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Acinetobacter sp. and application of composite bacterial agent of acinetobacter species

A compound bacterial agent and bacillus technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problems of imperfection, inability to decompose and utilize, and accelerate the decomposition of organic matter, etc., to achieve good results

Active Publication Date: 2012-07-04
浙江天科高新技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] When photosynthetic bacteria are placed in ponds, they can eliminate harmful substances such as ammonia nitrogen, hydrogen sulfide, and organic acids in the water body, improve water quality, and balance pH. Feed, plankton residues, etc. cannot be decomposed and utilized; however, such strains as Bacillus subtilis and Bacillus licheniformis can secrete a variety of extracellular enzymes to decompose macromolecular organic substances into small molecular organic substances, and then further mineralize them to form Inorganic salts speed up the decomposition of organic matter. At present, most farmers use methods such as splashing photosynthetic bacteria to deal with it, but in general, the products still have limitations and are not perfect.

Method used

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  • Acinetobacter sp. and application of composite bacterial agent of acinetobacter species
  • Acinetobacter sp. and application of composite bacterial agent of acinetobacter species
  • Acinetobacter sp. and application of composite bacterial agent of acinetobacter species

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Effect test

Embodiment 1

[0023] Collect water and sediment from eutrophication ponds, inoculate them into liquid basal medium, and cultivate them at 32-35°C with a shaker speed of 150r / min for 2 days. The ammonia nitrogen value in the water body is used as an indicator to significantly reduce ammonia nitrogen. The liquid culture mixed microorganisms were diluted and spread on the solid basic medium, the above operation was repeated twice, and a single colony with good growth was picked as the primary screening bacteria. Inoculate the primary screened strain in the liquid separation medium, and culture it for 3-4 days at 32-35°C with a shaker speed of 150r / min to measure the content of MC-LR and determine the ability of the strain to degrade MC-LR. Select the colony with obvious reduction of ammonia nitrogen and degrade MC-LR to a certain extent and purify twice to obtain the screened strain ZJTK-006.

[0024] Basal medium: Na 2 HPO 4 2.4g, KH 2 PO 4 2.6g, NaCl0.328g, MgSO 4 ·7H 2 O 0.5g, CaCl 2...

Embodiment 2

[0028]The present invention isolates a strain of Acinetobacter sp. ZJTK-006, and identifies it according to its morphological characteristics and phylogenetic analysis based on 16S rDNA sequence. It is characterized in that it is a pure strain isolated and purified from the bottom mud of eutrophic ponds, its 16SrDNA has the sequence of SEQIDNo.1, and it is stored in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, a preservation unit designated by the Chinese Patent Office. The number is: CGMCCNo.3452.

[0029] (1) Morphology and physicochemical characteristics of Acinetobacter sp. ZJTK-006

[0030] Morphological characteristics:

[0031] Brevibacterium subspheroidus, 0.5-1.0μm×0.6-1.6μm, no spores, negative Gram stain, occasionally difficult to decolorize, (see picture); the colony is milky white, round, with neat edges.

[0032] Physical and chemical characteristics:

[0033] The strain is obligately aerobic, positive ...

Embodiment 3

[0042] Bacillus subtilis culture conditions:

[0043] Medium: beef extract 10g / L, peptone 5g / L, NaCl10g / L, PH 7.2-7.5,

[0044] Culture temperature: 32-35 ℃ constant temperature shaking culture.

[0045] Culture conditions of Rhodopseudomonas palustris:

[0046] Medium: Sodium Propionate 1.0g, Ammonium Sulfate 1.0g, Potassium Dihydrogen Phosphate 0.5g, Dipotassium Hydrogen Phosphate 0.5g, Magnesium Sulfate 0.2g, Sodium Chloride 0.5g, Yeast Extract 0.1g, Water 1000ml, PH6.5 -7.2

[0047] Culture temperature: 33-35°C, light 3000Lx, static culture for 5-7 days.

[0048] Preparation of microbial compound bacterial agent:

[0049] The strains Acinetobacter sp. ZJTK-006, Bacillus cereus As. 1.1414, and Rhodopseudomonas palustris 1.2181 were inoculated in their respective media according to the media formula, and cultured separately to make The number of each bacteria ≥ 1.0×10 9 cfu / ml, mixed uniformly according to the volume ratio of the cultures at a volume ratio of 1:1:3 to ...

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Abstract

The invention discloses an acinetobacter sp. and application of a composite bacterial agent of the acinetobacter sp. The bacterial agent consists of acinetobacter sp. ZJTK-006, bacillus subtilis and rhodopseudomonas palustris, which are proportioned in volume ratio of 1:1:3 according to culture after being cultured to form the microbial composite bacterial agent, wherein the preservation number of the acinetobacter sp. ZJTK-006 is CGMCC No. 3452. The bacterial agent prepared by the invention is applicable to removal treatment of ammonia nitrogen and chlorophyll alpha in a eutrophication and high-density cultivation water body, so that a good microecological system is formed, and the ecological system restoration of the whole water body is promoted.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to an application of Acinetobacter and its composite bacterial agent. Background technique [0002] In recent years, due to the rapid development of cities and industries, as well as the extensive use of chemical fertilizers in agriculture, a large amount of sewage has been discharged into water bodies, making many water bodies eutrophic. At present, measures to control water eutrophication mainly include: traditional nutrient salt control measures; external pollution interception, sewage diversion and sewage phosphorus removal; dredging and nutrient passivation for endogenous pollution A series of measures such as aeration, bottom aeration and dilution and scouring; direct algae removal measures such as chemical algae killing and mechanical algae fishing; biological control measures to control water body nutrients and phytoplankton with zooplankton, f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/125
Inventor 邱晓力叶俊英陈欢郭红樱
Owner 浙江天科高新技术发展有限公司
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