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Method for preparing Raman sensor based on gold nanorod self-assembly under polymerase chain reaction

A gold nanorod, self-assembly technology, applied in biochemical equipment and methods, bioreactor/fermenter combination, bioreactor/fermenter for specific purposes, etc., can solve the problem of lack of effective methods for Raman sensors, etc. , to achieve the effect of enhancing Raman detection of nucleic acids

Inactive Publication Date: 2013-01-23
JIANGNAN UNIV +1
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Problems solved by technology

At present, the preparation of Raman sensors based on gold rod assembly still lacks an effective method, and the present invention fills the gap in this field

Method used

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  • Method for preparing Raman sensor based on gold nanorod self-assembly under polymerase chain reaction

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Embodiment Construction

[0020] The preparation of gold nanorods, the preparation of gold nanorods utilizes the gold seed growth method.

[0021] Synthesis of gold seeds: 2.5 mL of 0.0005 M chloroauric acid (HAuCl 4 ) was dissolved in 2.5mL of 0.2M cetyltrimethylammonium bromide (CTAB), mixed evenly, and 0.3mL of newly prepared, pre-cooled 0.01M sodium borohydride solution was quickly added to the above solution, And vigorously mixed for 2 minutes, then placed at room temperature 25 ° C for 2 hours, ready to use.

[0022] For the growth of gold nanorods, the configuration of the growth solution: 0.15mL, 0.004M AgNO 3 , 5mL, 0.001M HAuCl 4 Add the solution to 5mL, 0.2M CTAB, then add 70μL of 0.0788M Vc, fully reduce for 2min, add 12μL of the above-mentioned gold seeds, stir fully for 20s, and then stand at 25°C for use.

[0023] Directional modification of gold nanorods. The modification method is end surface modification, the synthesized gold rod, the concentration is 2nM, centrifuged at 10000r / mi...

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Abstract

The invention provides a method for preparing a Raman sensor based on gold nanorod self-assembly under a polymerase chain reaction, belonging to the technical field of preparations of nano materials and nucleic acid Raman sensors. The method mainly comprises the following implementation steps of: (1) preparation of gold nanorods; (2) growth of the gold nanorods; (3) directional modification of the gold nanorods; (4) a PCR assembling process; and (5) preparation of a nucleic acid Raman sensor based on the gold nanorod self-assembly. In the invention, the nucleic acid Raman sensor is prepared based on the end surface to end surface self-assembly of the gold nanorods, the self-assembly is carried out on the basis of the polymerase chain reaction and applied to a PCR (Polymerase Chain Reaction) system through primer directional modification of the end surfaces of the gold nanorods; and through amplification of the PCR on the surfaces of gold nanoparticles, the gold rods are assembled in acontrollable manner and used for achieving a purpose of detecting nucleic acid by being applied to surface enhanced Raman spectroscopy.

Description

technical field [0001] The invention discloses a method for preparing a nucleic acid Raman sensor based on gold nanorod end-face self-assembly. The self-assembly is based on polymerase chain reaction, and belongs to the technical field of nanomaterial and nucleic acid Raman sensor preparation. Background technique [0002] Due to the small size of nanomaterials (1-100 nm), electrons are confined to a very small space, and the wave function of electrons is restricted, showing many strange properties, namely its optics, heat, electricity, magnetism, mechanics and The chemical properties will be significantly different compared to bulk solids. The assembly of nanomaterials will have new aggregate properties based on the properties of individual nanomaterials. The assembly of nanomaterials has been a research hotspot that researchers have been paying attention to for a long time. The assembly of nanostructures using DNA as a template is an emerging research field developed in r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12M1/34C12Q1/60
Inventor 王利兵胥传来马伟匡华朱颖越陈伟徐丽广刘丽强赵媛
Owner JIANGNAN UNIV
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