Preparation method of photoresponsive intelligent gel microspheres for three-dimensional cell culture
A three-dimensional culture and smart gel technology, applied in animal cells, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve problems such as difficult to study a single process, difficult to study intermediate processes, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0025] Preparation of modified azobenzenes: carboxylation of 4-aminoazobenzene and its polymerization with arginine-glycine-aspartic acid (RGD) (Azo-RGD) (Reference: Z. Zhangetal. Effects of immobilizing sites of RGD peptides in amphiphilic block copolymers on of celladhesion.Biomaterials, 2010, 7873-7882): 1.97g of 4-aminoazobenzene and 1.20g of succinic anhydride were dissolved in 25mL of acetone, 0.97g of anhydrous pyridine was added, the reaction was heated at 60°C for 6h, filtered and vacuum Dry for 24 hours to prepare carboxylated 4-aminoazobenzene Azo-COOH.
[0026] Take 0.137g (0.46mmoL) of the prepared Azo-COOH, dissolve it in 100mL of dichloromethane, and dissolve 0.225g (1.17mmoL) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt Add salt (EDC) and 0.135g (1.17mmoL) N-hydroxysuccinimide (NHS) to the above solution, stir at room temperature for 24h, remove dichloromethane by rotary evaporation, add 10mL of acetonitrile to dissolve the polymer, and then Add 0.1...
Embodiment 1
[0028] Preparation method of light-responsive smart gel microspheres for three-dimensional cell culture:
[0029] (1) Preparation of aqueous phase solution: Take 0.7800g (5mmoL) poly(methyl vinyl ether-maleic anhydride) and 0.2270g (0.2mmoL) β-cyclodextrin, dissolve in 10mLN, N-dimethyl formaldehyde In amide (DMF), under the protection of nitrogen, heat the reaction at 80°C for 9 hours, and rotate to evaporate to remove the N,N-dimethylformamide solvent; 1.0000g (0.05mmoL) of polyethylene glycol (20000) and 0.0612g (0.2mmoL) of agarose (AG) was dissolved in 10mL of water, added to the above reactants, and shaken until dissolved to prepare an aqueous phase solution with a concentration of 0.2068g / mL for later use.
[0030] (2) Formation of microspheres: Weigh 50ml of simethicone and pour it into a 100ml tall beaker, heat to 80°C, and at a stirring speed of 1000rpm, use a dropper to dissolve 2.5mL of the aqueous phase solution prepared in step (2) Slowly drop into simethicone o...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com