolve it in 50 ml of deionized water, prepare a dextran solution with a mass concentration of 0.2%, and stir until it is completely dissolved.

[0023] (2) 6.8g silver nitrate (AgNO 3 ) was dissolved in 50ml dextran solution to form a concentration of 0.8M AgNO 3 - Dextran solution, which is then cooled in an ice bath for 10 minutes. Then add excess sodium borohydride therein and stir; stop stirring when the solution turns bright yellow; centrifuge, wash with water, and vacuum dry to obtain nano silver powder.

[0024] (3) 3.2g disodium hydrogen phosphate (Na 2 HPO 4 ) dissolved in dextran solution to form 0.5M Na 2 HPO 4 solution, to which 0.5M CaCl was added dropwise 2 solution, so that the mass ratio of Ca and P is 1.67, after fully stirring, use sodium hydroxide solution to adjust its pH>8.5, after stirring for 1-5 hours, after standing for 12-48 hours, freeze-drying to obtain hydroxyapatite powder.

[0025] (4) take by weighin...

Embodiment 2

[0030] (1) Weigh 0.2 g of dextran, dissolve it in 50 ml of deionized water, prepare a dextran solution with a mass concentration of 0.4%, and stir until it is completely dissolved.

[0031] (2) 3.4g silver nitrate (AgNO 3 ) was dissolved in 50ml dextran solution to form a concentration of 0.4M AgNO 3 - Dextran solution, then put it into an ice bath to cool for 10 minutes, then add excess sodium borohydride and stir it, stop stirring when the solution turns bright yellow, centrifuge, wash with water, and vacuum dry to obtain nano silver powder.

[0032] (3) 3.2g disodium hydrogen phosphate (Na 2 HPO 4 ) dissolved in dextran solution to form 0.5M Na 2 HPO 4 solution; to which 0.5M CaCl was added dropwise 2 Solution, the material ratio of Ca and P is 1.67, after fully stirring, adjust its pH>8.5 with sodium hydroxide solution, stir for 5 hours, stand still for 48 hours, freeze-dry to obtain hydroxyapatite powder.

[0033] (4) take by weighing 1.4g chitosan, be dissolved in...

Embodiment 3

[0038] (1) Weigh 0.3 g of dextran, dissolve it in 50 ml of deionized water to prepare a dextran solution with a mass concentration of 0.6%, and stir until it is completely dissolved.

[0039] (2) 0.085g silver nitrate (AgNO 3 ) was dissolved in 50ml dextran solution to form AgNO with a concentration of 0.01M 3 - Dextran solution, then put it into an ice bath to cool for 10 minutes, then add excess sodium borohydride and stir it, stop stirring when the solution turns bright yellow, centrifuge, wash with water, and vacuum dry to obtain nano silver powder.

[0040] (3) 3.2g disodium hydrogen phosphate (Na 2 HPO4 ) dissolved in dextran solution to form 0.5M Na 2 HPO 4 solution, to which 0.5M CaCl was added dropwise 2 Solution, so that the mass ratio of Ca and P is 1.67; after fully stirring, adjust its pH>8.5 with sodium hydroxide solution; after stirring for 1-5 hours, after standing for 12-48 hours, freeze-drying to obtain hydroxyapatite powder.

[0041] (4) take by weigh...

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Abstract

The invention discloses a bone tissue engineering scaffold material having anti-infection ability and a preparation method of the bone tissue engineering scaffold material, belonging to the technical field of the preparation of bone tissue engineering biomaterials. The bone tissue engineering scaffold material comprises nano-silver, hydroxylapatite, glucosan and chitosan. The simple preparation method of the invention comprises the steps of: preparing the nano-silver and the hydroxylapatite respectively on the presence of the glucosan, then dispersing the nano-silver and the hydroxylapatite to mixed solution of the glucosan and the chitosan, performing glutaraldehyde crosslinking on the mixed solution and placing the mixed solution in a low temperature refrigerator for prefreezing, and freezing and drying to obtain three-dimensional porous scaffold material. The bone tissue engineering scaffold material has a spatial structure appropriate for the growth of osteoblast and appropriate mechanical properties and degradation property, is also durable and efficient in sterilization and has excellent biocompatibility, thus bone tissue regeneration can be promoted.

Description

technical field [0001] The invention belongs to the technical field of preparation of bone tissue engineering biomaterials, and in particular relates to a bone tissue engineering scaffold material with anti-infection ability and a preparation method thereof. Background technique [0002] Bone tissue engineering strategy is one of the effective means to treat bone defects, especially large bone defects. When bone tissue engineering scaffold materials are implanted into the defect site, it is inevitable to have direct contact with the surrounding tissue, so after the operation, the infection is relatively serious, which affects the repair of the defect site. Therefore, biomaterials should have excellent antibacterial activity to reduce the possibility of infection and improve the long-term storage performance of scaffold materials. At present, it is often used to load antibacterial drugs (eg, gentamicin, cephalosporin antibacterial drugs) into bone tissue engineering scaffold...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/54A61L27/20A61L27/12
Inventor 王常勇李俊杰杨桂利郝彤
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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